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RESEARCH ARTICLE

Disease resistance genes in a doubled haploid population of two-rowed barley segregating for malting quality attributes

L. C. Emebiri A C , G. Platz B and D. B. Moody A
+ Author Affiliations
- Author Affiliations

A Department of Primary Industries, Grains Innovation Park, Natimuk Road, Private Bag 260, Horsham, Vic. 3401, Australia.

B Department of Primary Industries, Hermitage Research Station, 604 Yangan Road, Warwick, Qld 4370, Australia.

C Corresponding author. Email: Livinus.Emebiri@dpi.vic.gov.au

Australian Journal of Agricultural Research 56(1) 49-56 https://doi.org/10.1071/AR04102
Submitted: 10 May 2004  Accepted: 29 November 2004   Published: 31 January 2005

Abstract

Malting barley (Hordeum vulgare L.) improvement involves selection for many quality traits, but the search for and deployment of resistance genes has continued to be an equally important endeavour. As an aid to phenotypic selection in breeding programs, gene mapping can serve to characterise genes known to exist in elite breeding lines. In the present study, 180 doubled haploid lines derived from the cross of VB9524/ND11231*12 were screened for disease resistance under field and greenhouse conditions. Quantitative trait locus (QTL) mapping and classical genetic linkage approaches were used to identify and map QTLs for resistance to powdery mildew (Blumeria graminis f.sp. hordei), net form of net blotch (Pyrenophora teres f. teres) and stem rust (Puccinia graminis f.sp. tritici). The analyses offered a comparison between QTL mapping and traditional genetic linkage analysis. Both approaches identified a QTL for powdery mildew resistance on chromosome 1H, which mapped to the approximate genomic location of the Mla6 gene. Similarly, both methods identified a major QTL for resistance to net form of net blotch on chromosome 6H and for stem rust resistance on chromosome 7H. The QTL for stem rust resistance on 7H mapped to the approximate location of the Rpg1 gene. Classical linkage analysis identified the 3 QTLs with major effects, but was unable to detect 3 other loci with minor effects.

Additional keywords: two-row barley, molecular markers, QTL, linkage analysis, powdery mildew (Blumeria graminis f.sp. hordei), stem rust (Puccinia graminis f.sp. tritici), net form of net blotch (Pyrenophora teres f. teres).


Acknowledgments

This work was supported by grants from the Grains Research and Development Corporation (GRDC), Australia. We thank members of the Australian Barley Molecular Marker Project (ABMMP) for invaluable contributions to the study.


References


Backes G, Madsen LH, Jaiser H, Stougaard H, Herz M, Mohler V, Jahoor A (2003) Localisation of genes for resistance against Blumeria graminis f.sp. hordei and Puccinia graminis in a cross between a barley cultivar and a wild barley (Hordeum vulgare ssp. spontaneum) line. Theoretical and Applied Genetics 106, 353–362.
PubMed |
open url image1

Borovkova IG, Steffenson BJ, Jin Y, Rasmussen JB, Kilian A, Kleinhofs A, Rossnagel BG, Kao KN (1995) Identification of molecular markers linked to the stem rust resistance gene rpg4 in barley. Phytopathology 85, 181–185. open url image1

Brueggeman R, Rostoks N, Kudrna D, Kilian A, Han F, Chen J, Druka A, Steffenson B, Kleinhofs A (2002) The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases. Proceedings of the National Academy of Sciences of the United States of America 99, 9328–9333.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Cakir M, Gupta S, Platz GJ, Ablett GA, Loughman R , et al. (2003) Mapping and validation of the genes for resistance to Pyrenophora teres f. teres in barley (Hordeum vulgare L.). Australian Journal of Agricultural Research 54, 1369–1377.
Crossref | GoogleScholarGoogle Scholar | open url image1

Chelkowski J, Tyrka M, Sobkiewiez A (2003) Resistance genes in barley and their identification with molecular markers. Journal of Applied Genetics 44, 291–309.
PubMed |
open url image1

Emebiri LC, Moody DB, Panozzo JF, Chalmers KJ, Kretschmer JM, Ablett GA (2003) Identification of QTLs associated with variations in grain protein concentration in two-row barley. Australian Journal of Agricultural Research 54, 1211–1221.
Crossref | GoogleScholarGoogle Scholar | open url image1

Emebiri LC, Moody DB, Panozzo JF, Read BJ (2004) Mapping of QTLs for malting quality attributes in barley based on a cross of parents with low grain protein concentration. Field Crops Research 87, 195–205.
Crossref | GoogleScholarGoogle Scholar | open url image1

Fox SL, Harder DE (1995) Resistance to stem rust in barley and inheritance of resistance to race QCC. Canadian Journal of Plant Science 75, 781–788. open url image1

Franckowiak JD (1996) Revised linkage maps for morphological markers in barley, Hordeum vulgare. Special Issue Barley Genetics Newsletter 26, 9–21. open url image1

Horvath H, Rostoks N, Brueggeman R, Steffenson B, Wettstein von D, Kleinhofs A (2003) Genetically engineered stem rust resistance in barley using the Rpg1 gene. Proceedings of the National Academy of Sciences of the United States of America 100, 364–369.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Jansen RC, Stam P (1994) High resolution of quantitative traits into multiple loci via interval mapping. Genetics 136, 1447–1455.
PubMed |
open url image1

Kilian A, Steffenson BJ, Maroof MAS, Kleinhofs A (1994) RFLP markers linked to the durable stem rust resistance gene Rpg1 in barley. Molecular Plant-Microbe Interactions 7, 298–301.
PubMed |
open url image1

Kleinhofs A (2004) Integrating molecular and morphological/physiological marker maps. Barley Genetics Newsletter 34, 14–25. open url image1

Lander ES, Schork NJ (1994) Genetic dissection of complex traits. Science 265, 2037–2048.
PubMed |
open url image1

Ma ZQ, Lapitan NLV, Steffensen B (2004) QTL mapping of net blotch resistance genes in a doubled-haploid population of six-rowed barley. Euphytica 137, 291–296.
Crossref | GoogleScholarGoogle Scholar | open url image1

Manninen O, Kalendar R, Robinson J, Schulman AH (2000) Application of BARE-1 retrotransposon markers to the mapping of a major resistance gene for net blotch in barley. Molecular and General Genetics 264, 325–334.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Raman H, Platz GJ, Chalmers KJ, Raman R, Read BJ, Barr AR, Moody DB (2003) Mapping of genomic regions associated with net form of net blotch resistance in barley. Australian Journal of Agricultural Research 54, 1359–1367.
Crossref | GoogleScholarGoogle Scholar | open url image1

Richter K, Schondelmaier J, Jung C (1998) Mapping of quantitative trait loci affecting Drechslera teres resistance in barley with molecular markers. Theoretical and Applied Genetics 97, 1225–1234.
Crossref | GoogleScholarGoogle Scholar | open url image1

Sato K, Takeda K, Hayes PM (1996) QTL analysis for net blotch resistance in barley. ‘Proceedings of the V International Oat Conference & VII International Barley Genetics Symposium’. Poster Sessions Vol. 1. (Ed. A. Slinkard ) pp. 298–300. (University Extension Press, University of Saskatchewan: Saskatoon, Canada)


Spaner D, Shugar LP, Chao TM, Falak I, Briggs KG , et al. (1998) Mapping of disease resistance loci in barley on the basis of visual assessment of naturally occurring symptoms. Crop Science 38, 843–850. open url image1

Steffenson BJ, Hayes PM, Kleinhofs A (1996) Genetics of seedling and adult plant resistance to net blotch (Pyrenophora teres f. teres) and spot blotch (Cochliobolus sativus) in barley. Theoretical and Applied Genetics 92, 552–558.
Crossref | GoogleScholarGoogle Scholar | open url image1

Tanksley SD (1993) Mapping polygenes. Annual Review of Genetics 27, 205–233.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Utz HF, Melchinger AE (1996) PLABQTL: a program for composite interval mapping of QTL. Journal of Agricultural Genomics , open url image1

Utz HF, Melchinger AE, Schon CC (2000) Bias and sampling error of the estimated proportion of genotypic variance explained by quantitative trait loci determined from experimental data in maize using cross validation and validation with independent samples. Genetics , 1839–1849. open url image1

Voorrips RE (2002) MapChart: Software for the graphical presentation of linkage maps and QTLs. Journal of Heredity 93, 77–78.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Vowden CJ, Ridout MS, Tobutt KR (1995) LINKEM: a program for genetic linkage analysis. Journal of Heredity 86, 249–250. open url image1

Williams KJ (2003) The molecular genetics of disease resistance in barley. Australian Journal of Agricultural Research 54, 1065–1079.
Crossref | GoogleScholarGoogle Scholar | open url image1

Zeng Z-B (1994) Precision mapping of quantitative trait loci. Genetics 136, 1457–1468.
PubMed |
open url image1