The effect of long term under- and overfeeding on the expression of genes related to glucose metabolism in the mammary tissue of goats
E. Tsiplakou A C , E. Flemetakis B , E. D. Kouri B and G. Zervas A
+ Author Affiliations
- Author Affiliations
A Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Iera Odos 75, GR-11855, Athens, Greece.
B Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, GR-11855, Athens, Greece.
C Corresponding author. Email: eltsiplakou@aua.gr
Animal Production Science 57(4) 622-626 https://doi.org/10.1071/AN15366
Submitted: 10 July 2015 Accepted: 11 December 2015 Published: 20 April 2016
Abstract
The glucose metabolism in the mammary tissue (MT) is controlled by several genes whose nutritional regulation is poorly documented. In this study, the effect of long-term under- and overfeeding on the expression of glucose transporter 1 (GLUT1), glucose transporter 3 (GLUT3), sodium glucose contransporter 1 (SGLT1), two isoforms of β- (1, 4) galactosyltransferase [β- (1, 4) GAT1 and β- (1, 4) GAT3], and α-lactalbumin (LALBA), related to glucose metabolism in goat MT, was examined by using real-time RT-PCR. Twenty- four lactating goats were divided into three homogenous subgroups and fed the same ration in quantities covering 70% (underfeeding), 100% (control) and 130% (overfeeding) of their energy and crude protein requirements, respectively. The results showed that the feeding level did not affect the GLUT1, GLUT3 and SGLT1 genes expression in goats MT. On the contrary, a significant reduction and a trend for reduction on mRNA of β- (1, 4) GAT1 and on β- (1, 4) GAT3, respectively, in the MT of underfed goats, compared with the overfed ones, was observed. Moreover, a significant decrease in the LALBA mRNA accumulation in the MT of underfed goats compared with the overfed was found. In conclusion, the MT of goats, unlike cows, adapt to changes in glucose or energy supply from different levels of feeding by changing the utilisation of glucose for the synthesis of lactose.
Additional keywords: feeding level, mammary gland.
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