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RESEARCH ARTICLE

Genetic variation in populations of Western Australian wild radish

P. Madhou A , A. Wells B , E. C. K. Pang A and T. W. Stevenson A C
+ Author Affiliations
- Author Affiliations

A RMIT University, School of Applied Sciences, Plenty Road, Bundoora, Vic. 3083, Australia.

B Nufarm Limited, 103-105, Pipe Road, Laverton North, Vic. 3026, Australia.

C Corresponding author. Email: trevor@rmit.edu.au

Australian Journal of Agricultural Research 56(10) 1079-1087 https://doi.org/10.1071/AR04265
Submitted: 4 November 2004  Accepted: 4 August 2005   Published: 25 October 2005

Abstract

Raphanus raphanistrum L. (wild radish) is a major problematic weed worldwide. Random amplified polymorphic DNA (RAPD) was used to estimate the degree of genetic diversity between and within 2 populations of wild radish (WARR 5 and WARR 6), found to exhibit multiple herbicide resistance compared with a susceptible population (WARR 7). It is believed that weed species with high degrees of genetic variation show potential for developing resistance to herbicides. Of the 13 RAPD primers screened, 9 primers generated 97 polymorphic bands concomitant with a high level of polymorphism (82%) between the wild radish populations, characteristics of an outbreeding species. Analysis of molecular variance (AMOVA) showed a markedly higher proportion of diversity within populations (87%) as opposed to between populations (13%). Principal component analysis (PCA) further highlighted the large amount of variation between individuals within populations. Only one marker, OPC19–8, was found to be unique to the WARR 7 population but absent in WARR 5 and in most individuals of the WARR 6 populations. This marker may potentially be correlated with herbicide susceptibility. The 2 resistant wild radish populations were found to be closely related (0.7% dissimilar) to each other, whereas the susceptible population was genetically dissimilar to them by 2.3%. This higher level of dissimilarity between the susceptible and resistant populations may be explained by limited gene flow between them since the susceptible population is geographically located further away from the resistant populations. Hence, it may be concluded that the underlying genetic structure of the 3 wild radish populations seems to be similar despite WARR 6 and WARR 5 having been exposed to mixed herbicide usage for over 17 years.

Additional keywords: Raphanus raphanistrum, RAPD, genetic diversity.


Acknowledgments

The authors are grateful to Michael Walsh for his help in providing the wild radish seeds and the background information. This work was supported by Nufarm Australia Limited.


References


Cheam, AH ,  and  Code, GR (1998). ‘ L. Biology of Australian weeds.’ (R.G. and F.J. Richardson: Melbourne, Vic.)

Code GR, Reeves TG (1981) Chemical control of wild radish in wheat. ‘Proceedings of the 6th Australian Weeds Conference’. Vol 1. (The Weed Society of Queensland: Gold Coast, Qld)


Darmency H (1994) Genetics of herbicide resistance in weeds and crops. ‘Herbicide resistance in plants: biology and biochemistry’. (Eds S Powles, J Holtum) pp. 263–297. (Lewis Publishers: Boca Raton, FL)

Donaldson TW (1986) Wild radish (Raphanus raphanistrum L.): a review of research on its biology and control in Victoria, 1976–1982. Plant Protection Quarterly 1, 160–162. open url image1

Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction sites. Genetics 131, 647–656. open url image1

de Gruijter JM, Ziem J, Verweij JJ, Polderman AM, Gasser RB (2004) Genetic substructuring within Oesophagostomum bifurcum (Nematoda) from human and non-human primates from Ghana based on random amplified polymorphic DNA analysis. The American Journal of Tropical Medicine and Hygiene 71, 227–233.
PubMed |
open url image1

Holt RD, Hochberg ME (1997) When is biological control evolutionarily stable (or is it)? Ecology 78, 1673–1683. open url image1

Huh MK, Ohnishi O (2001) Allozyme diversity and population structure of Japanese and Korean populations of wild radish, Raphanus sativus var. hortensis f. raphanistroides (Brassicaceae). Genes and Genetic Systems 76, 15–23.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Huh MK, Ohnishi O (2002) Genetic diversity and genetic relationships of East Asia natural populations of wild radish revealed by AFLP. Breeding Science 52, 79–88.
Crossref | GoogleScholarGoogle Scholar | open url image1

Jasieniuk M, Brule-Babel AL, Morrison IN (1996) The evolution and genetics of herbicide resistance in weeds. Weed Science 44, 176–193. open url image1

Kim KS, Sappington TW (2004) Genetic structuring of boll weevil populations in the US based on RAPD markers. Insect Molecular Biology 13, 293–303.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Lougheed SC, Gibbs HL, Prior KA, Weatherhead PJ (2000) A comparison of RAPD versus microsatellite DNA markers in population studies of the massasauga rattlesnake. The American Genetic Association 91, 458–463. open url image1

Meikle A, Finch RP, McRoberts N, Marshall G (1999) A molecular genetic assessment of herbicide-resistant Sinapsis arvensis.  Weed Research 39, 149–158.
Crossref | GoogleScholarGoogle Scholar | open url image1

Moodie M, Finch RP, Marshall G (1997) Analysis of genetic variation in wild mustard (Sinapsis arvensis) using molecular markers. Weed Science 45, 102–107. open url image1

Nei M (1972) Genetic distance between populations. American Naturalist 106, 283–292.
Crossref | GoogleScholarGoogle Scholar | open url image1

Nei M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America 70, 3321–3323.
PubMed |
open url image1

Piggin CM, Reeves TG, Brooke HD, Code GR (1978) Germination of wild radish (Raphanus raphanistrum L.). ‘Proceedings 1st Conference of the Council of Australian Weed Science Societies’. (Council of Australian Weed Science Societies: Parkville, Vi.c)


SPSS Inc. (1998). ‘SPSS Base 8.0 for Windows user’s guide.’ (SPSS Inc.: Chicago, IL)

StatSoft Inc. (1995). ‘STATISTICA for Windows [Computer program manual].’ (StatSoft, Inc.: Tulsa, OK)

Walsh MJ, Powles SB, Beard BR, Parkin BT, Porter SA (2004) Multiple-herbicide resistance across four modes of action in wild radish (Raphanus raphanistrum). Weed Science 52, 8–13. open url image1

Yeh, FC , Yang, RC ,  and  Boyle, T (1999). ‘POPGENE, Version 1.31. Microsoft Windows-based software for population genetics analysis.’ (University of Alberta and Centre for International Forestry Research: Alberta, Canada)