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RESEARCH ARTICLE

Nutritive characteristics, mineral concentrations and dietary cation–anion difference of feeds used within grazing-based dairy farms in Australia

Innocent Rugoho A B , Cameron J. P. Gourley A and Murray C. Hannah A
+ Author Affiliations
- Author Affiliations

A Agriculture Research and Development Division, Ellinbank Centre, Department of Economic Development, Jobs, Transport and Resources, Ellinbank, Vic. 3821, Australia.

B Corresponding author. Email: innocent.rugoho@ecodev.vic.gov.au

Animal Production Science 57(5) 858-876 https://doi.org/10.1071/AN15761
Submitted: 30 October 2015  Accepted: 22 February 2016   Published: 14 June 2016

Abstract

Feed types consumed by cows on 44 contrasting dairy farms from all major dairy regions in Australia were identified and sampled on five occasions over a 15-month period to determine nutritive characteristics and mineral concentrations. Perennial ryegrass was the most dominant and commonly used forage, although a broad range of other home-grown forages was also grazed or conserved. Almost all farms utilised cereal or legume grains or grain-based concentrates, as well as a range of by-products and mineral additives. The present study has documented, for the first time, the nutritive characteristics, mineral concentrations and dietary cation–anion difference (DCAD) of the broad variety of feed types used on dairy farms across Australia, and has also highlighted the substantial variation that can occur among and within feed types. While there were effects of season, region and some defined farm-management practices on important nutrients for specific feeds, among-farm variation was generally large. These results suggest that farmers and nutritionists should be mindful when formulating dairy rations using feed nutritive characteristics and mineral concentrations reported in feed databases, as there is a potential for considerable variation in specific feeds. In particular, these defined differences suggest that appropriate feed testing of grazed pasture and conserved forage should be undertaken on at least a seasonal basis to monitor changes in nutritive characteristics and mineral concentrations, so as to optimise dietary formulation for the nutrition of grazing dairy cows.

Additional keywords: by-products, concentrates, conserved forage, DCAD, grazed forage, pasture, silage.


References

AOAC (1990) ‘Association of official analytical chemists. Official methods of analysis.’ (Association of Official Analytical Chemist: Washington, DC)

Auldist MJ, Marett JS, Greenwood JS, Hannah MC, Jacobs JL, Wales WJ (2013) Effects of different strategies for feeding supplements on milk production responses in cows grazing a restricted pasture allowance. Journal of Dairy Science 96, 1218–1231.
Effects of different strategies for feeding supplements on milk production responses in cows grazing a restricted pasture allowance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xhsl2isrrP&md5=ebbf84af267fadd306fa0512c311efc5CAS | 23219117PubMed |

Bramley E, Lean IJ, Fulkerson WJ, Costa ND (2012) Feeding management and feeds on dairy farms in New South wales and Victoria. Animal Production Science 52, 20–29.
Feeding management and feeds on dairy farms in New South wales and Victoria.Crossref | GoogleScholarGoogle Scholar |

Burkitt LL, Small DR, McDonald JW, Wales WJ, Jenkin ML (2007) Comparing irrigated biodynamic and conventionally managed dairy farms. 1. Soil and pasture properties. Australian Journal of Experimental Agriculture 47, 479–488.
Comparing irrigated biodynamic and conventionally managed dairy farms. 1. Soil and pasture properties.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXmt1Cqsrw%3D&md5=a6d0dac5a85ffd184fdad1bd4f309e3dCAS |

Carruthers VR, Neil PG (1997) Milk production and ruminal metabolites from cows offered two pasture diets supplemented with non-structural carbohydrates. New Zealand Journal of Agricultural Research 40, 513–521.
Milk production and ruminal metabolites from cows offered two pasture diets supplemented with non-structural carbohydrates.Crossref | GoogleScholarGoogle Scholar |

Chapman DF, Cullen BR, Johnson IR, Beca D (2009) Interannual variation in pasture growth rate in Australia and New Zealand dairy regions and its consequences for system management. Animal Production Science 49, 1071–1079.
Interannual variation in pasture growth rate in Australia and New Zealand dairy regions and its consequences for system management.Crossref | GoogleScholarGoogle Scholar |

CSIRO (2007) ‘Nutrient requirements of domesticated ruminants.’ (Eds M Freer, H Dove, JV Nolan) (CSIRO Publishing: Melbourne)

Dairy Australia (2010) ‘Feed value varies in different feeds.’ (Dairy Australia: Melbourne) Available at http://www.dairyaustralia.com.au/~/media/Documents/Animal management/Feed and nutrition/Supplementary feeds/G2M Buying feed fact sheet 5-10-05-25.pdf [Verified 30 May 2016]

Dairy Australia (2013) ‘The Australian dairy industry in focus 2013.’ (Dairy Australia: Melbourne) Available at http://www.dairyaustralia.com.au/Industry-information/About-Dairy-Australia/~/media/Documents/Stats and markets/In Focus/Australian Dairy Industry In Focus 2013.pdf [Verified 30 May 2016]

DairyOne (2015) ‘Feed composition libraries.’ Available at http://dairyone.com/analytical-services/feed-and-forage/feed-composition-library/interactive-feed-composition-library/ [Verified 2 February 2015]

Dalley DE, Geddes T (2012) Pasture growth and quality on Southland and Otago dairy farms. Proceedings of the New Zealand Grassland Association 74, 237–241.

de Ruiter J, Dalley DE, Hughes TP, Fraser TJ, Dewhurst RJ (2007) Types of supplements: their nutritive value and use. In ‘Pasture and supplements for grazing animals’. (Eds PV Rattray, IM Brrokes, AM Nicol) pp. 97–115. (New Zealand Society of Animal Production: Christchurch, New Zealand)

Delagarde R, Peyraud JL, Delay L, Faverdin P (2000) Vertical distribution of biomass, chemical composition and pepsin-cellulase digestibility in a perennial ryegrass sward: interaction with month of year, regrowth age and time of day. Animal Feed Science and Technology 84, 49–68.
Vertical distribution of biomass, chemical composition and pepsin-cellulase digestibility in a perennial ryegrass sward: interaction with month of year, regrowth age and time of day.Crossref | GoogleScholarGoogle Scholar |

Dharma S, Shafron W, Oliver M (2012) ‘Australian dairy: farm technology and management practices 2010–11.’ (Australian Bureau of Agricultural and Resource Economics and Sciences: Canberra)

Doyle PT, Stockdale CR, Lawson AR, Cohen DC (2000) ‘Pastures for dairy production in Victoria.’ 2nd edn. (The State of Victoria, Department of Natural Resources and Environment: Tatura, Vic.)

Fariña SR, Garcia SA, Fulkerson WJ (2011) A complementary forage system whole-farm study: forage utilisation and milk production. Animal Production Science 51, 460–470.
A complementary forage system whole-farm study: forage utilisation and milk production.Crossref | GoogleScholarGoogle Scholar |

Ferris CP (2007) Sustainable pasture-based systems: meeting the challenges. Canadian Journal of Plant Science 87, 723–738.
Sustainable pasture-based systems: meeting the challenges.Crossref | GoogleScholarGoogle Scholar |

Finneran E, Crosson P, Shalloo L, Foristal D, O’ Kiely P, Wallace M (2010) Simulation modelling of the cost of producing and utilising feeds for ruminats on Irish farms. Journal of Farm Management 14, 95–116.

Fulkerson WJ, Slack K, Hennessy DW, Hough GM (1998) Nutrients in ryegrass (Lolium spp.), white clover (Trifolium repens) and Kikuyu (Pennisetum clandestinum) pastures in relation to season and stage of regrowth in a subtropical environment. Australian Journal of Experimental Agriculture 38, 227–240.
Nutrients in ryegrass (Lolium spp.), white clover (Trifolium repens) and Kikuyu (Pennisetum clandestinum) pastures in relation to season and stage of regrowth in a subtropical environment.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXksVyhuro%3D&md5=c3c788d81771a2b8d5adee977bbfb382CAS |

Fulkerson WJ, Neal JS, Clark CF, Horadagoda A, Nandra KS, Barchia I (2007) Nutritive value of forage species grown in the warm tempatate climate of Australia for dairy cows: grasses and legumes. Livestock Science 107, 253–264.
Nutritive value of forage species grown in the warm tempatate climate of Australia for dairy cows: grasses and legumes.Crossref | GoogleScholarGoogle Scholar |

Gourley CJP, Dougherty WJ, Weaver DM, Aarons SH, Awty IM, Gibson DM, Hannah MC, Smith AP, Peverill KI (2012) Farm-scale nitrogen, phosphorus, potassium and sulphur balances and use efficiences on Australian dairy farms. Animal Production Science 52, 929–944.
Farm-scale nitrogen, phosphorus, potassium and sulphur balances and use efficiences on Australian dairy farms.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xht1GrsL7O&md5=f530dd6a35ddccaccac8019e84e6f2b4CAS |

Hall MB, Mertens DR (2012) A ring test of in vitro neutral detergent fiber digestibility: analytical variability and sample ranking. Journal of Dairy Science 95, 1992–2003.
A ring test of in vitro neutral detergent fiber digestibility: analytical variability and sample ranking.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XkslaqsL0%3D&md5=1879faf4d4c85cb49e470b75f177a1d5CAS | 22459845PubMed |

Heard JW, Doyle PT, Francis SA, Stains MVH, Wales WJ (2011) Calculating dry matter consumption of dairy herds in Australia: the need to fully account for energy requirements and issues with estimating energy supply. Animal Production Science 51, 605–614.
Calculating dry matter consumption of dairy herds in Australia: the need to fully account for energy requirements and issues with estimating energy supply.Crossref | GoogleScholarGoogle Scholar |

Hennessy DW, McLennan DJ, Williamson PJ, Morris SG (1998) Changes in characteristics of pastures in the coastal subtropics when grazed by cattle during years of rainfall. Australian Journal of Experimental Agriculture 38, 813–820.
Changes in characteristics of pastures in the coastal subtropics when grazed by cattle during years of rainfall.Crossref | GoogleScholarGoogle Scholar |

Higgs RJ, Chase LE, Ross DA, Van Amburgh ME (2015) Updating the Cornell net carbohydrate and protein system feed library and analysing model sentivity to feed inputs. Journal of Dairy Science 98, 6340–6360.
Updating the Cornell net carbohydrate and protein system feed library and analysing model sentivity to feed inputs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhtFehs7fE&md5=c625f8d422da65cab6d90ec862863265CAS | 26142848PubMed |

Holmes CW (1989) ‘Grass: its production and utilisation.’ 2nd edn. (Blackwell Scientific Publications: London)

Holmes CW, Wilson GF (1987) ‘Milk production from pasture.’ 2nd edn. (Butterworths: Wellington, New Zealand)

Hopkins A, Wilkins RJ (2006) Temperate grassland: key developments in the last century and future perspectives. Journal of Agricultural Science, Cambridge 144, 503–523.
Temperate grassland: key developments in the last century and future perspectives.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht1Cls7fO&md5=303a81a57c6f149f77e363cb3325356cCAS |

Hunt MG, Rasmussen S, Newton PCD, Parsons AJ, Newman JA (2005) Near-term impacts of elevated CO2, nitrogen and fungal endophyte-infection on Lolium perenne L. growth, chemical composition and alkaloid production. Plant, Cell & Environment 28, 1345–1354.
Near-term impacts of elevated CO2, nitrogen and fungal endophyte-infection on Lolium perenne L. growth, chemical composition and alkaloid production.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXht1Olu73O&md5=38ca3a5fa3171abefeebe7eeb17599dcCAS |

Jacobs JL (2014) Challenges in ration formulation in pasture-based milk production systems. Animal Production Science 54, 1130–1140.
Challenges in ration formulation in pasture-based milk production systems.Crossref | GoogleScholarGoogle Scholar |

Jacobs JL, McKenzie FR (2003) ‘A target-driven approach to increase forage production and utilisation in southern dairy systems.’ (Department of Primary Industries: Warnambool, Vic.)

Jacobs JL, Rigby SE (1999) ‘Minerals in dairy pastures in Victoria.’ (Department of Natural Resources and Environment: Warrnambool, Vic.)

Jacobs JL, Ward GN (2011) Effect of nitrogen application on dry matter yields, nutritive characteristics and mineral content of summer-active forage crops in southern Australia. Animal Production Science 51, 77–86.
Effect of nitrogen application on dry matter yields, nutritive characteristics and mineral content of summer-active forage crops in southern Australia.Crossref | GoogleScholarGoogle Scholar |

Jacobs JL, McKenzie FR, Rigby SE, Kearney G (1998) Effect of nitrogen fertiliser aplication and length of lock up on dairy pasture dry matter yield and quality for silage in south-western Victoria. Australian Journal of Experimental Agriculture 38, 219–226.
Effect of nitrogen fertiliser aplication and length of lock up on dairy pasture dry matter yield and quality for silage in south-western Victoria.Crossref | GoogleScholarGoogle Scholar |

Jacobs JL, McKenzie FR, Ward GN (1999) Changes in the botanical composition and nutritive characteristics of pasture, and nutrient selection by dairy cows grazing rainfed pastures in western Victoria. Australian Journal of Experimental Agriculture 39, 419–428.
Changes in the botanical composition and nutritive characteristics of pasture, and nutrient selection by dairy cows grazing rainfed pastures in western Victoria.Crossref | GoogleScholarGoogle Scholar |

Jones GB, Tracy BF (2015) Evaluating seasonal variation in mineral concentration of cool-season pasture herbage. Grass and Forage Science 70, 94–101.
Evaluating seasonal variation in mineral concentration of cool-season pasture herbage.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhslamt7o%3D&md5=66e64dab95b26bb0bb0fa80daa593878CAS |

Kellaway RC, Porta S (1993) ‘Feeding concentrates: supplements for dairy cows.’ (Ed. R Hopkins) (Dairy Research and Development Corporation Publication: Melbourne)

Kertz AF (1998) Variability in delivery of nutrients to lactating dairy cows. Journal of Dairy Science 81, 3075–3084.
Variability in delivery of nutrients to lactating dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXnsV2jtb0%3D&md5=416c4edd5ae9308b0b551b6889fe74e7CAS | 9839247PubMed |

Kolver ES, Muller LD (1998) Performance and nutrient intake of high producing Holstein cows consuming pasture or a total mixed ration. Journal of Dairy Science 81, 1403–1411.
Performance and nutrient intake of high producing Holstein cows consuming pasture or a total mixed ration.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXjsVyjsrs%3D&md5=b4c65ecf09ce8ae3c5cd9cc17b4b38c5CAS | 9621244PubMed |

Lean I, DeGaris P (2010) ‘Transition cow management. A review for nutritional professionals, veterinarians and farm advisers.’ (Eds S Little, J Penry) (Dairy Australia: Melbourne) Available at http://www.dairyaustralia.com.au/~/media/Documents/Animal management/Feed and nutrition/Nutrition management/G2M – InCalf transition cow mgt review – low res.pdf [Verified 30 May 2016]

Litherland AJ, Lambert MG (2007) Factors affecting the quality of pastures and supplements produced on farms. In ‘Pastures and supplements for grazing livestock’. (Eds PV Rattray, IM Brrokes, AM Nicol) pp. 81–96. (New Zealand Society of Animal Production: Christchurch, New Zealand)

McKenzie FR, Jacobs JL, Kearney G (2003) Long-term effects of multiple applications of nitrogen fertiliser on grazed dryland perennial ryegrass/white clover dairy pastures in south-west Victoria. 3. Botanical composition, nutritive characteristics, mineral content, and nutrient selection. Australian Journal of Agricultural Research 54, 477–485.
Long-term effects of multiple applications of nitrogen fertiliser on grazed dryland perennial ryegrass/white clover dairy pastures in south-west Victoria. 3. Botanical composition, nutritive characteristics, mineral content, and nutrient selection.Crossref | GoogleScholarGoogle Scholar |

Metson AJ, Saunders MH (1978) Seasonal variation in chemical composition of pasture. I. Calcium, magnesium, potassium, sodium and phosphorus. New Zealand Journal of Agricultural Research 21, 341–353.
Seasonal variation in chemical composition of pasture. I. Calcium, magnesium, potassium, sodium and phosphorus.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE1cXlvFersLo%3D&md5=69583954583ea33b783a90954bb59af0CAS |

Moller S, Edwards NJ, Parker WJ, Hodgson J, Wilson GF (1996) Nitrogen application to dairy pasture- the effect of the rate and timing of spring nitrogen applications on the concentration of pasture nutrients. Proceedings of the New Zealand Society of Animal Production 56, 276–279.

NANP (2015) ‘Feed and ingredient information.’ Available at http://nanp-nrsp-9.org/feed/search/?animal=all&feed [Accessed 2 February 2015]

NATA (2004) ‘Guide to Nata proficiency testing, 2004, Version 1.’ (National Association of Testing Authorities: Sydney)

Neal M, Neal J, Fulkerson WJ (2007) Optimal choice of dairy forages in eastern Australia. Journal of Dairy Science 90, 3044–3059.
Optimal choice of dairy forages in eastern Australia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXlvFOitbs%3D&md5=83b7f94ad44a349b5ceba558782ae818CAS | 17517747PubMed |

Nobilly FH, McKenzie BA, Edwards GR (2013) Productivity of rotationally grazed simple and diverse pasture mixtures under irrigation in Canterbury. Proceedings of the New Zealand Grassland Association 75, 165–172.

Ostrowski-Meissner HT (1987) ‘Australian feed composition tables. National Collection (1970–1987)’ (AFIC–CSIRO Publication: Sydney)

Parsons AJ, Rasmussen S, Xue H, Newman JA, Anderson CB, Cosgrove GP (2004) Some ‘high sugar grasses’ don’t like it hot. Proceedings of the New Zealand Grassland Association 66, 265–271.

Pembleton KG, Tozer KN, Edwards GR, Jacobs JL, Turner LR (2014) Simple versus diverse pastures: opportunities and challenges in dairy systems. In ‘Proceedings of the 6th Australasian dairy science symposium 2014’. (Ed. JR Roche) pp. 206–216. (The Australasian Dairy Science committee: Hamilton, New Zealand)

Penno JW, Bryant RH, Carter WA, Macdonald KA (2006) Responses to supplementation by dairy cows given low pasture allowances in different seasons 1. Pasture intake and substitution. Animal Science 82, 661–670.
Responses to supplementation by dairy cows given low pasture allowances in different seasons 1. Pasture intake and substitution.Crossref | GoogleScholarGoogle Scholar |

PWC (2011) ‘The Australian dairy industry: the basics.’ (PricewaterhouseCoopers: Sydney) Available at http://www.pwc.com.au/industry/agribusiness/assets/Australian-Dairy-Industry-Nov11.pdf [Verified 30 May 2016]

Reeves M, Fulkerson WJ, Kellaway RC (1996) Forage quality of kikuyu (Pennisetum clandestinum): the effect of time of defoliation and nitrogen fertiliser application and incomparison with perennial ryegrass (Lolium perenne). Australian Journal of Agricultural Research 47, 1349–1359.
Forage quality of kikuyu (Pennisetum clandestinum): the effect of time of defoliation and nitrogen fertiliser application and incomparison with perennial ryegrass (Lolium perenne).Crossref | GoogleScholarGoogle Scholar |

Roche JR, Dalley DE, Moate P, Grainger C, Hannah M, O’Mara F, Rath M (2000) Variations in the dietary cation-anion difference and the acid-base balance of dairy cows in a pasture-based diet in south-eastern Australia. Grass and Forage Science 55, 26–36.
Variations in the dietary cation-anion difference and the acid-base balance of dairy cows in a pasture-based diet in south-eastern Australia.Crossref | GoogleScholarGoogle Scholar |

Roche JR, Turner LR, Lee JM, Edmeades DC, Donaghy DJ, McDonald KA, Penno JW, Berry DP (2009) Weather, herbage quality and milk production in pastoral systems. 3. Inter-relationships and associations between weather variables and herbage growth rate, quality and mineral concentration. Animal Production Science 49, 211–221.
Weather, herbage quality and milk production in pastoral systems. 3. Inter-relationships and associations between weather variables and herbage growth rate, quality and mineral concentration.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXisFSnt74%3D&md5=21892b1f0254e2a434a49effd7be3f29CAS |

Rugoho I, Liu Y, Dewhurst RJ (2014) Analysis of major fatty acids in milk produced from high-quality grazed pasture. New Zealand Journal of Agricultural Research 57, 165–179.
Analysis of major fatty acids in milk produced from high-quality grazed pasture.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhtF2qtL7I&md5=958c4af7546a822b9f4fffb1df480ea9CAS |

Scott BA, Camacho A, Golder H, Molfino J, Kerrisk KL, Lean I, Garcia SC, Chaves AV, Hall E, Clark CEF (2014) The nutritive value of pasture ingested by dairy cows varies within a herd. In ‘Proceedings of the 6th Australasian dairy science symposium 2014’. (Ed. JR Roche) pp. 343–346. (The Australasian Dairy Science committee: Hamilton, New Zealand)

St-Pierre NR, Weiss WP (2015) Partitioning variation in nutrient composition data of common feeds and mixed diets on commercial dairy farms. Journal of Dairy Science 98, 5004–5015.
Partitioning variation in nutrient composition data of common feeds and mixed diets on commercial dairy farms.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXotlKjsLY%3D&md5=ca0c02c757643294d2ea5fe0ac631933CAS | 25981080PubMed |

Stergiadis S, Allen M, Chen XJ, Wills D, Yan T (2015) Prediction of nutrient digestibility and energy concentrations in fresh grass using nutrient composition. Journal of Dairy Science 98, 3257–3273.
Prediction of nutrient digestibility and energy concentrations in fresh grass using nutrient composition.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXjvFOiu7Y%3D&md5=fd76e9e916f2aa16a370fe8f96e108acCAS | 25747838PubMed |

Stockdale CR (1983) The growth and utilisation of irrigated pastures grazed by dairy cows. MSc Thesis, University of Melbourne, Australia.

Stockdale CR, Cohen DC, Doyle PT (2001) Nutritive characteristics of irrigated perennial pastures in northern Victoria and the selection on nutrients by grazing dairy cows. Australian Journal of Experimental Agriculture 41, 601–609.
Nutritive characteristics of irrigated perennial pastures in northern Victoria and the selection on nutrients by grazing dairy cows.Crossref | GoogleScholarGoogle Scholar |

Unkovich M (2012) Nitrogen fixation in Australian systems: review and prospect. Crop and Pasture Science 63, 787–804.
Nitrogen fixation in Australian systems: review and prospect.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhvVSkt7jN&md5=c8a9d2ab01dce7a7961be75f363fe3e8CAS |

van Vuuren AM, Tamminga S, Ketelaar RS (1991) In sacco degradation of organic matter and crude protein of fresh grass (Lolium perenne) in the rumen of grazing dairy cows. Journal Of Agricultural Science, Cambridge 116, 429–436.
In sacco degradation of organic matter and crude protein of fresh grass (Lolium perenne) in the rumen of grazing dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXmtlWisrs%3D&md5=e6ce5e1ab74ac0f86a95b5e56a0b99d4CAS |

VSN International (2013) ‘Genstat for Windows.’ 16th edn. (VSN International: Hemel Hempstead, UK)

VSN International (2014) ‘Genstat for Windows.’ 17th edn. (VSN International: Hemel Hempstead, UK)

Whitehead DC, Barnes RJ, Jones LHP (1983) Nitrogen, sulphur and other mineral elements in white clover and perennial ryegrass grown in mixed swards, with and without fertiliser N at a range of sites in the UK. Journal of the Science of Food and Agriculture 34, 901–909.
Nitrogen, sulphur and other mineral elements in white clover and perennial ryegrass grown in mixed swards, with and without fertiliser N at a range of sites in the UK.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3sXlsFOhu7c%3D&md5=27474639abf8f802cbd5bec61c682b15CAS |

Wilman D, Acuña PGH, Michaud PJ (1994) Concentrations of N, P, K, Ca, Mg and Na in perennial ryegrass and white clover leaves of different ages. Grass and Forage Science 49, 422–428.
Concentrations of N, P, K, Ca, Mg and Na in perennial ryegrass and white clover leaves of different ages.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXltlGjuro%3D&md5=e6f84f895c412d832b0a33c868661903CAS |

Wilson GF, Moller S, Parker WJ, Hodgson J (1995) Seasonal differences in pasture composition and nutritional implications. Dairy Farming Annual 47, 46–56.