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Australian Journal of Botany Australian Journal of Botany Society
Southern hemisphere botanical ecosystems
RESEARCH ARTICLE

Essential and toxic element concentrations in Hypericum perforatum

Dolja Pavlova A D , Irina Karadjova B and Ilina Krasteva C
+ Author Affiliations
- Author Affiliations

A Department of Botany, Faculty of Biology, University of Sofia, blvd. Dragan Tzankov 8, 1164 Sofia, Bulgaria.

B Department of Analytical Chemistry, Faculty of Chemistry and Pharmacy, University of Sofia, blvd. James Bouchier 1, 1164 Sofia, Bulgaria.

C Department of Pharmacognosy, Faculty of Pharmacy, Medical University, 2 Dunav str., 1000 Sofia, Bulgaria.

D Corresponding author. Email: pavlova@biofac.uni-sofia.bg

Australian Journal of Botany 63(2) 152-158 https://doi.org/10.1071/BT14260
Submitted: 12 October 2014  Accepted: 5 March 2015   Published: 20 April 2015

Abstract

The herb Hypericum perforatum L. – otherwise known as St John’s Wort – that grows on serpentine and non-serpentine sites is widely used in traditional medicine in Bulgaria. Plant aerial parts (Hiperici herba) as well as methanol/water (1 : 1) and water extracts prepared from eight populations growing on and off serpentine were analysed for essential (Fe, Mn, Cr, Co, Cu, Zn) and toxic (Ni, Cd, Pb) elemental concentration using inductively plasma optical emission spectrometry and electro thermal atomic absorption spectrometry. The results showed that metal concentrations varied across sites. Non-serpentine populations were well separated based on their total metal concentrations. The plant is Ni tolerant, but has the potential to accumulate trace elements at toxic levels. The transfer of elements from the herb material to an extract was solvent and metal dependent. The highest mobility, e.g. percentages of extraction, was found for Cu, Zn and Ni in all extracts. The amount of Cd, Ni and Cr was above the permissible limits in dry plant material and both types of extracts. The element concentrations found in this medicinal plant show that people should be careful when collecting it from serpentine sites and using it for medication.

Additional keywords: extracts, metals, herb, pharmaceutical botany, plant–soil relations, St John’s wort.


References

Akula R, Ravishankar GA (2011) Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling & Behavior 6, 1720–1731.
Influence of abiotic stress signals on secondary metabolites in plants.Crossref | GoogleScholarGoogle Scholar |

Antić-Mladenović BS, Radanović SD (2012) Factors affecting heavy metals content in medicinal and aromatic plants and related pharmaceutical products. In ‘Proceedings of the seventh conference on medicinal and aromatic plants of southeast European countries (Proceedings of the 7th CMAPSEEC), Serbia’. (Eds Z Stevanović, D Radanović) pp. 226–228. (Institute for Medicinal Plant Research ‘Dr Josif Pancic’: Belgrade and Association for Medicinal and Aromatic Plants of Southeast European Countries (AMAPSEEC): Belgrade)

Ayan AK, Kizilkaya R, Cirak C, Kevseroglu K (2006) Heavy metal content of St John’s wort (Hypericum perforatum L.) growing in Northern Turkey. Journal of Plant Sciences 1, 182–186.
Heavy metal content of St John’s wort (Hypericum perforatum L.) growing in Northern Turkey.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xpslagsb0%3D&md5=4e22eb335d3870ac11f39084841805fcCAS |

Bağdat RB, Eid EM (2007) Phytoremedation behaviour of some medicinal and aromatic plants to various pollutants. Journal of Field Crops Central Research Institute (Ankara) 16, 1–10.

Bagdonaitɹ E, Mártonfi P, Repčák M, Labokas J (2010) Variation in the contents of pseudohypericin and hypericin in Hypericum perforatum from Lithuania. Biochemical Systematics and Ecology 38, 634–640.
Variation in the contents of pseudohypericin and hypericin in Hypericum perforatum from Lithuania.Crossref | GoogleScholarGoogle Scholar |

Baranowska I, Srogi K, Wlochowicz A, Szczepanik K (2002) Determination of heavy metal contents in samples of medicinal herbs. Polish Journal of Environmental Studies 11, 467–471.

Council of Europe (2008) ‘European pharmacopoeia’. (EDQM Council of Europe: Strasbourg, France)

Crockett S, Robson N (2011) Taxonomy and chemotaxonomy of the genus Hypericum. Medicinal and Aromatic Plant Science and Biotechnology 5, 1–13.

Denholm J (2010) Complementary medicine and heavy metal toxicity in Australia. WebmedCentral TOXICOLOGY 1, WMC00535

Diaconu D, Diaconu R, Navrotescu T (2012) Estimation of the heavy metals in medicinal plants and their infusions. Ovidius University Annals of Chemistry 23, 115–120.

Element Concentration Cadastre in Ecosystems (1994) Element Concentration Cadasters in Ecosystems (ECCE) progress report, presented at the 25th General Assembly of International Union of Biological Sciences (IUBS: Paris, France)

Evstatieva L, Hardalova R, Stoyanova K (2007) Medicinal plants in Bulgaria: diversity, legislation, conservation and trade. Phytologia Balcanica 13, 415–427.

Gentscheva G, Stafilov T, Ivanova E (2010) Determination of some essential and toxic elements in herbs from Bulgaria and Macedonia using atomic spectrometry. Eurasian Journal of Analytical Chemistry 5, 104–111.

Kabata-Pendias A, Pendias H (1984) ‘Trace elements in soils and plants’. (CRC Press: Boca Raton, FL, USA)

Kazakou E, Dimitrakopoulos PG, Baker AJM, Reeves RD, Troumbis AY (2008) Hypotheses, mechanisms and trade-off s of tolerance and adaptation to serpentine soils: from species to ecosystem level. Biological Reviews of the Cambridge Philosophical Society 83, 495–508.

Kazakou E, Adamidis GC, Baker AJM, Reeves RD, Godino M, Dimitrakopoulos PG (2010) Species adaptation in serpentine soils in Lesbos Island (Greece): metal hyperaccumulation and tolerance. Plant and Soil 332, 369–385.
Species adaptation in serpentine soils in Lesbos Island (Greece): metal hyperaccumulation and tolerance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXntlGhur4%3D&md5=e483f8fb1cca5d9d90c2149744cd2e64CAS |

Khan SA, Khan L, Hussain I, Marwat KB, Akhtar N (2008) Profile of heavy metals in selected medicinal plants. Pakistan Journal of Weed Science and Research 14, 101–110.

Kosalec I, Cvek J, Tomi S (2009) Contaminants of medicinal herbs and herbal products. Archives of Industrial Hygiene and Toxicology 60, 485–501.
Contaminants of medicinal herbs and herbal products.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhslKhurc%3D&md5=c7a1f896d1dc9b5921aaab71e17c30fcCAS | 20061249PubMed |

Kostić D, Mitić S, Zarubica A, Mitić M, Veličković J, Randjelović S (2011) Content of trace metals in medicinal plants and their extracts. Hemijska industrija 65, 165–170.
Content of trace metals in medicinal plants and their extracts.Crossref | GoogleScholarGoogle Scholar |

Krasteva I, Nedelcheva A, Pavlova D, Zdraveva P, Nikolov S (2013) Influence of the serpentine on the content of flavonoids in Hypericum populations growing in Bulgaria. African Journal of Pharmacy and Pharmacognosy 7, 1762–1765.
Influence of the serpentine on the content of flavonoids in Hypericum populations growing in Bulgaria.Crossref | GoogleScholarGoogle Scholar |

Kruckeberg A (1984) ‘California serpentines: flora, vegetation, geology, soil and management problems’. (University of California Press: Berkeley, CA, USA)

Masarovičová E, Králová K (2012) Plant–heavy metal interaction: phytoremediation, biofortification and nanoparticles. In ‘Advances in selected plant physiology aspect’. (Eds G Montanaro, B Dichio) pp. 75–102. (InTech: Rijeka, Croatia)

Mihaljev Ž, Živkov-Balos M, Cupić Z, Jaksić S (2014) Levels of some microelements and essential heavy metals in herbal teas in Serbia. Acta Poloniae Pharmaceutica - Drug Research 71, 385–391.

Obratov-Petković D, Bjedov I, Belanović S (2008) The content of heavy metals in the leaves of Hypericum perforatum L. on serpentinite soils in Serbia. Glasnik Sumarskog fakulteta 98, 143–153.
The content of heavy metals in the leaves of Hypericum perforatum L. on serpentinite soils in Serbia.Crossref | GoogleScholarGoogle Scholar |

Owen JD (2013) Investigation of the elemental profiles of Hypericum perforatum as used in herbal remedies. PhD thesis, University of Hertfordshire, UK.

Pavlova D, Karadjova I (2012) Chemical analysis of Teucrium species (Lamiaceae) growing on serpentine soils in Bulgaria. Journal of Plant Nutrition and Soil Science 175, 891–899.
Chemical analysis of Teucrium species (Lamiaceae) growing on serpentine soils in Bulgaria.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xhsleks7bP&md5=07fa2bab3a458e5119da4e77c1308b42CAS |

Pavlova D, Karadjova I (2013) Toxic elements profiles in selected medicinal plants growing on serpentines in Bulgaria. Biological Trace Element Research 156, 288–297.
Toxic elements profiles in selected medicinal plants growing on serpentines in Bulgaria.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhs1yqsrvI&md5=ac1ed4743858413697c799b288011861CAS | 24170367PubMed |

Peško M, Králová K, Masarovičová E (2010) Response of Hypericum perforatum plants to supply of cadmium compounds containing different forms of selenium. Ecological Chemistry and 17, 279–287.

Radanović D, Antić-Mladenović S, Jakovljević M (2002) Influence of some soil characteristics on heavy metal content in Hypericum perforatum L. and Achillea millefolium L. Acta Horticulturae 576, 295–301.

Rahnavard A, Daneshian J, Heravan E, Valadavadi S, Golein B (2012) Investigation of the most important secondary metabolites of St John’s wort (Hypericum perforatum L.) in Caspian climate. Journal of Food Agriculture and Environment 10, 375–381.

Ranđelović SS, Kostić DA, Zarubica AR, Mitić SS, Mitić MN (2013) The correlation of metal content in medicinal plants and their water extracts. Hemijska industrija 67, 585–591.
The correlation of metal content in medicinal plants and their water extracts.Crossref | GoogleScholarGoogle Scholar |

Redzić S (2010) Wild medicinal plants and their usage in traditional human therapy (Southern Bosnia and Herzegovina, W. Balkan). Journal of Medicinal Plants Research 4, 1003–1027.

Reeves R (2006) Hyperaccumulation of trace elements by plants. In ‘Phytoremediation of metal-contaminated soils’. (Eds JL Morel, G Echevarria, N Goncharova) pp. 25–52 (Springer: Dordrecht, The Netherlands)

van der Ent A, Baker AJM, Reeves R, Pollard J, Schat H (2013) Hyperaccumulators of metal and metalloid trace elements: facts and fiction. Plant and Soil 362, 319–334.
Hyperaccumulators of metal and metalloid trace elements: facts and fiction.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhvV2ks7bF&md5=bee356700f51fe2ae7218429ac2a5674CAS |

Velchev V, Ganchev S, Bondev I (1982) Vegetation belts. In ‘Geography of Bulgaria. Physical Geography. Natural conditions and resources’. (Ed. Zh Galabov) pp. 439–440 (Bulgarian Academy of Sciences: Sofia, Bulgaria)

WHO (World Health Organization) (1998) ‘Quality control methods for medicinal plant materials.’ (WHO: Geneva, Switzerland)

WHO (World Health Organization) (2007) ‘Guidelines for assessing quality of herbal medicines with reference to contaminants and residue’. (WHO: Geneva, Switzerland) Available at http://apps.who.int/medicinedocs/index/assoc/s14878e/s14878e.pdf [Verified 1 September 2014]