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RESEARCH ARTICLE

Effect of natural mating or laparoscopic artificial insemination in superovulated Santa Inês ewes on superovulatory response, fertility and embryo viability

J. T. M. Lima A , J. F. Fonseca B , M. F. A. Balaro A , L. V. Esteves A , F. O. Ascoli A , C. R. Leite A , A. C. S. Ribeiro A , K. F. Delgado A , J. M. G. Souza-Fabjan A C , R. A. Torres Filho A and F. Z. Brandão A
+ Author Affiliations
- Author Affiliations

A Faculdade de Medicina Veterinária, Universidade Federal Fluminense, CEP 24230-340, Niterói, RJ, Brazil.

B Embrapa Goats and Sheep, Estrada Sobral/Groaíras, CEP 62010-970, Sobral, CE, Brazil.

C Corresponding author. Email: joannavet@gmail.com

Animal Production Science 56(9) 1463-1468 https://doi.org/10.1071/AN14827
Submitted: 19 September 2014  Accepted: 10 February 2015   Published: 24 April 2015

Abstract

This study evaluated the effect of two mating methods (GNM: natural mating or GAI: laparoscopic artificial insemination) on superovulatory response, fertility and embryo yield in superovulated ewes. Fifteen non-pregnant Santa Inês ewes were superovulated and either mated by GNM or GAI in a crossover design. Oestrus was synchronised using intravaginal progestagen sponges for 6 days and on Day 5, 300 IU eCG and 0.0375 mg d-cloprostenol were given. Twelve hours after sponge removal, 0.025 mg gonadotropin-releasing hormone was administered. Superovulation started 48 h after gonadotropin-releasing hormone treatment, using 5 IU/kg follicle-stimulating hormone (pFSH). At the first pFSH dose, new sponges were inserted. At the fifth dose, 0.0375 mg cloprostenol was administered and the sponges were removed. The GNM was mated with rams every 12 h, until the end of oestrus. The ewes of GAI were laparoscopic inseminated with frozen–thawed semen 36 and 48 h after sponge removal. Ultrasonography was performed every 24 h from the beginning of oestrus synchronisation treatment and every 12 h from the second sponge removal to 2 days after the last pFSH dose. Six to seven days after mating, the number of corpora lutea (CL) was evaluated by laparoscopy and the females with > 4 CL were subjected to embryo collection. The interval from sponge removal to ovulation was shorter (P < 0.05) in the GNM. The overall superovulatory response was 63.3% (19/30), with 60.0% and 66.7% in GNM and GAI, respectively (P > 0.05). The number of recovered structures (6.4 ± 2.4 vs 4.5 ± 3.0), recovery rate (74.0 ± 16.0 vs 52.3 ± 26.5%), number of transferable embryos (3.0 ± 2.9 vs 3.6 ± 2.0) and viability rate (47.2 ± 45.3 vs 77.4 ± 37.1%) did not differ between GAI and GNM (P > 0.05). However, the GAI group showed a higher (P < 0.05) number of unfertilised oocytes (3.1 ± 3.1) and a higher non-fertilisation rate (47.1 ± 45.3%) than the GNM (0.9 ± 2.1 and 11.5 ± 21.5%). The mating method did not affect the superovulatory response, and production of viable embryos although the non-fertilisation rate has been inferior for the AI group.

Additional keywords: artificial insemination, estrus synchronisation, sheep, superovulation.


References

Arashiro EKN, Fonseca JF, Henry M, Figueira LM, Magão JVP, Oliveira DR, Esteves LV, Brandão FZ (2009) Effect of first follicular wave synchronization protocol on superovulatory response in Santa Inês ewes. Animal Reproduction 18, 433

Armstrong DT, Evans G (1983) Embryo transfer in sheep and goats. Theriogenology 19, 31–42.
Embryo transfer in sheep and goats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3sXms1ajsg%3D%3D&md5=262f10f0d35a1bcd5536f8185e462672CAS |

Azawi OI, Al-Mola MKMA (2011) A study on the effect of GnRH administration on the ovarian response and laparoscopic intrauterine insemination of Awassi ewes treated with eCG to induce superovulation. Tropical Animal Health and Production 43, 1351–1355.
A study on the effect of GnRH administration on the ovarian response and laparoscopic intrauterine insemination of Awassi ewes treated with eCG to induce superovulation.Crossref | GoogleScholarGoogle Scholar | 21509456PubMed |

Baldassare H (2008) Coleta e transferência de embrião. In ‘Reprodução ovina e caprina’. (Ed. EG Aisen) pp. 143–152. (Med. Vet.: São Paulo, Brazil)

Bari F, Khalid M, Haresign W, Murray A, Merrel B (2000) Effect of mating system, flushing procedure, progesterone dose, and donor ewe age on the yield and quality of embryos within a MOET program in sheep. Theriogenology 53, 727–742.
Effect of mating system, flushing procedure, progesterone dose, and donor ewe age on the yield and quality of embryos within a MOET program in sheep.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3c7pvFaitg%3D%3D&md5=8403126b27bc14ada4566d6377677dc0CAS | 10735039PubMed |

Bartlewski PM, Baby TE, Giffin JL (2011) Reproductive cycles in sheep. Animal Reproduction Science 124, 259–268.
Reproductive cycles in sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXlvFWhurw%3D&md5=01afac9783d8aa9a631d9e31e6379bacCAS | 21411253PubMed |

Cavalcanti AS, Brandão FZ, Nogueira LAG, Fonseca JF (2012) Effects of GnRH administration on ovulation and fertility in ewes subjected to estrous synchronization. Revista Brasileira de Zootecnia 41, 1412–1418.
Effects of GnRH administration on ovulation and fertility in ewes subjected to estrous synchronization.Crossref | GoogleScholarGoogle Scholar |

Cognié Y (1999) State of the art in sheep-goat embryo transfer. Theriogenology 51, 105–116.
State of the art in sheep-goat embryo transfer.Crossref | GoogleScholarGoogle Scholar | 10729066PubMed |

Cognié Y, Baril G, Poulin N, Mermillod P (2003) Current status of embryo technologies in sheep and goat. Theriogenology 59, 171–188.
Current status of embryo technologies in sheep and goat.Crossref | GoogleScholarGoogle Scholar | 12499027PubMed |

Cordeiro MF, Lima-Verde JB, Lopes-Júnior ES, Teixeira DIA, Farias LN, Salles HO, Simplício AA, Rondina D, Freitas VJF (2003) Embryo recovery rate in Santa Inês ewes subjected to successive superovulatory treatments with pFSH. Small Ruminant Research 49, 19–23.
Embryo recovery rate in Santa Inês ewes subjected to successive superovulatory treatments with pFSH.Crossref | GoogleScholarGoogle Scholar |

Delgadillo JA, Gelez H, Ungerfeld R, Hawken PAR, Martin GB (2009) The ‘male effect’ in sheep and goats – revisiting the dogmas. Behavioural Brain Research 200, 304–314.
The ‘male effect’ in sheep and goats – revisiting the dogmas.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXktlyqt78%3D&md5=e4fcee4ee6450b085b464c1a8279677cCAS | 19374015PubMed |

Evans G, Armstrong DT (1984) Reduction of sperm transport in ewes by superovulation treatments. Journal of Reproduction and Fertility 70, 47–53.
Reduction of sperm transport in ewes by superovulation treatments.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL2c7hsVKluw%3D%3D&md5=f63c93c6c22c403c9243eedcdb51e384CAS | 6420557PubMed |

Evans ACO, Duffy P, Hynes N, Boland MP (2000) Waves of follicle development during the estrous cycle in sheep. Theriogenology 53, 699–715.
Waves of follicle development during the estrous cycle in sheep.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3c7pvFaisA%3D%3D&md5=0edcc8e74607297dbff8b78d9eb320baCAS |

Ginther OJ, Kot K (1994) Follicular dynamics during the ovulatory season in goats. Theriogenology 42, 987–1001.
Follicular dynamics during the ovulatory season in goats.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD28zgtVelsg%3D%3D&md5=4869f790191268a6d229b0298319c293CAS | 16727603PubMed |

González-Bulnes A, Garcia-Garcia RM, Santiago-Moreno J, Lopez-Sebastian A, Cocero MJ, Baird DT (2002a) Patterns of follicular growth in superovulated sheep and influence on endocrine and ovarian response. Reproduction in Domestic Animals 37, 357–361.
Patterns of follicular growth in superovulated sheep and influence on endocrine and ovarian response.Crossref | GoogleScholarGoogle Scholar | 12464075PubMed |

González-Bulnes A, Santiago-Moreno J, Cocero MJ, Souza CJH, Groome NP, Garcia-Garcia RM, Lopez-Sebastian A, Baird DT (2002b) Measurement of inhibin A and follicular status predict the response of ewes to superovulatory FSH treatments. Theriogenology 57, 1263–1272.
Measurement of inhibin A and follicular status predict the response of ewes to superovulatory FSH treatments.Crossref | GoogleScholarGoogle Scholar | 12013446PubMed |

Gordon I (1997) ‘Controlled reproduction in sheep and goats. Vol. 2.’ (CAB International: New York, NY)

Haresign W (1992) Manipulation of reproduction in sheep. Journal of Reproduction and Fertility. Supplement 45, 127–139.

Houdeau E, Raynal P, Marnet PG, Germain G, Mormède P, Rossano B, Monnerie R, Prud’Homme MJ (2002) Plasma levels of cortisol and oxytocin, and uterine activity after cervical artificial insemination in the ewe. Reproduction, Nutrition, Development 42, 381–392.
Plasma levels of cortisol and oxytocin, and uterine activity after cervical artificial insemination in the ewe.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXkslWqtg%3D%3D&md5=3b22909d897a19e371d17b07490bc467CAS | 12510878PubMed |

Lucidi P, Barboni B, Mattioli M (2001) Ram-induced ovulation to improve artificial insemination. Theriogenology 55, 1797–1805.
Ram-induced ovulation to improve artificial insemination.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXkvFarsb8%3D&md5=ba1e09b1d0b698f92e1d87f21a6d5e6eCAS | 11414485PubMed |

Martin GB, Oldham CM, Lindsay DR (1981) Effect of stress due to laparoscopy on plasma cortisol levels, the preovulatory surge of LH and ovulation in the ewe. Theriogenology 16, 39–44.
Effect of stress due to laparoscopy on plasma cortisol levels, the preovulatory surge of LH and ovulation in the ewe.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3MXltlSnsLs%3D&md5=f4df35a5ddc80d56aedda6b7725c8bf4CAS | 16725617PubMed |

McKelvey WAC, Robinson JJ, Aitken RP, Henderson G (1985) The evaluation of a laparoscopic insemination technique in ewes. Theriogenology 24, 519–535.
The evaluation of a laparoscopic insemination technique in ewes.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD283pvVyktg%3D%3D&md5=83d9330ac09619994b31aff3fd8e99f7CAS |

Menchaca A, Vilariño M, Crispo M, Pinczak A, Rubianes E (2007) Day 0 protocol: superstimulatory treatment initiated in the absence of a large follicle improves ovarian response and embryo yield in goats. Theriogenology 68, 1111–1117.
Day 0 protocol: superstimulatory treatment initiated in the absence of a large follicle improves ovarian response and embryo yield in goats.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2srpvVOktQ%3D%3D&md5=13a8419f10933ca823f8fdde1899ca4cCAS | 17889305PubMed |

Menchaca A, Vilariño M, Pinczak A, Kmaid S, Saldaña JM (2009) Progesterone treatment, FSH plus eCG, GnRH administration, and Day 0 Protocol for MOET programs in sheep. Theriogenology 72, 477–483.
Progesterone treatment, FSH plus eCG, GnRH administration, and Day 0 Protocol for MOET programs in sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXptl2nsrs%3D&md5=cf74a8cf132d451f9e16a7a2726e3e3dCAS | 19515409PubMed |

Menchaca A, Vilariño M, Crispo M, de Castro T, Rubianes E (2010) New approaches to superovulation and embryo transfer in small ruminants. Reproduction, Fertility and Development 22, 113–118.
New approaches to superovulation and embryo transfer in small ruminants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXitlagu7w%3D&md5=6eab47cd80fc88b30967d8d6cedb5bd9CAS |

Morello HH, Chemineau P (2008) Características anatômicas e funcionais do sistema reprodutor da fêmea. In ‘Reprodução ovina e caprina’. (Ed. EG Aisen) pp. 11–25. (Med. Vet.: São Paulo, Brazil)

Naqvi SMK, Josh A, Das GK, Mittal JP (2001) Development and application of ovine reproductive technologies: an Indian experience. Small Ruminant Research 39, 199–208.
Development and application of ovine reproductive technologies: an Indian experience.Crossref | GoogleScholarGoogle Scholar |

Oliveira MEF (2011) State-of-the-art in the superovulation of ewes. Acta Scientiae Veterinariae 39, 29–35.

Oliveira MEF, Cordeiro MF, Ferreira RM, Souza SF, Pieroni JSP, Rodrigues LFS, Fonseca JFF, Vicente WRR (2012) Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG? Ciência Rural 42, 1077–1082.
Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhsleqtrfL&md5=c6c17b4fc090af27bf4ee38505e491dcCAS |

Papargiris MM, Rivalland ETA, Hemsworth PH, Morrissey AD, Tilbrook AJ (2011) Acute and chronic stress-like levels of cortisol inhibit the oestradiol stimulus to induce sexual receptivity but have no effect on sexual attractivity or proceptivity in female sheep. Hormones and Behavior 60, 336–345.
Acute and chronic stress-like levels of cortisol inhibit the oestradiol stimulus to induce sexual receptivity but have no effect on sexual attractivity or proceptivity in female sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtFarurjL&md5=a0bebb8011515bfc10f9ea5f31131f58CAS | 21741382PubMed |

Radford HM (1966) Pharmacological blockade of ovulation in the ewe. The Journal of Endocrinology 34, 135–136.
Pharmacological blockade of ovulation in the ewe.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaF28%2FltFOrtg%3D%3D&md5=74e564c13f3a0caf7331590874606dafCAS | 5948021PubMed |

Romano JE, Abella FD (1997) Effect of service on duration of oestrus and ovulation in dairy goats. Animal Reproduction Science 47, 107–112.
Effect of service on duration of oestrus and ovulation in dairy goats.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK2sznsl2isg%3D%3D&md5=cc1dd3aaf5381fff3cf8d52d7a89bf4bCAS | 9233510PubMed |

Romano JE, Christians C, Crabo B (2000) Continuous presence of rams hastens the onset of estrus in ewes synchronized during the breeding season. Applied Animal Behaviour Science 66, 65–70.
Continuous presence of rams hastens the onset of estrus in ewes synchronized during the breeding season.Crossref | GoogleScholarGoogle Scholar |

Rubianes E, Menchaca A (2006) Follicular dynamics, oestrous synchronisation and superovulation in sheep. Acta Scientiae Veterinariae 34, 251–261.

Rubianes E, Ibarra D, Ungerfeld R, Carbajal B, De Castro T (1995) Superovulatory response in anestrous ewes affected by the presence of a large follicle. Theriogenology 43, 465–472.
Superovulatory response in anestrous ewes affected by the presence of a large follicle.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD28zgtVeqtw%3D%3D&md5=c524072619c901552fd5dc2aab1e2fb5CAS | 16727638PubMed |

Rubianes E, Ungerfeld R, Viñoles C, Rivero A, Adams GP (1997) Ovarian response to gonadotropin treatment initiated relative to wave emergence in ultrasonographically monitored ewes. Theriogenology 47, 1479–1488.
Ovarian response to gonadotropin treatment initiated relative to wave emergence in ultrasonographically monitored ewes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXjvVyjurg%3D&md5=547ddb09851a08f08301eafb7b1525deCAS | 16728092PubMed |

Simonetti L, Forcada F, Rivera OE, Carou N, Alberio RH, Abecia JA, Palacin I (2008) Simplified superovulatory treatments in Corriedale ewes. Animal Reproduction Science 104, 227–237.
Simplified superovulatory treatments in Corriedale ewes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsVOmsLk%3D&md5=39b9dca748379e57c01b5b1a3f207aa9CAS | 17331680PubMed |

Simplício AA, Freitas VJF, Fonseca JF (2007) Biotécnicas da reprodução como técnicas de manejo reprodutivo em ovinos. Revista Brasileira de Reprodução Animal 31, 234–246.

Stringfellow DA, Seidel SM (1998) ‘Manual of the International Embryo Transfer Society.’ 3rd edn. (Savoy: Champaign, IL, USA)

Varago FC, Moustacas VS, Cruz BC, De Carvalho BC, Mendonça LF, Lagares MA, Henry MRJM (2009) Biotécnicas da reprodução aplicada a pequenos ruminantes. Brazilian Animal Science 8, 1–17.

Veiga-Lopez A, Encinas T, McNeilly AS, Gonzales-Bulnes A (2008) Timing of preovulatory LH surge and ovulation in superovulated sheep are affected by follicular status at start of the FSH treatment. Reproduction in Domestic Animals 43, 92–98.

Wagenmaker ER, Breen KM, Oakley AE, Pierce BN, Tilbrook AJ, Turner AI, Karsch FJ (2009) Cortisol interferes with the estradiol-induced surge of luteinizing hormone in the ewe. Biology of Reproduction 80, 458–463.
Cortisol interferes with the estradiol-induced surge of luteinizing hormone in the ewe.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXis1amtL0%3D&md5=412ba21eef7b2c8e8f5135de007a39baCAS | 19056703PubMed |

Whittingham DG (1971) Culture of mouse ova. Journal of Reproduction and Fertility 48, 7–21.