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RESEARCH ARTICLE

Furanocoumarins in tedera do not affect ruminal fermentation in continuous culture

M. H. Ghaffari A , Z. Durmic A C F , D. Real B C D E , P. Vercoe A C , G. Smith B and C. Oldham B
+ Author Affiliations
- Author Affiliations

A School of Animal Biology, The University of Western Australia M085, 35 Stirling Highway, Crawley, WA 6009, Australia.

B Department of Agriculture and Food, Western Australia (DAFWA), 3 Baron-Hay Court, South Perth, WA 6151, Australia.

C Future Farm Industries Cooperative Research Centre, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

D School of Plant Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

E Centre for Legumes in Mediterranean Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

F Corresponding author. Email: zoey.durmic@uwa.edu.au

Animal Production Science 55(4) 544-550 https://doi.org/10.1071/AN13335
Submitted: 8 August 2013  Accepted: 20 January 2014   Published: 25 March 2014

Abstract

The objective of this study was to investigate the effects of the forage shrub tedera (Bituminaria bituminosa) on nutrient digestibility, rumen microbial fermentation and furanocoumarins degradation in the rusitec. The variables were measured in fermentation liquid on Days 13 and 17 and were compared with a control (lucerne hay). Overall, tedera had greater (P < 0.05) neutral detergent fibre and acid detergent fibre digestibility than lucerne hay on both days of the experiment, but on Day 17 it had lower (P < 0.01) dry matter and crude protein digestibility than lucerne hay. There were no significant differences in concentration of NH3-N and pH between treatments, but NH3-N concentrations in both treatments were lower (P < 0.05) on Day 17 than on Day 13. The concentration of total volatile fatty acids in vessels were not affected by treatments, but the concentration of acetate was lower and acetate-to-propionate ratio higher (P < 0.05) in tedera than lucerne hay on Day 13 of the experiment. Furanocoumarins were detected in the tedera treatment only. Their concentration in the fermentation liquid increased immediately after the addition of the plant material to the fermenter, reaching highest concentrations after 2 h. These concentrations gradually declined over the next two sampling times, but 6 h after the ‘feeding’, they were still detectable in the fermentation liquid. It was concluded that (i) tedera had in vitro digestibility and fermentability variables comparable to lucerne; (ii) furanocoumarins were degraded in the fermentation fluid, and (iii) furanocoumarins from tedera did not seem to impede microbial fermentation. Tedera may provide an alternative feed source to hay and grain for filling the summer–autumn feed gap without negatively affecting nutrient digestibility and rumen microbial fermentation.


References

Alvarez S, Mendez P, Diaz C, Fresno M (2004) Ingestión, composición química y digestibilidad in vivo de tedera (Bituminaria bituminosa (L.) Stirton). In ‘Pastos y Ganadería Extensiva. Actas. XLIV Reunión Científica de la Reunión Científica de la Sociedad Española para el Estudio de los Pastos (S.E.E.P.), Salamanca, Spain’. pp. 337–340.

AOAC (1990) ‘Official methods of analysis.’ 15th edn. (Association of Official Analytical Chemists: Arlington, VA)

Bhatta R, Tajima K, Takusari N, Higuchi K, Enishi O, Kurihara M (2006) Comparison of sulfur hexafluoride tracer technique, rumen simulation technique and in vitro gas production techniques for methane production from ruminant feeds. International Congress Series 1293, 58–61.
Comparison of sulfur hexafluoride tracer technique, rumen simulation technique and in vitro gas production techniques for methane production from ruminant feeds.Crossref | GoogleScholarGoogle Scholar |

Busquet M, Calsamiglia S, Ferret A, Cardozo PW, Kamel C (2005) Effects of cinnamaldehyde and garlic oil on rumen microbial fermentation in a dual flow continuous culture. Journal of Dairy Science 88, 2508–2516.
Effects of cinnamaldehyde and garlic oil on rumen microbial fermentation in a dual flow continuous culture.Crossref | GoogleScholarGoogle Scholar | 15956313PubMed |

Cardozo PW, Calsamiglia S, Ferret A, Kamel C (2004) Effects of natural plant extracts on ruminal protein degradation and fermentation profiles in continuous culture. Journal of Animal Science 82, 3230–3236.
Effects of natural plant extracts on ruminal protein degradation and fermentation profiles in continuous culture.Crossref | GoogleScholarGoogle Scholar | 15542469PubMed |

Cherdthong A, Wanapat M (2010) Development of urea products as rumen slow-release feed for ruminant production: a review. Australian Journal of Basic and Applied Sciences 4, 2232–2241.

Czerkawski JW, Breckenridge G (1977) Design and development of a long-term rumen simulation technique (Rusitec). The British Journal of Nutrition 38, 371–384.
Design and development of a long-term rumen simulation technique (Rusitec).Crossref | GoogleScholarGoogle Scholar | 588537PubMed |

Diawara PMM, Kulkosky PPJ (2003) Reproductive toxicity of the psoralens. Pediatric Pathology Molecular Medicine 22, 247–258.

Durmic Z, Hutton P, Revell DK, Emms J, Hughes S, Vercoe PE (2010) In vitro fermentative traits of Australian woody perennial plant that may be considered as potential sources of feed for grazing ruminants. Animal Feed Science and Technology 160, 98–109.
In vitro fermentative traits of Australian woody perennial plant that may be considered as potential sources of feed for grazing ruminants.Crossref | GoogleScholarGoogle Scholar |

Elizalde JC, Merchen NR, Faulkner DB (1999) Supplemental cracked corn for steers fed fresh alfalfa. I. Effects on digestion of organic matter, fiber, and starch. Journal of Animal Science 77, 457–466.

Finlayson J, Real D, Nordblom T, Revell C, Ewing M, Kingwell R (2012) Farm level assessments of a novel drought tolerant forage: Tedera (Bituminaria bituminosa C.H. Stirt var. albomarginata). Agricultural Systems 112, 38–47.
Farm level assessments of a novel drought tolerant forage: Tedera (Bituminaria bituminosa C.H. Stirt var. albomarginata).Crossref | GoogleScholarGoogle Scholar | http://www.sciencedirect.com/science/article/pii/S0308521X12000868.

Gulumser E, Acar Z (2012) Morphological and chemical characters of Bituminaria bituminosa (L.) C.H. (Stirton) grown naturally in Middle Black Sea region. Turkish Journal of Field Crops 17, 101–104.

Gutman M, Perevolotsky A, Sternberg M (2000) Grazing effects on a perennial legume, Bituminaria bituminosa (L.) Stirton, in a Mediterranean rangeland. Cahiers Options Mediter 45, 299–303.
Grazing effects on a perennial legume, Bituminaria bituminosa (L.) Stirton, in a Mediterranean rangeland.Crossref | GoogleScholarGoogle Scholar |

Littell RC, Henry PR, Ammerman CB (1998) Statistical analysis of repeated measures data using SAS procedures. Journal of Animal Science 76, 1216–1231.

Makkar HPS, Blümmel M, Becker K (1995) In vitro effects and interactions of tannins and saponins and fate of tannins in rumen. Journal of the Science of Food and Agriculture 69, 481–493.
In vitro effects and interactions of tannins and saponins and fate of tannins in rumen.Crossref | GoogleScholarGoogle Scholar |

Martínez-Fernández D, Walker DJ, Romero-Espinar P, Flores P, del Río JA (2011) Physiological responses of Bituminaria bituminosa to heavy metals. Journal of Plant Physiology 168, 2206–2211.
Physiological responses of Bituminaria bituminosa to heavy metals.Crossref | GoogleScholarGoogle Scholar | 21937143PubMed |

Mathison GW, Soofi-Siawash R, Klita PT, Okine EK, Sedgwick G (1999) Degradability of alfalfa saponins in the digestive tract of sheep and their rate of accumulation in rumen fluid. Canadian Journal of Animal Science 79, 315–319.
Degradability of alfalfa saponins in the digestive tract of sheep and their rate of accumulation in rumen fluid.Crossref | GoogleScholarGoogle Scholar |

McDougall EI (1948) Studies on ruminant saliva. The composition and output of sheep’s saliva. Biochemical Journal 43, 99–109.

McSweeney CS, Palmer B, McNeil DM, Krause DO (2001) Microbial interactions with tannins: nutritional consequences for ruminants. Animal Feed Science and Technology 91, 83–93.
Microbial interactions with tannins: nutritional consequences for ruminants.Crossref | GoogleScholarGoogle Scholar |

Méndez P, Santos A, Correal E, Ríos S (2006) Agronomic traits as forage crops of nineteen populations of Bituminaria bituminosa. Grassland Science in Europe 11, 300–302.

Mertens DR, Allen M, Carmany J, Clegg J, Davidowicz A, Drouches M, Frank K, Gambin D, Garkie M, Gildemeister B, Jeffress D, Jeon CS, Jones D, Kaplan D, Kim GN, Kobata S, Main D, Moua X, Paul B, Robertson J, Taysom D, Thiex N, Williams J, Wolf M (2002) Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study. The AOAC Official Methods of Analysis 85, 1217–1240.

Newbold CJ, El Hassan SM, Wang J, Ortega M, Wallace RJ (1997) Influence of foliage from African multipurpose trees on activity of rumen protozoa and bacteria. The British Journal of Nutrition 78, 237–249.
Influence of foliage from African multipurpose trees on activity of rumen protozoa and bacteria.Crossref | GoogleScholarGoogle Scholar | 9301414PubMed |

Oertli EH, Green GS, Griffith DG, Fowlks WL (1984) Linear furocoumarins and other constituents from Thamnosma texana. Phytochemistry 23, 439–441.
Linear furocoumarins and other constituents from Thamnosma texana.Crossref | GoogleScholarGoogle Scholar |

Oldham CM, Real D, Bailey HJ, Thomas D, Van Burgel AJ, Vercoe P, Correal E, Rios S (2013) Australian and Spanish scientists are collaborating in the domestication of tedera: young merino sheep grazing a monoculture of tedera in autumn showed preference for certain accessions but no signs of ill health. Crop and Pasture Science 64, 399–408.
Australian and Spanish scientists are collaborating in the domestication of tedera: young merino sheep grazing a monoculture of tedera in autumn showed preference for certain accessions but no signs of ill health.Crossref | GoogleScholarGoogle Scholar |

Pecetti L, Tava A, Pagnotta MA, Russi L (2007) Variation in forage quality and chemical composition among Italian accessions of Bituminaria bituminosa (L.) Stirt. Journal of the Science of Food and Agriculture 87, 985–991.
Variation in forage quality and chemical composition among Italian accessions of Bituminaria bituminosa (L.) Stirt.Crossref | GoogleScholarGoogle Scholar |

Pilajun P, Wanapat M (2011) Effect of coconut oil and mangosteen peel supplementation on ruminal fermentation, microbial population, and microbial protein synthesis in swamp buffaloes. Livestock Science 141, 148–154.
Effect of coconut oil and mangosteen peel supplementation on ruminal fermentation, microbial population, and microbial protein synthesis in swamp buffaloes.Crossref | GoogleScholarGoogle Scholar |

Real D, Correal E, Méndez P, Santos A, Ríos S, Sternberg M, Dini-Papanastasi O, Pecetti L, Tava A (2009) Bituminaria bituminosa C.H. Stirton. Available at http://www.fao.org/ag/agp/AGPC/doc/Gbase/new_species/tedera/bitbit.htm [Verified 28 February 2014]

Russell JB, Muck RE, Weimer PJ (2009) Quantitative analysis of cellulose degradation and growth of cellulolytic bacteria in the rumen. FEMS Microbial Ecology 67, 183–197.
Quantitative analysis of cellulose degradation and growth of cellulolytic bacteria in the rumen.Crossref | GoogleScholarGoogle Scholar |

SAS (2004) ‘Statistical analysis systems. Version 9.2.’ (SAS Institute: Cary, NC)

Sternberg M, Gishri N, Mabjeesh SJ (2006) Effects of grazing on Bituminaria bituminosa (L) Stirton: a potential forage crop in Mediterranean grasslands. Journal Agronomy Crop Science 192, 399–407.
Effects of grazing on Bituminaria bituminosa (L) Stirton: a potential forage crop in Mediterranean grasslands.Crossref | GoogleScholarGoogle Scholar |

Suriyagoda LDB, Real D, Renton M, Lambers H, Ryan MH (2013) Establishment, survival, and herbage production of novel, summer-active perennial pasture legumes in the low-rainfall cropping zone of Western Australia as affected by plant density and cutting frequency. Crop and Pasture Science 64, 71–85.
Establishment, survival, and herbage production of novel, summer-active perennial pasture legumes in the low-rainfall cropping zone of Western Australia as affected by plant density and cutting frequency.Crossref | GoogleScholarGoogle Scholar |

Susmel P, Stefanon B, Mills CR, Spenghero M (1990) Rumen degradability of organic matter, nitrogen and fibre fractions forages. Animal Production 51, 515–526.
Rumen degradability of organic matter, nitrogen and fibre fractions forages.Crossref | GoogleScholarGoogle Scholar |

Tava A, Pecetti L, Ricci M, Pagnotta MA, Russi L (2007) Volatile compounds from leaves and flowers of Bituminaria bituminosa (L.) Stirt. (Fabaceae) from Italy. Flavour and Fragrance Journal 22, 363–370.
Volatile compounds from leaves and flowers of Bituminaria bituminosa (L.) Stirt. (Fabaceae) from Italy.Crossref | GoogleScholarGoogle Scholar |

Ventura MR, Castanon JIR, Pieltain MC, Flores MP (2004) Nutritive value of forage shrubs Bituminaria bituminosa, Rumex lunarian, Acacia salicina, Cassia sturtii and Adenocorpus foliosus. Small Ruminant Research 52, 13–18.
Nutritive value of forage shrubs Bituminaria bituminosa, Rumex lunarian, Acacia salicina, Cassia sturtii and Adenocorpus foliosus.Crossref | GoogleScholarGoogle Scholar |

Ventura MR, Castanon JIR, Mendez P (2009) Effect of season on tedera (Bituminaria bituminosa) intake by goats. Animal Feed Science and Technology 153, 314–319.
Effect of season on tedera (Bituminaria bituminosa) intake by goats.Crossref | GoogleScholarGoogle Scholar |

Walker DJ, Martínez-Fernández D, Correal E, Romero-Espinar P, Antonio del Río J (2012) Accumulation of furanocoumarins by Bituminaria bituminosa in relation to plant development and environmental stress. Plant Physiology and Biochemistry 54, 133–139.
Accumulation of furanocoumarins by Bituminaria bituminosa in relation to plant development and environmental stress.Crossref | GoogleScholarGoogle Scholar | 22459509PubMed |

Wanapat M, Kongmun P, Poungchompu O, Cherdthong A, Khejornsart P, Pilajun R, Kaenpakdee S (2012) Effects of plants containing secondary compounds and plant oils on rumen fermentation and ecology. Tropical Animal Health and Production 44, 399–405.
Effects of plants containing secondary compounds and plant oils on rumen fermentation and ecology.Crossref | GoogleScholarGoogle Scholar | 21842136PubMed |