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RESEARCH ARTICLE

Fatty acids composition, cholesterol and vitamin E contents of Longissimus dorsi and Semitendinosus muscles of Suino Nero Lucano pigs slaughtered at two different weights

Annamaria Perna A B , Amalia Simonetti A , Immacolata Intaglietta A and Emilio Gambacorta A
+ Author Affiliations
- Author Affiliations

A School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Viale dell’Ateneo Lucano, 10 – 85100 Potenza, Italy.

B Corresponding author. Email: anna.perna@unibas.it

Animal Production Science 55(8) 1037-1043 https://doi.org/10.1071/AN14036
Submitted: 24 January 2014  Accepted: 28 May 2014   Published: 1 October 2014

Abstract

The nutritional quality of the lipid fraction of two muscles (Longissimus dorsi and Semitendinous) from Italian autochthonous genotype Suino Nero Lucano pigs slaughtered at two different weights was evaluated. Meat of Suino Nero Lucano pig showed a relatively low content of cholesterol and a higher proportion of unsaturated (UFA) than saturated fatty acids (SFA). Total cholesterol content was influenced by muscle, being higher in Longissimus dorsi (LD) than in Semitendinous (ST) muscle. No significant effects related to slaughter weight or muscle were found regarding vitamin E content. Slaughter weight strongly influenced n-3 and n-6 polyunsaturated fatty acids (PUFA) contents that decreased with increasing weight, and consequently, PUFA/SFA ratio. Muscle markedly influenced the contents of SFA, monounsaturated fatty acids (MUFA), and PUFA, and the dietetic properties of the meat. ST muscle, compared with the LD muscle, showed higher PUFA/SFA and PUFA n-6/PUFA n-3 ratios, and lower atherogenic and thrombogenic indices.


References

Alfonso L, Mourot J, Insausti K, Mendizabal JA, Arana A (2005) Comparative description of growth, fat deposition, carcass and meat quality characteristics of Basque and Large White pigs. Animal Research 54, 33–42.
Comparative description of growth, fat deposition, carcass and meat quality characteristics of Basque and Large White pigs.Crossref | GoogleScholarGoogle Scholar |

Andrés AI, Cava R, Mayoral AI, Tejeda JF, Morcuende D, Ruiz J (2001) Oxidative stability and fatty acid composition of pig muscles as affected by rearing system, crossbreeding and metabolic type of muscle fibre. Meat Science 59, 39–47.
Oxidative stability and fatty acid composition of pig muscles as affected by rearing system, crossbreeding and metabolic type of muscle fibre.Crossref | GoogleScholarGoogle Scholar | 22062504PubMed |

AOAC (1995) ‘Official methods of analysis.’ 16th edn (Association of Official Analytical Chemists: Arlington, VA)

Barja G, Cadenas S, Rojas C, Perez-Campo R, Lopez-Torres M, Prot J, Pamplona R (1996) Effect of dietary vitamin E levels on fatty acid profiles and nonenzymatic lipid peroxidation in the Guinea pig liver. Lipids 31, 963–970.
Effect of dietary vitamin E levels on fatty acid profiles and nonenzymatic lipid peroxidation in the Guinea pig liver.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28Xls1yltrk%3D&md5=48c12327981a5ac4dc950513873a22e8CAS | 8882976PubMed |

Bragagnolo N (2009) Cholesterol and cholesterol oxides in meat and meat products. In ‘Handbook of muscle foods analysis’. (Eds LML Nollet, F Toldra) pp 187–219 (CRC Press: Florida)

Candek-Potokar M, Monin G, Zlender B (2002) Pork quality, processing, and sensory characteristics of dry-cured hams as influenced by Duroc crossing and sex. Journal of Animal Science 80, 988–996.

Cava R, Estévez M, Ruiz J, Morcuende D (2003) Physicochemical characteristics of three muscles from free-range reared Iberian pigs slaughtered at 90 kg live weight. Meat Science 63, 533–541.
Physicochemical characteristics of three muscles from free-range reared Iberian pigs slaughtered at 90 kg live weight.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XptlWjt70%3D&md5=b0058102174948d191abdeea2aaf7811CAS | 22062524PubMed |

Chan KM, Decker EA (1994) Endogenous skeletal muscle antioxidant. Critical Reviews in Food Science and Nutrition 34, 403–426.
Endogenous skeletal muscle antioxidant.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXovFyjtA%3D%3D&md5=633da6a3dbeb20d074b6b9f6017936a0CAS | 7945896PubMed |

Chizzolini R, Zanardi E, Dorigoni V, Ghidini S (1999) Calorific value and cholesterol content of normal and low-fat meat and meat products. Trends in Food Science & Technology 10, 119–128.
Calorific value and cholesterol content of normal and low-fat meat and meat products.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXmvVKhs78%3D&md5=ac008aa88af6a150794caf9a26ec5614CAS |

De Smet S, Raes K, Demeyer D (2004) Meat fatty acid composition as affected by fatness and genetic factors: a review. Animal Research 53, 81–98.
Meat fatty acid composition as affected by fatness and genetic factors: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXltFymtrs%3D&md5=a69d3edac70d09f8b9f9b55db599118cCAS |

Department of Health (1994) Nutritional aspects of cardiovascular disease. Report of the cardiovascular review groupncommitee on medical aspects of foodpolicy. Report on health and social subjects, N° 46 (Department of Health: London)

Enser M (1991) Meat lipids. In ‘Developments in oils and fats’ (Ed. HRJ Blackie) pp 1–31 (Academic and Professional: Glasgow)

Fernández M, Ordóñez JA, Cambero I, Santos C, Pin C, de la Hoz L (2007) Fatty acid compositions of selected varieties of Spanish ham related to their nutritional implications. Food Chemistry 101, 107–112.
Fatty acid compositions of selected varieties of Spanish ham related to their nutritional implications.Crossref | GoogleScholarGoogle Scholar |

Food and Nutrition Board (2000) ‘Dietary reference intakes for vitamin C, vitamin E, selenium and carotenoids’. (The National Academy Press: Washington, DC)

Fortina R, Barbera S, Lussiana C, Mimosi A, Tassone S, Rossi A, Zanardi E (2005) Performances and meat quality of two Italian pig breeds fed diets for commercial hybrids. Meat Science 71, 713–718.
Performances and meat quality of two Italian pig breeds fed diets for commercial hybrids.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3Mbntl2gsw%3D%3D&md5=b56381e5299d366b662896bcd7d019b4CAS | 22061217PubMed |

Franci O, Pugliese C (2007) Italian autochthonous pigs: progress report and research perspectives. Italian Journal of Animal Science 6, 663–671.
Italian autochthonous pigs: progress report and research perspectives.Crossref | GoogleScholarGoogle Scholar |

Franci O, Bozzi R, Pugliese C, Acciaioli A, Campodoni G, Gandini G (2005) Performance of Cinta Senese pigs and their crosses with Large White. 1. Muscle and subcutaneous fat characteristics. Meat Science 69, 545–550.
Performance of Cinta Senese pigs and their crosses with Large White. 1. Muscle and subcutaneous fat characteristics.Crossref | GoogleScholarGoogle Scholar | 22062994PubMed |

Friesen KG, Nelssen JL, Goodband RD, Tokach MD, Unruh JA, Kropf DH, Kerr BJ (1995) The effect of dietary lysine on growth, carcass composition and lipid metabolism in high-lean growth gilts fed from 72 to 136 kg. Journal of Animal Science 73, 3392–3401.

González E, Tejeda JF (2007) Effects of dietary incorporation of different antioxidant extracts and free-range rearing on fatty acid composition and lipid oxidation of Iberian pig meat. Animal 1, 1060–1067.
Effects of dietary incorporation of different antioxidant extracts and free-range rearing on fatty acid composition and lipid oxidation of Iberian pig meat.Crossref | GoogleScholarGoogle Scholar | 22444809PubMed |

ISO (1978) Preparation of methyl esters of fatty acids. ISO 5509. In ‘Animal and vegetable fats and oils’. (International Organization for Standardization: Geneva) Available at http://www.iso.org/iso/iso_catalogue/catalogue_ics/catalogue_detail_ics.htm?csnumber=1150 [Verified 12 December 2013]

Jankowska B, Zakęś Z, Źmijewski T, Szczepkowski M (2010) Fatty acid profile of muscles, liver and mesenteric fat in wild and reared perch (Perca fluviatilis L.). Food Chemistry 118, 764–768.
Fatty acid profile of muscles, liver and mesenteric fat in wild and reared perch (Perca fluviatilis L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtV2jt7%2FL&md5=2702a951f7d6bcd954e49cb9192a5c9fCAS |

Jiang J, Bjoerck L, Fonden R, Emanuelson M (1996) Occurrence of conjugated cis-9, trans-11-octadecadienoic acid in bovine milk: effects of feed and dietary regimen. Journal of Dairy Science 79, 438–445.
Occurrence of conjugated cis-9, trans-11-octadecadienoic acid in bovine milk: effects of feed and dietary regimen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XisFGms7w%3D&md5=c0b518057b8a92b85fad37d10a964346CAS | 8708105PubMed |

Jiménez-Colmenero F, Carballo J, Cofrades S (2001) Healthier meat and meat products: their role as functional foods. Meat Science 59, 5–13.
Healthier meat and meat products: their role as functional foods.Crossref | GoogleScholarGoogle Scholar | 22062500PubMed |

Kang YK, Choi YM, Lee SH, Choe JH, Hong KC, Kim BC (2011) Effects of myosin heavy chain isoforms on meat quality, fatty acid composition, and sensory evaluation in Berkshire pigs. Meat Science 89, 384–389.
Effects of myosin heavy chain isoforms on meat quality, fatty acid composition, and sensory evaluation in Berkshire pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtFSqsrvK&md5=78feb5fbc0d9ee3625cdfa2508493b91CAS | 21636221PubMed |

Leseigneur-Meynier A, Gandemer G (1991) Lipid composition of pork muscle in relation to the metabolic type of the fibres. Meat Science 29, 229–241.
Lipid composition of pork muscle in relation to the metabolic type of the fibres.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXmsFCmurg%3D&md5=ab22f978f25786c89199876031ca8773CAS | 22061275PubMed |

Lo Fiego DP (1996) Carcass fatness and lipid quality in the heavy pig. Meat Focus International 8, 261–263.

Madonia G, Diaferia C, Moretti VM, Margiotta S, Manganelli E, Pruiti V, Caprino F, D’Amico A (2007) Nero Siciliano pigs proposed as a traditional quality product: comparison between salami made from black pig’s meat and white pig’s meat. Options Méditerranéennes 76, 251–257.

Morcuende D, Estévez M, Ruiz J, Cava R (2003) Oxidative and lipolytic deterioration of different muscles from free-range reared Iberian pigs under refrigerated storage. Meat Science 65, 1157–1164.
Oxidative and lipolytic deterioration of different muscles from free-range reared Iberian pigs under refrigerated storage.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXkvFGlur8%3D&md5=c9e1b434db4d261cfa2b839a4f4aed80CAS | 22063698PubMed |

Morrissey PA, Buckley DL, Sheehy PJA, Monahan FJ (1994) Vitamin E and meat quality. The Proceedings of the Nutrition Society 53, 289–295.
Vitamin E and meat quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXptFCitQ%3D%3D&md5=beaa2bebb2aaa19e932d27d84479bd2dCAS | 7972143PubMed |

Mourot J, Hermier D (2001) Lipids in monogastric animal meat. Reproduction, Nutrition, Development 41, 109–118.
Lipids in monogastric animal meat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXltFWhs7s%3D&md5=c73a024626042b149ce6ae4dc84fe403CAS | 11434515PubMed |

Muriel E, Antequera T, Ruíz J (2002) Efecto del tipo de músculo sobre parámetros de calidad en carne fresca de cerdo Ibérico. Ciencia y Tecnología Alimentaria 3, 241–247.
Efecto del tipo de músculo sobre parámetros de calidad en carne fresca de cerdo Ibérico.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XmtFeisb4%3D&md5=64ec01fe473a801480ad657de56e5e8aCAS |

Nürnberg K, Wegner J, Ender K (1998) Factors influencing fat composition in muscle and adipose tissue of farm animals. Livestock Production Science 56, 145–156.
Factors influencing fat composition in muscle and adipose tissue of farm animals.Crossref | GoogleScholarGoogle Scholar |

Parra D, Bandarra N, Kiely M, Thorsdottir I, Martinez J (2007) Impact of fish intake on oxidative stress when included into a moderate energy-restricted program to treat obesity. European Journal of Nutrition 46, 460–467.
Impact of fish intake on oxidative stress when included into a moderate energy-restricted program to treat obesity.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXht12nu70%3D&md5=6c770208a375fae13e486a68cfedd069CAS | 18026868PubMed |

Parthasarathy S, Barnett J, Fong LG (1990) High-density lipoprotein inhibits the oxidative modification of low-density lipoprotein. Biochimica et Biophysica Acta 1044, 275–283.
High-density lipoprotein inhibits the oxidative modification of low-density lipoprotein.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXkt1Wlsb4%3D&md5=6c22dc163dd5781fd4ae6e3eb738f15fCAS | 2344447PubMed |

Perna A, Freschi P, Gambacorta E, Cosentino E (1997) Confronto tra due metodi di stima della fitomassa pabulare in un pascolo dell’appennino meridionale. In ‘Parliamo di alimentazione animale e ambiente.’ pp. 205–226 (Atti del Convegno Nazionale: Italy)

Piironen V, Toivo J, Lampi A-M (2002) New data for cholesterol contents in meat, fish, milk, eggs and their products consumed in Finland. Journal of Food Composition and Analysis 15, 705–713.
New data for cholesterol contents in meat, fish, milk, eggs and their products consumed in Finland.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XpsF2qur0%3D&md5=a89087b097ceb1925ae69794d45d2aa0CAS |

Prates JAM, Quaresma MAG, Bessa RJB, Fontes CMGA, Alfaia CMPM (2006) Simultaneous HPLC quantification of total cholesterol, tocopherols and [beta]-carotene in Barrosã-PDO veal. Food Chemistry 94, 469–477.
Simultaneous HPLC quantification of total cholesterol, tocopherols and [beta]-carotene in Barrosã-PDO veal.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXms1yjs74%3D&md5=4f5fb6fe48d28a8dffa28ea3e7babc9eCAS |

Pugliese C, Sirtori F (2012) Quality of meat and meat products produced from southern European pig breeds. Meat Science 90, 511–518.
Quality of meat and meat products produced from southern European pig breeds.Crossref | GoogleScholarGoogle Scholar | 22030111PubMed |

Raj St, Skiba G, Weremko D, Fandrejewski H, Migdał W, Borowiec F, Poławska E (2010) The relationship between the chemical composition of the carcass and the fatty acid composition of intramuscular fat and backfat of several pig breeds slaughtered at different weights. Meat Science 86, 324–330.
The relationship between the chemical composition of the carcass and the fatty acid composition of intramuscular fat and backfat of several pig breeds slaughtered at different weights.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXptVejs7k%3D&md5=96708ec91e0fe406f6a8094d7ec8da57CAS | 20665991PubMed |

Realini CE, Pérez-Juan M, Gou P, Díaz I, Sárraga C, Gatellier P, García-Regueiro JA (2013) Characterization of Longissimus thoracis, Semitendinosus and Masseter muscles and relationships with technological quality in pigs. 2. Composition of muscles. Meat Science 94, 417–423.
Characterization of Longissimus thoracis, Semitendinosus and Masseter muscles and relationships with technological quality in pigs. 2. Composition of muscles.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXls1SgsLc%3D&md5=b5f2d07339669770b7a6001e1b46828bCAS | 23566688PubMed |

Rey AI, Daza A, López-Carrasco C, López-Bote CJ (2006) Feeding Iberian pigs with acorns and grass in either free-range or confinement affects the carcass characteristics and fatty acids and tocopherols accumulation in Longissimus dorsi miscle and backfat. Meat Science 73, 66–74.
Feeding Iberian pigs with acorns and grass in either free-range or confinement affects the carcass characteristics and fatty acids and tocopherols accumulation in Longissimus dorsi miscle and backfat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtFWgtrk%3D&md5=8d984fb700085d4f149f542df04f3515CAS | 22062055PubMed |

Roynette CE, Calder PC, Dupertuis YM, Pichard C (2004) n-3 Polyunsaturated fatty acids and colon cancer prevention. Clinical Nutrition (Edinburgh, Lothian) 23, 139–151.
n-3 Polyunsaturated fatty acids and colon cancer prevention.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXitFKgtLc%3D&md5=e63ef862bab7f31c338a87181eeff674CAS |

Salvatori G, Filetti F, Di Cesare C, Pilla F, Oriani G (2008) Lipid composition of meat and backfat from Casertana purebred and crossbred pigs reared outdoors. Meat Science 80, 623–631.
Lipid composition of meat and backfat from Casertana purebred and crossbred pigs reared outdoors.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtVKmtbvI&md5=8af841bbd42870d5efc218a2f70ce77eCAS | 22063574PubMed |

Santos-Silva J, Bessa RJB, Santos-Silva F (2002) Effect of genotype, system and slaughter weight on the quality of light lambs: II. Fatty acid composition of meat. Livestock Production Science 77, 187–194.
Effect of genotype, system and slaughter weight on the quality of light lambs: II. Fatty acid composition of meat.Crossref | GoogleScholarGoogle Scholar |

SAS Institute (1996) ‘SAS User’s Guide: Statistics. Version 7.’ (SAS Institute Inc.: Cary, NC)

Simopoulos AP (1999) Essential fatty acids in health and chronic disease. The American Journal of Clinical Nutrition 70, 560S–569S.

Stajić S, Živković D, Perunović M, Šobajić S, Vranic D (2011) Cholesterol content and atherogenicity of fermented sausages made of pork meat from various breeds. Procedia Food Science 1, 568–575.
Cholesterol content and atherogenicity of fermented sausages made of pork meat from various breeds.Crossref | GoogleScholarGoogle Scholar |

Taylor RG (2004) Muscle fiber types and meat quality. In ‘Encyclopedia of meat sciences’. (Eds WK Jensen, C Devine, M. Dikeman) pp 876–882 (Elsevier Academic Press: Oxford)

Tejerina D, García-Torres S, Cabeza de Vaca M, Vázquez FM, Cava R (2012a) Study of variability in antioxidant composition and fatty acids profile of Longissimus dorsi and Serratus ventralis muscles from Iberian pigs reared in two different Montanera seasons. Meat Science 90, 414–419.
Study of variability in antioxidant composition and fatty acids profile of Longissimus dorsi and Serratus ventralis muscles from Iberian pigs reared in two different Montanera seasons.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsVWkt7%2FM&md5=4c26e4555b3b395c24a908e8f1f241e2CAS | 21924560PubMed |

Tejerina D, García-Torres S, Cabeza de Vaca M, Vázquez FM, Cava R (2012b) Effect of production system on physical-chemical, antioxidant and fatty acids composition of Longissimus dorsi and Serratus ventralis muscles from Iberian pig. Food Chemistry 133, 293–299.
Effect of production system on physical-chemical, antioxidant and fatty acids composition of Longissimus dorsi and Serratus ventralis muscles from Iberian pig.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XitVWqt7s%3D&md5=d271fa961de1693e97c2253732c4129cCAS |

Ulbricht TLV, Southgate DAT (1991) Coronary heart disease: seven dietary factors. The Lancet 338, 985–992.
Coronary heart disease: seven dietary factors.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XhtVCrur0%3D&md5=6a460d25baf13c05a457f99e85420ee9CAS |

United States Department of Agriculture (2012) National nutrient database for standard reference. USDA. Available at http://fnic.nal.usda.gov/food-composition. [Verified 18 November 2013]

Vestergaard M, Oksbjerg N, Henckel P (2000) Influence of feeding intensity, grazing and finishing feeding on muscle fibre characteristics and meat colour of semitendinosus, longissimus dorsi and supraspinatus muscles of young bulls. Meat Science 54, 177–185.
Influence of feeding intensity, grazing and finishing feeding on muscle fibre characteristics and meat colour of semitendinosus, longissimus dorsi and supraspinatus muscles of young bulls.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MbntlCgtw%3D%3D&md5=d0d841f609750d8e3769308d7e87327eCAS | 22060614PubMed |

World Health Organization (WHO) (2003) Diet, nutrition and the prevention of chronic diseases. Report of a Joint WHO/FAO Expert Consultation. WHO Technical Report Series No. 916. (World Health Organization: Geneva, Switzerland)

Young IS, Woodside JV (2001) Antioxidants in health and disease. Journal of Clinical Pathology 54, 176–186.
Antioxidants in health and disease.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3M7osFakuw%3D%3D&md5=ac6de78ba916d0aae8be07aa1e4d1dc9CAS | 11253127PubMed |

Zullo A, Barone CMA, Colatruglio P, Girolami A, Matassino D (2003) Chemical composition of pig meat from the genetic type Casertana and its crossbreeds. Meat Science 63, 89–100.
Chemical composition of pig meat from the genetic type Casertana and its crossbreeds.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MbnsVGksw%3D%3D&md5=a24eddde3571aa154acd1592fed6b7f2CAS | 22061990PubMed |