Fatty acid profile, carcass traits and meat quality of Nellore steers following supplementation with various lipid sources
Isabela P. C. Carvalho A , Giovani Fiorentini A B , Josiane F. Lage A , Juliana D. Messana A , Roberta C. Canesin A , Luis G. Rossi A , Ricardo A. Reis A and Telma T. Berchielli A
+ Author Affiliations
- Author Affiliations
A UNESP – University Estadual Paulista, Department of Animal Science, Jaboticabal, São Paulo, Brazil.
B Corresponding author. Email: giovanizoot@yahoo.com.br
Animal Production Science 57(6) 1170-1178 https://doi.org/10.1071/AN15149
Submitted: 18 March 2015 Accepted: 22 March 2016 Published: 24 August 2016
Abstract
The aim of the present study was to evaluate the fatty acid composition of meat and subcutaneous fat, carcass traits and meat quality of Nellore steers fed diets supplemented with various lipid sources. Forty-five young bulls, with average bodyweight of 441 ± 30 kg, were allotted into 10 paddocks, with five treatments, each consisting of two paddocks. The bulls were randomly assigned into one of the following five treatments, which consisted of four lipid sources: palm oil (PO), linseed oil, rumen protected fat (soybean-based oil), whole soybean, and a control (without additional fat). Trial duration was 120 days, which included 30 days of adaptation. Supplements were offered daily at 10 g/kg bodyweight per day. Dietary supplements for providing additional fat were formulated to consist of 10% ether extract. Lipid sources did not significantly affect the average daily gain (P = 0.797) or dressing percentage (P = 0.663). Supplementation with PO increased the concentrations of lauric acid (P = 0.036) and myristic acid (P < 0.001) in the muscle and subcutaneous fat. Animals supplemented with linseed oil had significantly higher concentrations of conjugated linolenic acid in the meat (P = 0.036) and fat (P = 0.049) than did control animals. In the present study, the use of various lipid sources in dietary supplements of grazing cattle during finishing period did not affect carcass traits or physical attributes of beef. This absence of statistical significance may be related to the minimum number of repeat paddocks (2) per treatment. Thus, differences that can have a practical significance were not evidenced by statistical analysis. The inclusion of PO and protected fatty acids derived from soybean oil (rumen-protected fat) is not recommended as a method to improve the lipid profile of meat and subcutaneous fat of Nellore cattle.
Additional keywords: Brachiaria brizantha, conjugated linolenic acid, linseed oil, palm oil, rumen-protected fat.
References
Aferri G, Leme PR, Silva SL, Putrino SM, Pereira ASC (2005) Performance and carcass characteristics of steers fed different fat sources. Revista Brasileira de Zootecnia 34, 1651–1658.| Performance and carcass characteristics of steers fed different fat sources.Crossref | GoogleScholarGoogle Scholar |
Aferri G, Leme PR, Pereira ASC, Putrino SM, Freitas Júnior JED, Rennó FP (2012) Fatty acid composition of the longissimus dorsi muscle in crossbred fed different sources of fatty acids. Revista Brasileira de Zootecnia 41, 1706–1712.
| Fatty acid composition of the longissimus dorsi muscle in crossbred fed different sources of fatty acids.Crossref | GoogleScholarGoogle Scholar |
Allingham PG, Harper GS, Hunter RA (1998) Effect of growth path on the tenderness of the semitendinosus muscle in Brahman-cross steers. Meat Science 48, 65–73.
| Effect of growth path on the tenderness of the semitendinosus muscle in Brahman-cross steers.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MbnsFaquw%3D%3D&md5=7fdad72ba7d20efb30f752e0949af407CAS | 22062879PubMed |
American Meat Science Association (1995) ‘Research guidelines for cookery, sensory evaluation and tenderness measurements of fresh meat.’ (National Livestock and Meat Board: Chicago, IL)
Association of Official Analytical Chemists (AOAC) (1990) ‘Official methods of analysis of the Association of Analytical Chemists.’ 14th edn. (AOAC: Washington, DC)
Belew JB, Brooks JC, McKenna DR, Savell JW (2003) Warner–Bratzler shear evaluations of 40 bovine muscles. Meat Science 64, 507–512.
| Warner–Bratzler shear evaluations of 40 bovine muscles.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MbnsFCqtQ%3D%3D&md5=0173354edff2f92af9719dcae8ad7ce3CAS | 22063134PubMed |
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37, 911–917.
| A rapid method of total lipid extraction and purification.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaG1MXhtVSgt70%3D&md5=c1d746a6458016be924540a5fe831f9aCAS | 13671378PubMed |
Bouton PE, Fisher AL, Harris PV, Baxter RI (1973) A comparison of the effects of some post-slaughter treatments on the tenderness of beef. Journal of Food Technology 8, 39–49.
| A comparison of the effects of some post-slaughter treatments on the tenderness of beef.Crossref | GoogleScholarGoogle Scholar |
Bowling RA, Riggs JK, Smith GC, Carpenter ZL, Reddish RL, Butler OD (1978) Production, carcass and palatability characteristics of steers produced by different management systems. Journal of Animal Science 46, 333–341.
Busboom JR, Miller GJ, Field RA, Crouse JD, Riley ML, Nelms GE, Ferrell CL (1981) Characteristics of fat from heavy ram and wether lambs. Journal of Animal Science 52, 83–92.
Coppock CE, Wilks DL (1991) Supplemental fat in high energy rations for lactating cows: effects on intake, digestion, milk yield, and composition. Journal of Animal Science 69, 3826–3837.
Cross HR, West RL, Dutson TR (1981) Comparison of methods for sarcomere length in beef semitendinosus muscle. Meat Science 5, 261–266.
| Comparison of methods for sarcomere length in beef semitendinosus muscle.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MbmvFClsA%3D%3D&md5=ecf1910510e6f592c0d73922a25f832eCAS | 22056091PubMed |
Crouse JD, Cundiff LV, Koch RM, Koohmaraie M, Seideman SC (1989) Comparisons of Bos indicus and Bos taurus inheritance for carcass beef characteristics and meat palatability. Journal of Animal Science 67, 2661–2668.
Dannenberger D, Nuernberg K, Nuernberg G, Ender C (2006) Carcass and meat quality of pasture × concentrate fed German Simmental and German Holstein bulls. Archives Animal Breeding 49, 315–328.
Diniz LL, Valadares Filho SC, Campos JMS, Valadares RFD, Da Silva LD, Monnerat JPIS, Benedeti PB, Oliveira AS, Pina DS (2010) Effects of castor meal on the growth performance and carcass characteristics of beef cattle. Asian–Australasian Journal of Animal Sciences 23, 1308–1318.
| Effects of castor meal on the growth performance and carcass characteristics of beef cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsFemur7J&md5=48e3ee17d7c19a1a9eb81d713ffe44e5CAS |
Fiorentini G, Santana MCA, Sampaio AAM, Reis RA, Ribeiro AF, Berchielli TT (2012a) Intake and performance of confined crossbred heifers fed different lipid sources. Revista Brasileira de Zootecnia 41, 1490–1498.
| Intake and performance of confined crossbred heifers fed different lipid sources.Crossref | GoogleScholarGoogle Scholar |
Fiorentini G, Berchielli TT, Santana MCA, Dian PHM, Reis RA, Sampaio AAM, Biehl MV (2012b) Qualitative characteristics of meat from confined crossbred heifers fed with lipid sources. Scientia Agricola 69, 336–344.
| Qualitative characteristics of meat from confined crossbred heifers fed with lipid sources.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhvVKgt7nJ&md5=c3f1afabaf6ce61fbec073c6766a0824CAS |
Folch J, Lees M, Stanley GHS (1957) A simple method for the isolation and purification of total lipids from animal tissues. The Journal of Biological Chemistry 226, 497–509.
Granit R, Angel S, Akiri B, Holzer Z, Aharoni Y, Orlov A, Kanner J (2001) Effects of vitamin E supplementation on lipid peroxidation and color retention of salted calf muscle from a diet rich in polyunsaturated fatty acids. Journal of Agricultural and Food Chemistry 49, 5951–5956.
| Effects of vitamin E supplementation on lipid peroxidation and color retention of salted calf muscle from a diet rich in polyunsaturated fatty acids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXotlWht7o%3D&md5=f40e560b06aaaf4ece9ffd8812e7b399CAS | 11743791PubMed |
Greiner SP, Rouse GH, Wilson DE, Cundiff LV, Wheeler TL (2003) Prediction of retail product weight and percentage using ultrasound and carcass measurements in beef cattle. Journal of Animal Science 81, 1736–1742.
Hocquette JF, Botreau R, Picard B, Jacquet A, Pethick DW, Scollan ND (2012) Opportunities for predicting and manipulating beef quality. Meat Science 92, 197–209.
| Opportunities for predicting and manipulating beef quality.Crossref | GoogleScholarGoogle Scholar | 22554471PubMed |
Houben JH, Van Dijk A, Eikelenboom G, Hoving-Bolink AH (2000) Effect of dietary vitamin E supplementation, fat level and packaging on colour stability and lipid oxidation in minced beef. Meat Science 55, 331–336.
| Effect of dietary vitamin E supplementation, fat level and packaging on colour stability and lipid oxidation in minced beef.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXktVensbc%3D&md5=001a5541b9ca9fde4753b5aac1a56d8cCAS | 22061291PubMed |
ISO (1978) Animal and vegetable fats and oils. Preparation of methyl esters of fatty acids. In ‘Method ISO 5509’. pp. 1–6. (International Organization for Standardization: Geneva, Switzerland)
Koohmaraie M (1996) Biochemical factors regulating the toughening and tenderization process of meat. Meat Science 43, S193–S201.
| Biochemical factors regulating the toughening and tenderization process of meat.Crossref | GoogleScholarGoogle Scholar |
Kramer JKG, Fellner V, Dugan MER, Sauner FD, Mossoba MM (1997) Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis conjugated dieno and total trans fatty acids. Lipids 32, 1219–1228.
| Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis conjugated dieno and total trans fatty acids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXnsFaisbk%3D&md5=d147fa14069a47b037ab2e910442c147CAS |
Lage JF, Paulino PVR, Valadares Filho SC, Souza EJO, Duarte MS, Benedeti PDB, Souza NKP, Cox RB (2012) Influence of genetic type and level of concentrate in the finishing diet on carcass and meat quality traits in beef heifers. Meat Science 90, 770–774.
| Influence of genetic type and level of concentrate in the finishing diet on carcass and meat quality traits in beef heifers.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC38%2FpvVOjtQ%3D%3D&md5=70920ce93051e4b29c64aa2e34dab342CAS | 22127147PubMed |
Malau-Aduli AEO, Siebert BD, Bottema CDK, Pitchford WS (1997) A comparison of the fatty acid composition of triacylglycerols in adipose tissue from Limousin and Jersey cattle. Australian Journal of Agricultural Research 48, 715–722.
| A comparison of the fatty acid composition of triacylglycerols in adipose tissue from Limousin and Jersey cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXktV2jt70%3D&md5=ca850c3f14983413f97bb4c349fb3340CAS |
Moloney AP, Kennedy C, Noci F, Monahan FJ, Kerry JP (2012) Lipid and colour stability of M. longissimus muscle from lambs fed camelina or linseed as oil or seeds. Meat Science 92, 1–7.
| Lipid and colour stability of M. longissimus muscle from lambs fed camelina or linseed as oil or seeds.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xnt1yntL8%3D&md5=96a66e17f55de066dab859b361cd386dCAS | 22516761PubMed |
Morgan JHL, Everitt GC (1969) Some factors affecting yellow fat colour in cattle. Proceedings of the New Zealand Society of Animal Production 29, 164–176.
NRC (2001) ‘Nutrient requirements of dairy cattle.’ 7th revised edn. (National Research Council, National Academy Press: Washington, DC)
Nuernberg K, Dannenberger D, Nuernberg G, Ender K, Voigt J, Scollan ND, Wood JD, Nute GR, Richardson RI (2005) Effect of grass-based and a concentrate feeding system on meat quality characteristics and fatty acid composition of longissimus muscle in different cattle breeds. Livestock Production Science 94, 137–147.
| Effect of grass-based and a concentrate feeding system on meat quality characteristics and fatty acid composition of longissimus muscle in different cattle breeds.Crossref | GoogleScholarGoogle Scholar |
Oliveira RL, Ladeira MM, Barbosa MAAF, Assunção DMP, Matsushita M, Santos GT, Oliveira RL (2008) Linoleic conjugated acid and fatty acids profile in the muscle and fat layer of water buffalo steers fed different fat sources. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 60, 169–178.
| Linoleic conjugated acid and fatty acids profile in the muscle and fat layer of water buffalo steers fed different fat sources.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXnsVyks7Y%3D&md5=f5e4fc5cceb5bb25739ef32fa2511c8bCAS |
Oliveira DM, Ladeira MM, Chizzotti ML, Machado Neto OR, Ramos EM, Gonçalves TM, Bassi MS, Lanna DP, Ribeiro JS (2011) Fatty acid profile and qualitative characteristics of meat from zebu steers fed with different oilseeds. Journal of Animal Science 89, 2546–2555.
| Fatty acid profile and qualitative characteristics of meat from zebu steers fed with different oilseeds.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXps12jtrc%3D&md5=32c955ff0fe26264dd7053288d0a5576CAS | 21383038PubMed |
Oliveira EA, Sampaio AAM, Henrique W, Pivaro TM, Rosa BL, Fernandes ARM, Andrade AT (2012) Quality traits and lipid composition of meat from Nellore young bulls fed with different oils either protected or unprotected from rumen degradation. Meat Science 90, 28–35.
| Quality traits and lipid composition of meat from Nellore young bulls fed with different oils either protected or unprotected from rumen degradation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXht1ens77L&md5=ea7fcf9a3e7648e8908ef85bb892c5beCAS | 21680103PubMed |
Raes K, De Smet S, Demeyer D (2004) Effect of dietary fatty acids on incorporation of long chain polyunsaturated fatty acids and conjugated linoleic acid in lamb, beef and pork meat: a review. Animal Feed Science and Technology 113, 199–221.
| Effect of dietary fatty acids on incorporation of long chain polyunsaturated fatty acids and conjugated linoleic acid in lamb, beef and pork meat: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhtV2ntrc%3D&md5=ee9db4c7216f55331a61e43fc2445706CAS |
San Vito E, Lage JF, Ribeiro AF, Silva RA, Berchielli TT (2015) Fatty acid profile, carcass and quality traits of meat from Nellore young bulls on pasture supplemented with crude glycerin. Meat Science 100, 17–23.
| Fatty acid profile, carcass and quality traits of meat from Nellore young bulls on pasture supplemented with crude glycerin.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhs1Gqt7%2FF&md5=cb01fd9985b4874b8ca98ba690a9e224CAS | 25290759PubMed |
SAS Institute Inc. (2004) ‘SAS/STAT 9.0. User’s guide.’ (SAS: Cary, NC)
Schroeder JW, Cramer DA, Bowling RA, Cook CW (1980) Palatability, shelflife and chemical differences between forage- and grain-finished beef. Journal of Animal Science 50, 852–859.
Scollan N, Hocquette JF, Nuernberg K, Dannenberger D, Richardson I, Moloney A (2006) Innovations in beef production systems that enhance the nutritional and health value of beef lipids and their relationship with meat quality. Meat Science 74, 17–33.
| Innovations in beef production systems that enhance the nutritional and health value of beef lipids and their relationship with meat quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xms1Grsb8%3D&md5=04bf1d5c2222d613a603102fc34a17e1CAS | 22062713PubMed |
Smith T, Thomas MG, Bidner TD, Paschal JC, Franke DE (2009) Single nucleotide polymorphisms in Brahman steers and their association with carcass and tenderness traits. Genetics and Molecular Research 8, 39–46.
| Single nucleotide polymorphisms in Brahman steers and their association with carcass and tenderness traits.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1M7ltlOitg%3D%3D&md5=88ec493da83c135b7e4046c3161603b7CAS | 19224465PubMed |
Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
| Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK38%2FnvVCltA%3D%3D&md5=5ac74045d70ec4fdb1b477d24fb39c1dCAS | 1660498PubMed |
Wood JD, Richardson RI, Nute GR, Fisher AV, Campo MM, Kasapidou E, Sheard PR, Enser M (2004) Effects of fatty acids on meat quality: a review. Meat Science 66, 21–32.
| Effects of fatty acids on meat quality: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXmvVKltrY%3D&md5=1a3530fdb944411fc7be95798b173468CAS | 22063928PubMed |
Wood JD, Enser M, Fisher AV, Nute GR, Sheard PR, Richardson RI, Hughes SI, Whittington FM (2008) Fat deposition, fatty acid composition and meat quality: a review. Meat Science 78, 343–358.
| Fat deposition, fatty acid composition and meat quality: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsVCls7s%3D&md5=b7059b86baab70fe2cf37aa0ec8069c0CAS | 22062452PubMed |
Yang A, Larsen TW, Tume RK (1992) Carotenoid and retinol concentrations in serum, adipose tissue and liver and carotenoid transport in sheep, goats and cattle. Australian Journal of Agricultural Research 43, 1809–1817.
| Carotenoid and retinol concentrations in serum, adipose tissue and liver and carotenoid transport in sheep, goats and cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXktlKg&md5=89077be5591ccf5856b5d36bdcb05f92CAS |
Zinn RA, Gulati SK, Plascencia A, Salinas J (2000) Influence of ruminal biohydrogenation on the feeding value of fat in finishing diets for feedlot cattle. Journal of Animal Science 78, 1738–1746.
