Characterisation of high- and low-molecular-weight glutenin subunit genes in Chinese winter wheat cultivars and advanced lines using allele-specific markers and SDS-PAGE

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doi:10.1071/CP09164

Characterisation of high- and low-molecular-weight glutenin subunit genes in Chinese winter wheat cultivars and advanced lines using allele-specific markers and SDS-PAGE

F. P. Yang ^A ^B ^G, L. H. Wang ^A ^G, J. W. Wang ^A ^C, X. Y. He ^A, X. K. Zhang ^C, X. W. Shang ^D, W. X. Yang ^B, X. C. Xia ^A ^F, Z. H. He ^A ^E ^F

^A Institute of Crop Science, National Wheat Improvement Centre/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing 100081, China.
^B Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China.
^C College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China.
^D College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
^E International Maize and Wheat Improvement Centre (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing 100081, China.
^F Corresponding authors. Emails: xiaxianchun@caas.net.cn; zhhe@public3.bta.net.cn
^G F. P. Yang and L. H. Wang contributed equally to this work.





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Crop and Pasture Science 61(1) 84–91 http://dx.doi.org/10.1071/CP09164
Submitted: 9 June 2009 Accepted: 16 September 2009 Published online: 17 December 2009

Abstract

Wheat end-use product quality is highly influenced by the composition and quantity of high- and low-molecular-weight glutenin subunits (HMW-GS and LMW-GS). In the present study, 224 Chinese wheat cultivars and advanced lines were characterised for the HMW-GS and LMW-GS with allele-specific PCR markers and sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that 56 cultivars (25.0%) carried the allele Glu-D1-1d (Dx5), while 80 cultivars (35.7%) with the allele Glu-B1-2a (By8) produced a 527-bp specific band. Fourteen genotypes (6.3%) with the allele Glu-B1e (Bx20) yielded a 701-bp amplicon with the marker Mar and a 753-bp specific PCR fragment with the marker ZSBy9aF1/R3. Glu-B1h (Bx14+By15) was present in only 1 genotype, and 2 cultivars contained the allele Glu-B1f (Bx13+By16) identified with the marker ZSBy9F2/R2. Four genotypes (1.8%) with the allele Glu-B1-1d (Bx6) gave 695-bp and 830-bp bands, and 5 genotypes (2.2%) with the allele Glu-B1i (Bx17+By18) amplified a 659-bp fragment using the marker Bx. One hundred and six cultivars (47.3%) had the allele Glu-B1-2b (By9), amplifying a 663-bp fragment with the marker ZSBy9aF1/R3; 34 genotypes (15.8%) contained the allele Glu-B3d, generating a 662-bp PCR fragment with the marker gluB3d. Fifteen cultivars (7.0%) with the allele Glu-B3b yielded 1570-bp and 750-bp PCR amplicons with the markers gluB3b and gluB3bef, respectively. The allele Glu-B3h was found in 7 cultivars, generating a 1022-bp PCR fragment with the marker gluB3h. The genotypes detected by SDS-PAGE were mostly consistent with those identified by molecular markers, confirming the utility of the molecular markers. The information for the HMW-GS and LMW-GS in Chinese wheat cultivars will be useful in wheat breeding programs.

Keywords: Triticum aestivum L., HMW-GS, LMW-GS, SDS-PAGE.