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RESEARCH ARTICLE

Effects of lucerne genotype on morphology, biomass production and nitrogen content of lucerne and tall fescue in mixed pastures

Amel Maamouri A , Gaëtan Louarn A , François Gastal A , Vincent Béguier B and Bernadette Julier A C
+ Author Affiliations
- Author Affiliations

A INRA, UR4 URP3F, CS 80006, F-86600 Lusignan, France.

B Jouffray-Drillaud, La Litière, F-86600 Saint-Sauvant, France.

C Corresponding author. Email: bernadette.julier@lusignan.inra.fr

Crop and Pasture Science 66(2) 192-204 https://doi.org/10.1071/CP14164
Submitted: 19 June 2014  Accepted: 15 September 2014   Published: 27 January 2015

Abstract

Cultivation of legume–grass mixtures ensures a high, protein-rich forage yield with no nitrogen (N) fertilisation. The maintenance of a reasonable proportion of each species over time may depend on the variety. In mixtures of lucerne (Medicago sativa L.) and grass, the effect of genetic variation on biomass and N accumulation is little described. We analysed how lucerne genetic variation affects agronomic traits. The experiment included 46 lucerne and two tall fescue (Festuca arundinacea Schreb.) genotypes tested in microplots of three lucerne and four fescue clones, evaluating biomass production, plant height, stem number and N content in eight cuts in 2011 and 2012. There was a wide genetic variation among lucerne genotypes but no significant statistical interaction between lucerne and fescue genotypes. This suggests that agronomic value of lucerne genotypes for mixtures can be evaluated with any grass genotype. On average, the grass plants grown with highly productive lucerne genotypes had an increased leaf elongation and N status but a reduced tiller number, which could limit their persistence. This is the first observation that the choice of lucerne genotype determines morphology, biomass production and N absorption of both lucerne and tall fescue grown in mixture.

Additional keywords: competition, forage, grassland, mixture.


References

Afkar S, Karimzadeh G, Jafari A (2010) A study of morphological variation in some genotypes of Festuca arundinacea using multivariate analysis. Iranian Journal of Field Crop Science 40, 151–160.

Annicchiarico P (2003) Breeding white clover for increased ability to compete with associated grasses. The Journal of Agricultural Science 140, 255–266.
Breeding white clover for increased ability to compete with associated grasses.Crossref | GoogleScholarGoogle Scholar |

Annicchiarico P (2006) Prediction of indirect selection for seed and forage yield of lucerne based on evaluation under spaced planting. Plant Breeding 125, 641–643.
Prediction of indirect selection for seed and forage yield of lucerne based on evaluation under spaced planting.Crossref | GoogleScholarGoogle Scholar |

Annicchiarico P, Piano E (1994) Interference effects in white clover genotypes grown as pure stands and binary-mixtures with different grass species and varieties. Theoretical and Applied Genetics 88, 153–158.
Interference effects in white clover genotypes grown as pure stands and binary-mixtures with different grass species and varieties.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC2c7isVOqtg%3D%3D&md5=e73b68b5136b1d6153df1d3e18c7ff94CAS | 24185920PubMed |

Annicchiarico P, Proietti S (2010) White clover selected for enhanced competitive ability widens the compatibility with grasses and favours the optimization of legume content and forage yield in mown clover–grass mixtures. Grass and Forage Science 65, 318–324.
White clover selected for enhanced competitive ability widens the compatibility with grasses and favours the optimization of legume content and forage yield in mown clover–grass mixtures.Crossref | GoogleScholarGoogle Scholar |

Bahmani I, Hazard L, Varlet-Grancher C, Betin M, Lemaire G, Matthew C, Thom E (2000) Differences in tillering of long- and short-leaved perennial ryegrass genetic lines under full light and shade treatments. Crop Science 40, 1095–1102.
Differences in tillering of long- and short-leaved perennial ryegrass genetic lines under full light and shade treatments.Crossref | GoogleScholarGoogle Scholar |

Barnes D, Heichel G, Vance C, Peaden R (1990) Registration of ‘Ineffective Agate’ and ‘Ineffective Saranac’ non-N2-fixing alfalfa germplasms. Crop Science 30, 752–753.

Burity H, Ta T, Faris M, Coulman B (1989) Estimation of nitrogen fixation and transfer from alfalfa to associated grasses in mixed swards under field conditions. Plant and Soil 114, 249–255.
Estimation of nitrogen fixation and transfer from alfalfa to associated grasses in mixed swards under field conditions.Crossref | GoogleScholarGoogle Scholar |

Chambers JM (1992) Linear models. In ‘Statistical models in S’. (Eds JM Chambers, TJ Hastie) pp. 95–144. (Chapman & Hall/CRC: Boca Raton, FL, USA)

Chamblee DS, Colins M (1988) Relationships with other species in a mixture. In ‘Alfalfa and alfalfa improvement’. (Eds AA Hanson, DK Barnes, RR Hill) pp. 439–461. (ASA: Madison, WI)

Corre-Hellou G, Fustec J, Crozat Y (2006) Interspecific competition for soil N and its interaction with N-2 fixation, leaf expansion and crop growth in pea-barley intercrops. Plant and Soil 282, 195–208.
Interspecific competition for soil N and its interaction with N-2 fixation, leaf expansion and crop growth in pea-barley intercrops.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XmtVOqurg%3D&md5=8cdff252cf089ebdb1ba8fe4983c50fbCAS |

Crochemore ML, Huyghe C, Kerlan MC, Durand F, Julier B (1996) Partitioning and distribution of RAPD variation in a set of populations of the Medicago sativa complex. Agronomie 16, 421–432.
Partitioning and distribution of RAPD variation in a set of populations of the Medicago sativa complex.Crossref | GoogleScholarGoogle Scholar |

Cruz PA, Sinoquet H (1994) Competition for light and nitrogen during a regrowth cycle in a tropical forage mixture. Field Crops Research 36, 21–30.
Competition for light and nitrogen during a regrowth cycle in a tropical forage mixture.Crossref | GoogleScholarGoogle Scholar |

Dodd M, McGowan A, Power I, Thorrold B (2005) Effects of variation in shade level, shade duration and light quality on perennial pastures. New Zealand Journal of Agricultural Research 48, 531–543.
Effects of variation in shade level, shade duration and light quality on perennial pastures.Crossref | GoogleScholarGoogle Scholar |

Flajoulot S, Ronfort J, Baudouin P, Barre P, Huguet T, Huyghe C, Julier B (2005) Genetic diversity among alfalfa (Medicago sativa) cultivars coming from a single breeding program, using SSR markers. Theoretical and Applied Genetics 111, 1420–1429.
Genetic diversity among alfalfa (Medicago sativa) cultivars coming from a single breeding program, using SSR markers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXht1aitL%2FM&md5=4c21dd72911167b62178b806daa9af3eCAS | 16151797PubMed |

Fustec J, Lesuffleur F, Mahieu S, Cliquet JB (2010) Nitrogen rhizodeposition of legumes. A review. Agronomy for Sustainable Development 30, 57–66.
Nitrogen rhizodeposition of legumes. A review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXjs12ks7w%3D&md5=fd704290fda02fd08d202de693826ec6CAS |

Gaborcik N (1994) Variability of morphological and physiological traits in tall fescue (Festuca arundinacea Schreb.). Rocenka - Geneticke zdroje rastlin 38–42.

Gallais A (1970) Modèle pour l’analyse des relations d’associations binaires. Biométrie-Praximétrie 11, 51–80.

Garg N, Geetanjali (2007) Symbiotic nitrogen fixation in legume nodules: process and signaling. A review. Agronomy for Sustainable Development 27, 59–68.
Symbiotic nitrogen fixation in legume nodules: process and signaling. A review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXlt1Grtr8%3D&md5=d832acafa7dc37a849154ad74acfca63CAS |

Gastal F, Lemaire G (2002) N uptake and distribution in crops: an agronomical and ecophysiological perspective. Journal of Experimental Botany 53, 789–799.
N uptake and distribution in crops: an agronomical and ecophysiological perspective.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XivFSntL0%3D&md5=0437d8fe5c67cd087f188628a27de3e7CAS | 11912222PubMed |

Gastal F, Belanger G, Lemaire G (1992) A model of the leaf extension rate of tall fescue in response to nitrogen and temperature. Annals of Botany 70, 437–442.

Gastal F, Farruggia A, Hacquet J (2001) The nitrogen nutrition index of grass swards can be evaluated through determination of N concentration of upper leaves. In ‘11th Nitrogen Workshop’. UR 0004 Station d’écophysiologie des fourrages: secteur bioclimatologie, Centre de recherche de Poitou-Charentes, 86600 Lusignan, France. pp. 449–450. (INRA Editions: Paris)

Gastal F, Lemaire G, Durand JL, Louarn G (2014) Quantifying crop responses to nitrogen and avenues to improve nitrogen-use efficiency. In ‘Crop physiology—Application for genetic improvement and agronomy’. (Eds VO Sadras, D Calderini) (Academic Press/Elsevier: Amsterdam)

Gautier H, Varlet-Grancher C, Hazard L (1999) Tillering responses to the light environment and to defoliation in populations of perennial ryegrass (Lolium perenne L.) selected for contrasting leaf length. Annals of Botany 83, 423–429.
Tillering responses to the light environment and to defoliation in populations of perennial ryegrass (Lolium perenne L.) selected for contrasting leaf length.Crossref | GoogleScholarGoogle Scholar |

Guy P (1989) Essais multilocaux d’associations trèfle violet-graminées. Fourrages 117, 29–48.

Hardarson G, Danso S, Zapata F (1988) Dinitrogen fixation measurements in alfalfa-ryegrass swards using nitrogen-15 and influence of the reference crop. Crop Science 28, 101–105.
Dinitrogen fixation measurements in alfalfa-ryegrass swards using nitrogen-15 and influence of the reference crop.Crossref | GoogleScholarGoogle Scholar |

Haynes R (1980) Competitive aspects of the grass–legume association. Advances in Agronomy 33, 227–261.
Competitive aspects of the grass–legume association.Crossref | GoogleScholarGoogle Scholar |

Høgh-Jensen H, Schjoerring J (1994) Measurement of biological dinitrogen fixation in grassland: comparison of the enriched 15N dilution and the natural 15N abundance methods at different nitrogen application rates and defoliation frequencies. Plant and Soil 166, 153–163.
Measurement of biological dinitrogen fixation in grassland: comparison of the enriched 15N dilution and the natural 15N abundance methods at different nitrogen application rates and defoliation frequencies.Crossref | GoogleScholarGoogle Scholar |

Høgh-Jensen H, Loges R, Jørgensen FV, Winther FP, Jensen ES (2004) An empirical model for quantification of symbiotic nitrogen fixation in grass–clover mixtures. Agricultural Systems 82, 181–194.
An empirical model for quantification of symbiotic nitrogen fixation in grass–clover mixtures.Crossref | GoogleScholarGoogle Scholar |

Hooper DU, Chapin FS, Ewel JJ, Hector A, Inchausti P, Lavorel S, Lawton JH, Lodge DM, Loreau M, Naeem S, Schmid B, Setala H, Symstad AJ, Vandermeer J, Wardle DA (2005) Effects of biodiversity on ecosystem functioning: A consensus of current knowledge. Ecological Monographs 75, 3–35.
Effects of biodiversity on ecosystem functioning: A consensus of current knowledge.Crossref | GoogleScholarGoogle Scholar |

Huyghe C, De Vliegher A, Van Gils B, Peeters A (2014) ‘Grassland and herbivore production in Europe and effects of common policies.’ (Editions Quae: Versailles, France) www.quae.com/fr/r3371-grasslands-and-herbivore-production-in-europe-and-effects-of-common-policies.html

Julier B, Huyghe C (1997) Effect of growth and cultivar on alfalfa digestibility in a multi-site trial. Agronomie 17, 481–489.
Effect of growth and cultivar on alfalfa digestibility in a multi-site trial.Crossref | GoogleScholarGoogle Scholar |

Julier B, Huyghe C, Ecalle C (2000) Within- and among-cultivar genetic variation in alfalfa: forage quality, morphology, and yield. Crop Science 40, 365–369.
Within- and among-cultivar genetic variation in alfalfa: forage quality, morphology, and yield.Crossref | GoogleScholarGoogle Scholar |

Knott EA, Mundt CC (1990) Mixing ability analysis of wheat cultivar mixtures under diseased and nondiseased conditions. Theoretical and Applied Genetics 80, 313–320.
Mixing ability analysis of wheat cultivar mixtures under diseased and nondiseased conditions.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC2c7lvVemtA%3D%3D&md5=b653c2b0cc620cd4bace339b6e8dbcc5CAS | 24220962PubMed |

Lemaire G, Allirand JM (1993) Relation entre croissance et qualité de la luzerne: interaction génotype-mode d’exploitation. Fourrages 134, 183–198.

Lemaire G, Cruz P, Gosse G, Chartier M (1985) Etude des relations entre la dynamique de prélèvement d’azote et la dynamique de croissance en matière sèche d’un peuplement de luzerne (Medicago sativa L.). Agronomie 5, 685–692.
Etude des relations entre la dynamique de prélèvement d’azote et la dynamique de croissance en matière sèche d’un peuplement de luzerne (Medicago sativa L.).Crossref | GoogleScholarGoogle Scholar |

Lemaire G, Onillon B, Gosse G, Chartier M, Allirand JM (1991) Nitrogen distribution within a lucerne canopy during regrowth: relation with light distribution. Annals of Botany 68, 483–488.

Lemaire G, Khaity M, Onillon B, Allirand JM, Chartier M, Gosse G (1992) Dynamics of accumulation and partitioning of N in leaves, stems and roots of lucerne (Medicago sativa L.) in a dense canopy. Annals of Botany 70, 429–435.

Lemaire G, Wilkins R, Hodgson J (2005) Challenges for grassland science: managing research priorities. Agriculture, Ecosystems & Environment 108, 99–108.
Challenges for grassland science: managing research priorities.Crossref | GoogleScholarGoogle Scholar |

Louarn G, Corre-Hellou G, Fustec J, Lô-Pelzer E, Julier B, Litrico I, Hinsinger P, Lecomte C (2010) Déterminants écologiques et physiologiques de la productivité et de la stabilité des associations graminées-légumineuses. Innovations Agronomiques 11, 79–99.

Louarn G, Silva D, Godin C, Combes D (2012) Simple envelope-based reconstruction methods can infer light partitioning among individual plants in sparse and dense herbaceous canopies. Agricultural and Forest Meteorology 166–167, 98–112.
Simple envelope-based reconstruction methods can infer light partitioning among individual plants in sparse and dense herbaceous canopies.Crossref | GoogleScholarGoogle Scholar |

Malézieux E, Crozat Y, Dupraz C, Laurans M, Makowski D, Ozier-Lafontaine H, Rapidel B, de Tourdonnet S, Valantin-Morison M (2009) Mixing plant species in cropping systems: concepts, tools and models. A review. Agronomy for Sustainable Development 29, 43–62.
Mixing plant species in cropping systems: concepts, tools and models. A review.Crossref | GoogleScholarGoogle Scholar |

Martiniello P (1998) Influence of agronomic factors on the relationship between forage production and seed yield in perennial forage grasses and legumes in a Mediterranean environment. Agronomie 18, 591–601.
Influence of agronomic factors on the relationship between forage production and seed yield in perennial forage grasses and legumes in a Mediterranean environment.Crossref | GoogleScholarGoogle Scholar |

Martiniello P, Santis G, Iannucci A (1997) Mating effects on phenotypic variability for agronomic traits in populations of tall fescue (Festuca arundinacea Schreb.). Journal of Genetics & Breeding 51, 7–15.

Melton B, Moutray J, Bouton J (1988) Geographic adaptation and cultivar selection. In ‘Alfalfa and alfalfa improvement’. (Eds AA Hanson, DK Barnes, RR Hill) pp. 595–620. (ASA, CSSA, SSSA: Madison, WI, USA)

Mosimann E, Jeangros B, Suter D, Briner HU (2007) Alfalfa and bromegras variety trials (2004–2006). Revue Suisse D’Agriculture 39, 189–192.

Nyfeler D, Huguenin-Elie O, Suter M, Frossard E, Lüscher A (2011) Grass–legume mixtures can yield more nitrogen than legume pure stands due to mutual stimulation of nitrogen uptake from symbiotic and non-symbiotic sources. Agriculture, Ecosystems & Environment 140, 155–163.
Grass–legume mixtures can yield more nitrogen than legume pure stands due to mutual stimulation of nitrogen uptake from symbiotic and non-symbiotic sources.Crossref | GoogleScholarGoogle Scholar |

Rotili P (1985) Composition of two-component pasture mixtures. Discussion of trial results obtained at Lodi. Rivista di Agronomia 19, 170–177.

Sackville-Hamilton NR (2001) Measurement of competition and competition effects in pastures. In ‘Competition and succession in pastures’. (Eds PG Tow, A Lazenby) pp. 15–42. (CAB International: Wallingford, UK)

Schwinning S, Weiner J (1998) Mechanisms determining the degree of size asymmetry in competition among plants. Oecologia 113, 447–455.
Mechanisms determining the degree of size asymmetry in competition among plants.Crossref | GoogleScholarGoogle Scholar |

Ta T, Faris M (1987) Effects of alfalfa proportions and clipping frequencies on timothy-alfalfa mixtures. II. Nitrogen fixation and transfer. Agronomy Journal 79, 820–824.
Effects of alfalfa proportions and clipping frequencies on timothy-alfalfa mixtures. II. Nitrogen fixation and transfer.Crossref | GoogleScholarGoogle Scholar |

Tucak M, Popovic S, Cupic T, Simic G, Gantner R, Meglic V (2009) Evaluation of alfalfa germplasm collection by multivariate analysis based on phenotypic traits. Romanian Agricultural Research 26, 47–52.

Varlet-Grancher C, Gautier H, Bonhomme R (2000) Réponses morphogénétiques des plantes aux modifications de qualité de la lumière intervenant au sein du peuplement végétal. In ‘Fonctionnement des peuplements végétaux sous contraintes environnementales’. (Eds P Maillard, R Bonhomme) pp. 353–383. (Quae: Paris)

Volenec J (1985) Leaf area expansion and shoot elongation of diverse alfalfa germplasms. Crop Science 25, 822–827.
Leaf area expansion and shoot elongation of diverse alfalfa germplasms.Crossref | GoogleScholarGoogle Scholar |

Volenec J, Cherney J, Johnson K (1987) Yield components, plant morphology, and forage quality of alfalfa as influenced by plant population. Crop Science 27, 321–326.
Yield components, plant morphology, and forage quality of alfalfa as influenced by plant population.Crossref | GoogleScholarGoogle Scholar |

Wilkinson GN, Rogers CE (1973) Symbolic descriptions of factorial models for analysis of variance. Applied Statistics 22, 392–399.
Symbolic descriptions of factorial models for analysis of variance.Crossref | GoogleScholarGoogle Scholar |

Williams TA, Evans DR, Rhodes I, Abberton MT (2003) Long-term performance of white clover varieties grown with perennial ryegrass under rotational grazing by sheep with different nitrogen applications. The Journal of Agricultural Science 140, 151–159.
Long-term performance of white clover varieties grown with perennial ryegrass under rotational grazing by sheep with different nitrogen applications.Crossref | GoogleScholarGoogle Scholar |

Wilman D, Pearse P (1984) Effects of applied nitrogen on grass yield, nitrogen content, tillers and leaves in field swards. Journal of Agricultural Science, UK 103, 201–211.
Effects of applied nitrogen on grass yield, nitrogen content, tillers and leaves in field swards.Crossref | GoogleScholarGoogle Scholar |

Zannone L, Assemat L, Rotili P, Jacquard P (1983) An experimental study of intraspecific competition within several forage crops. Agronomie 3, 451–459.
An experimental study of intraspecific competition within several forage crops.Crossref | GoogleScholarGoogle Scholar |

Zannone L, Rotili P, Paoletti R, Scotti C (1986) Experimental studies of grass-legume associations. Agronomie 6, 931–940.
Experimental studies of grass-legume associations.Crossref | GoogleScholarGoogle Scholar |