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Contents Vol 69(4)

Cationic Micelles Based on Polyhedral Oligomeric Silsesquioxanes for Enhanced Gene Transfection

Connie K. Liu A , Qingqing Dou A D , Sing Shy Liow A , Jatin N. Kumar A and Xian Jun Loh A B C D
+ Author Affiliations
- Author Affiliations

A Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis, #08-03 Singapore 138634, Republic of Singapore.

B Department of Materials Science and Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576, Republic of Singapore.

C Singapore Eye Research Institute, 11 Third Hospital Avenue, Singapore 168751, Republic of Singapore.

D Corresponding authors. Email: douq@imre.a-star.edu.sg; lohxj@imre.a-star.edu.sg

Australian Journal of Chemistry 69(4) 363-371 https://doi.org/10.1071/CH15636
Submitted: 10 October 2015  Accepted: 2 November 2015   Published: 30 November 2015

Abstract

The synthesis and gene transfection efficiency of a series of amphiphilic copolymers, poly(2-(dimethylamino)ethyl methacrylate)-poly (methacrylate isobutyl polyhedral oligomeric silsesquioxane) (PDMAEMA-POSS) copolymers are reported. The hydrophobic POSS interior allows a cell-sensitizing drug such as paclitaxel to be incorporated, whereas the cationic and hydrophilic PDMAEMA corona allows the complexation of anionic DNA to form a nano-sized polyplex. These drug-encapsulated copolymers display excellent gene transfection efficiency compared with polyethylenimine or PDMAEMA homopolymers.


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