Effects of polymorphism in the 5′-flanking region of the IGF-I gene on milk-production traits in Chinese Holstein cattle

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Contents Vol 52(9)

doi:10.1071/AN12018

Food, Fibre and Pharmaceuticals from Animals

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Effects of polymorphism in the 5′-flanking region of the IGF-I gene on milk-production traits in Chinese Holstein cattle

M. A. Alim ^A ^C, Yan Xie ^A ^C, Yipeng Fan ^A, Xiaoping Wu ^A, Yi Zhang ^A, Dongxiao Sun ^A ^D, Shengli Zhang ^A ^D, Yuan Zhang ^A, Qin Zhang ^A and Lin Liu ^B

^A College of Animal Science and Technology, Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, National Engineering Laboratory for Animal Breeding, China Agricultural University, Beijing 100193, China.
^B Beijing Dairy Cattle Center, Beijing 100085, China.
^C These authors contributed equally to this work.
^D Corresponding author. Email: sundx@cau.edu.cn; zhangslcau@cau.edu.cn

Animal Production Science 52(9) 795-798 http://dx.doi.org/10.1071/AN12018
Submitted: 14 January 2012 Accepted: 9 February 2012 Published: 19 June 2012





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Abstract

Milk and milk products are major components of human dietary intake and have a potential influence on health. In our experiment, associations between insulin-like growth factor 1 (IGF-I) gene polymorphism and milk-production traits were analysed in Chinese Holstein cattle. A polymorphism, transition at position g.1407 T > C, was identified in the 5′-flanking region of the IGF-I gene by pooled DNA sequencing. The identified single-nucleotide polymorphism (SNP) was genotyped by matrix-assisted laser desorption–ionisation time-of-flight mass spectrometry (MALDI–TOF MS) methods from 752 individuals. Significant associations between IGF-I genotypes and 305-day milk yield, fat yield and protein yield were found. Homozygous cows with TT genotype showed the highest milk, fat and protein yields, with increases of 532.75 kg, 23.57 kg and 14.69 kg, respectively, as compared with homozygous CC cows. Heterozygous CT cows had intermediate yields. Allele substitution showed that the C allele decreased milk yield (255.23 kg), fat yield (11.37 kg) and protein yield (7.05 kg), whereas it increased protein percentage (0.01%). The results suggest that IGF-I is a candidate gene that influences milk-production traits, and provides useful information to be implemented in breeding programs to improve the performance of the Chinese Holstein.

Additional keywords: single-nucleotide polymorphisms.

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