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Selenium fortification of hydroponically grown corn salad (Valerianella locusta)

Nicola Tomasi A , Roberto Pinton A C , Stefano Gottardi A , Tanja Mimmo B , Matteo Scampicchio B and Stefano Cesco B
+ Author Affiliations
- Author Affiliations

A Dipartimento di Scienze Agrarie e Ambientali, University of Udine, 33100 Udine, Italy.

B Faculty of Science and Technology, Free University of Bolzano, 39100 Bolzano, Italy.

C Corresponding author. Email: roberto.pinton@uniud.it

Crop and Pasture Science 66(11) 1128-1136 https://doi.org/10.1071/CP14218
Submitted: 1 August 2014  Accepted: 12 November 2014   Published: 14 September 2015

Abstract

There is increasing interest in the hydroponic technology to produce leafy vegetables for ready-to-eat salads. Optimisation of the growing system can lead to higher yield and/or improved nutritional value of the product. Selenium (Se) is an essential element for animal and humans, with quite a narrow range between deficiency and toxicity, whereas it is assumed beneficial for plants. In the present study, two cultivars (Gala and Baron) of corn salad (Valerianella locusta (L.) Laterr.) were used to test the possibility to increase Se content in the edible parts (leaves). Effects on yield, nitrate content, and accumulation of sulfur (S) and S-containing amino acids and Se and Se-containing amino acids were studied. Results showed that corn salad tolerates selenate (Na2SeO4) concentrations ranging from 10 to 40 µm in the nutrient solution, with plants accumulating Se at levels compatible with the need in human diets at 10 µm selenate. Se-treated plants showed some benefits with respect to a decrease of nitrate concentration and increase of pigment contents (chlorophylls and carotenoids). At 10 µm selenate, Se-cysteine and Se-methionine were produced, without affecting non-protein thiols or cysteine and methionine contents. At the higher Se supply, sulfate accumulated in the leaves with a parallel decrease in the amount of S-amino acids and a rise in the relative amount of Se-amino acids. Based on the chemical analyses, cv. Gala showed better tolerance than cv. Baron to moderate selenate supply (40 µm).

Additional keywords: biofortification, floating system, horticultural crops, sulfur assimilation, Se-cysteine, Se-methionine.


References

Abd-Elmoniem EM, Abdrabbo MA, Farag AA, Medany MA (2006) Hydroponics for food production: comparison of open and closed systems on yield and consumption of water and nutrient. In ‘Proceedings 2nd International Conference on Water Resources and Arid Environments’. Riyadh. p. 28. (ICWRAE: Riyadh, Saudi Arabia)

Bienfait HF, van den Briel W, Mesland-Mul NT (1985) Free space iron pools in roots: generation and mobilization. Plant Physiology 78, 596–600.
Free space iron pools in roots: generation and mobilization.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2MXlsF2ks78%3D&md5=84337256fc9069411801000f7f0bf361CAS | 16664289PubMed |

Bondioli P, Rivolta G (2013) Insect oils: the composition of oil extracted from Mosca carnaria (Sarcophaga carnaria L.) larva. Rivista Italiana delle sostanze grasse 90.1, 5–8.

Cataldo DA, Maroon M, Schrader LE, Youngs VL (1975) Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Communications in Soil Science and Plant Analysis 6, 71–80.
Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2MXhs1Kqt7Y%3D&md5=c03609f6897864ae2a043b0fd3301ab5CAS |

Cesco S, Romheld V, Varanini Z, Pinton R (2000) Solubilization of iron by water-extractable humic substances. Journal of Plant Nutrition and Soil Science 163, 285–290.
Solubilization of iron by water-extractable humic substances.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXks1Wlsb4%3D&md5=49eb630d4d94c6e2e220918c64a42866CAS |

Comar CL, Zscheile FP (1942) Analysis of plant extracts for chlorophylls a and b by a photoelectric spectrophotometric method. Plant Physiology 17, 198–209.
Analysis of plant extracts for chlorophylls a and b by a photoelectric spectrophotometric method.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaH3sXjslaqtQ%3D%3D&md5=d87a8639287c69e5cdd5a0d23722bee1CAS | 16653768PubMed |

Cortella G, Saro O, De Angelis A, Ceccotti L, Tomasi N, Dalla Costa L, Manzocco L, Pinton R, Mimmo T, Cesco S (2014) Temperature control of nutrient solution in floating system cultivation. Applied Thermal Engineering 73, 1055–1065.
Temperature control of nutrient solution in floating system cultivation.Crossref | GoogleScholarGoogle Scholar |

Dalla Costa L, Tomasi N, Gottardi S, Iacuzzo F, Cortella G, Manzocco L, Pinton R, Mimmo T, Cesco S (2011) The effect of growth medium temperature on corn salad [Valerianella locusta (L.) Laterr] baby leaf yield and quality. HortScience 46, 1619–1625.

Diwadkar-Navsariwala V, Prins GS, Swanson SM, Birch LA, Ray VH, Hedayat S, Lantvit DL, Diamond AM (2006) Selenoprotein deficiency accelerates prostate carcinogenesis in a transgenic model. Proceedings of the National Academy of Sciences of the United States of America 103, 8179–8184.
Selenoprotein deficiency accelerates prostate carcinogenesis in a transgenic model.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xlt1ChtLo%3D&md5=18f7aa7eef2e73d8639cd35222616156CAS | 16690748PubMed |

Ellis DR, Salt DE (2003) Plants, selenium and human health. Current Opinion in Plant Biology 6, 273–279.
Plants, selenium and human health.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXjs1Git74%3D&md5=4b8fe54c9a4304518efcd042042ba6caCAS | 12753978PubMed |

Feist LJ, Parker DR (2001) Ecotypic variation in selenium accumulation among populations of Stanleya pinnata. New Phytologist 149, 61–69.
Ecotypic variation in selenium accumulation among populations of Stanleya pinnata.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXotVSjsg%3D%3D&md5=61aef34c401b9323b719dc6a672b1729CAS |

Feng R, Wei C, Tu S (2013) The roles of selenium in protecting plants against abiotic stresses. Environmental and Experimental Botany 87, 58–68.
The roles of selenium in protecting plants against abiotic stresses.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXit1KmsLc%3D&md5=ab248ad464f744c2b5ccc33626fe90deCAS |

Gottardi S, Iacuzzo F, Tomasi N, Cortella G, Manzocco L, Pinton R, Roemheld V, Mimmo T, Scampicchio M, Dalla Costa L, Cesco S (2012) Beneficial effects of silicon on hydroponically grown corn salad (Valerianella locusta (L.) Laterr) plants. Plant Physiology and Biochemistry 56, 14–23.
Beneficial effects of silicon on hydroponically grown corn salad (Valerianella locusta (L.) Laterr) plants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XotVakur8%3D&md5=70ac27550e27aac8a0b6ba723d95efadCAS | 22579940PubMed |

Hammel C, Kyriakopoulos A, Rosick U, Behne D (1997) Identification of selenocysteine and selenomethionine in protein hydrolysates by high-performance liquid chromatography of their o-phthaldialdehyde derivatives. Analyst 122, 1359–1364.
Identification of selenocysteine and selenomethionine in protein hydrolysates by high-performance liquid chromatography of their o-phthaldialdehyde derivatives.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXht1OlsLg%3D&md5=dd12fea8a59f92dc7596d426724c5e8bCAS | 9474814PubMed |

Hartikainen H, Xue T, Piironen V (2000) Selenium as an anti-oxidant and pro-oxidant in ryegrass. Plant and Soil 225, 193–200.
Selenium as an anti-oxidant and pro-oxidant in ryegrass.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXotVGiug%3D%3D&md5=fcab63bd3f1eb1f2e072bfd25e5ecb0fCAS |

Hsu F-C, Wirtz M, Heppel SC, Bogs J, Kraemer U, Khan MS, Bub A, Hell R, Rausch T (2011) Generation of Se-fortified broccoli as functional food: impact of Se fertilization on S metabolism. Plant, Cell & Environment 34, 192–207.
Generation of Se-fortified broccoli as functional food: impact of Se fertilization on S metabolism.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXisVGjsLs%3D&md5=595cd16f960913da32b06abdfac0f47bCAS |

Iacuzzo F, Gottardi S, Tomasi N, Savoia E, Tommasi R, Cortella G, Terzano R, Pinton R, Dalla Costa L, Cesco S (2011) Corn salad (Valerianella locusta (L.) Laterr.) growth in a water-saving floating system as affected by iron and sulfate availability. Journal of the Science of Food and Agriculture 91, 344–354.
Corn salad (Valerianella locusta (L.) Laterr.) growth in a water-saving floating system as affected by iron and sulfate availability.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsFKqtbbP&md5=3320f5d9c354f877abf84a608a68b75bCAS | 20960459PubMed |

Kumar M, Bijo AJ, Baghel RS, Reddy CRK, Jha B (2012) Selenium and Spermine alleviates cadmium induced toxicity in the red seaweed Gracilaria dura regulating antioxidant system and DNA methylation. Plant Physiology and Biochemistry 51, 129–138.
Selenium and Spermine alleviates cadmium induced toxicity in the red seaweed Gracilaria dura regulating antioxidant system and DNA methylation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhs1SgsrfN&md5=217d89aa98b7d25040c5b824d7030b94CAS | 22153249PubMed |

Li Z, Zhao X, Sandhu AK, Gu L (2010) Effects of exogenous abscisic acid on yield, antioxidant capacities, and phytochemical contents of greenhouse grown lettuces. Journal of Agricultural and Food Chemistry 58, 6503–6509.
Effects of exogenous abscisic acid on yield, antioxidant capacities, and phytochemical contents of greenhouse grown lettuces.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXltFykt7w%3D&md5=08ba6e4f25441684818d6462a0d7b6c1CAS | 20420437PubMed |

Lyons GH, Genc Y, Soole K, Stangoulis JCR, Liu F, Graham RD (2009) Selenium increases seed production in Brassica. Plant and Soil 318, 73–80.
Selenium increases seed production in Brassica.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXltVWrsb8%3D&md5=d3b79a332de03a9e15eb25b254993d79CAS |

Manzocco L, Foschia M, Tomasi N, Maifreni M, Dalla Costa L, Marino M, Cortella G, Cesco S (2011) Influence of hydroponic and soil cultivation on quality and shelf life of ready-to-eat lamb’s lettuce (Valerianella locusta L. Laterr). Journal of the Science of Food and Agriculture 91, 1373–1380.
Influence of hydroponic and soil cultivation on quality and shelf life of ready-to-eat lamb’s lettuce (Valerianella locusta L. Laterr).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXlsVOhtrY%3D&md5=a12d3dd140ab89a62034f1535d882761CAS | 21337577PubMed |

Marschner H (1995) ‘Mineral nutrition of higher plants.’ (Academic Press: London)

Navarro-Alarcón M, Lopez-Martinez MC (2000) Essentiality of selenium in the human body: relationship with different diseases. The Science of the Total Environment 249, 347–371.
Essentiality of selenium in the human body: relationship with different diseases.Crossref | GoogleScholarGoogle Scholar | 10813463PubMed |

Nikolic M, Cesco S, Monte R, Tomasi N, Gottardi S, Zamboni A, Pinton R, Varanini Z (2012) Nitrate transport in cucumber leaves is an inducible process involving an increase in plasma membrane H+-ATPase activity and abundance. BMC Plant Biology 12, 66
Nitrate transport in cucumber leaves is an inducible process involving an increase in plasma membrane H+-ATPase activity and abundance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhtFCgu7bE&md5=62fa96da12f478190e5ea8c4162dc67cCAS | 22571503PubMed |

Pilon-Smits EA, Quinn CF (2010) Selenium metabolism in plants. In ‘Cell biology of metals and nutrients’. (Eds R Hell, RR Mendel) pp. 225–241. (Springer: Berlin)

Pinton R, Cesco S, Iacolettig G, Astolfi S, Varanini Z (1999) Modulation of NO3 – uptake by water-extractable humic substances: involvement of root plasma membrane H+ATPase. Plant and Soil 215, 155–161.
Modulation of NO3 uptake by water-extractable humic substances: involvement of root plasma membrane H+ATPase.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXhslWqtLs%3D&md5=bd5ea34ffde0c060dd63924ad51464dbCAS |

Ramos SJ, Rutzke MA, Hayes RJ, Faquin V, Guiherme LRG, Li L (2011) Selenium accumulation in lettuce germplasm. Planta 233, 649–660.
Selenium accumulation in lettuce germplasm.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXjvVWgtLw%3D&md5=5a750ade3c6679dcf58d78f891a336ddCAS | 21153555PubMed |

Rayman MP (2002) The argument for increasing selenium intake. The Proceedings of the Nutrition Society 61, 203–215.
The argument for increasing selenium intake.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XltlOru70%3D&md5=f41bef07ab08495d57c49fd02eb41cfcCAS | 12133202PubMed |

Rayman MP (2008) Food-chain selenium and human health: emphasis on intake. British Journal of Nutrition 100, 254–268.

Rayman MP (2012) Selenium and human health. Lancet 379, 1256–1268.
Selenium and human health.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xjt1Orsb8%3D&md5=04c01a254eb87fb69f28a8e5f94b55b3CAS | 22381456PubMed |

Reeves DW, Mask PL, Wood CW, Delaney DP (1993) Determination of wheat nitrogen status with a hand-held chlorophyll meter: Influence of management practices. Journal of Plant Nutrition 16, 781–796.
Determination of wheat nitrogen status with a hand-held chlorophyll meter: Influence of management practices.Crossref | GoogleScholarGoogle Scholar |

Ríos JJ, Rosales MA, Blasco B, Cervilla LM, Romero L, Ruiz JM (2008) Biofortification of Se and induction of the antioxidant capacity in lettuce plants. Scientia Horticulturae 116, 248–255.
Biofortification of Se and induction of the antioxidant capacity in lettuce plants.Crossref | GoogleScholarGoogle Scholar |

Ríos JJ, Blasco B, Cervilla LM, Rubio-Wilhelmi MM, Rosales MA, Sanchez-Rodriguez E, Romero L, Ruiz JM (2010a) Nitrogen use efficiency in relation to different forms and application rates of Se in lettuce plants. Journal of Plant Growth Regulation 29, 164–170.
Nitrogen use efficiency in relation to different forms and application rates of Se in lettuce plants.Crossref | GoogleScholarGoogle Scholar |

Ríos JJ, Blasco B, Rosales MA, Sanchez-Rodriguez E, Leyva R, Cervilla LM, Romero L, Ruiz JM (2010b) Response of nitrogen metabolism in lettuce plants subjected to different doses and forms of selenium. Journal of the Science of Food and Agriculture 90, 1914–1919.

Rizzardo C, Tomasi N, Monte R, Varanini Z, Nocito FF, Cesco S, Pinton R (2012) Cadmium inhibits the induction of high-affinity nitrate uptake in maize (Zea mays L.) roots. Planta 236, 1701–1712.
Cadmium inhibits the induction of high-affinity nitrate uptake in maize (Zea mays L.) roots.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xhs12gsLvO&md5=64b2811c4beee83de637af02a4a9a7feCAS | 22983671PubMed |

Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG (1973) Selenium: biochemical role as a component of glutathione peroxidase. Science 179, 588–590.
Selenium: biochemical role as a component of glutathione peroxidase.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE3sXps1ensg%3D%3D&md5=79fcd3b398d7cf5781f052df5a98bcd9CAS | 4686466PubMed |

Santamaria P (2006) Nitrate in vegetables: toxicity, content, intake and EC regulation. Journal of the Science of Food and Agriculture 86, 10–17.
Nitrate in vegetables: toxicity, content, intake and EC regulation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtlektbfP&md5=6b8658338518bffdb2afc67b8ee8c89fCAS |

Santamaria P, Elia A, Serio F (2002) Effect of solution nitrogen concentration on yield, leaf element content, and water and nitrogen use efficiency of three hydroponically-grown rocket salad genotypes. Journal of Plant Nutrition 25, 245–258.
Effect of solution nitrogen concentration on yield, leaf element content, and water and nitrogen use efficiency of three hydroponically-grown rocket salad genotypes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XhtlKmsbg%3D&md5=d98e0efa4557aa6f9f8a5a7036aef265CAS |

Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Analytical Biochemistry 25, 192–205.
Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF1MXhslCktA%3D%3D&md5=15c61c8b8df7b8bfea8627a8caeb9759CAS | 4973948PubMed |

Speijers GJA, van de Brandt PA (2003) ‘Nitrate (and potential endogenous formation of N-nitroso compounds).’ (World Health Organization: Geneva)

Tabatabai MA, Bremner JM (1970) Arylsulfatase activity of soils. Soil Science Society of America Journal 34, 225–229.
Arylsulfatase activity of soils.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE3cXktVejsrg%3D&md5=aac5b197104620f2c279eb20531a8010CAS |

Takahashi H, Watanabe-Takahashi A, Smith FW, Blake-Kalff M, Hawkesford MJ, Saito K (2000) The role of three functional sulphate transporters involved in uptake and translocation of sulphate in Arabidopsis thaliana. The Plant Journal 23, 171–182.
The role of three functional sulphate transporters involved in uptake and translocation of sulphate in Arabidopsis thaliana.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXmtFOns7g%3D&md5=b5fc9cdbdae0607475f6ee343f0a0118CAS | 10929111PubMed |

Terry N, Zayed AM, de Souza MP, Tarun AS (2000) Selenium in higher plants. Annual Review of Plant Physiology and Plant Molecular Biology 51, 401–432.
Selenium in higher plants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXlsVymt70%3D&md5=570ae1d9204104f41b434014b4ab4e10CAS | 15012198PubMed |

Titmarsh IJ, Doughton J, Woods E (2010) Agronomy looking forward, thinking broadly. Crop & Pasture Science 61, 522–527.
Agronomy looking forward, thinking broadly.Crossref | GoogleScholarGoogle Scholar |

Tomasi N, Mimmo T, Terzano R, Alfeld M, Janssens K, Zanin L, Pinton R, Varanini Z, Cesco S (2014) Nutrient accumulation in leaves of Fe-deficient cucumber plants treated with natural Fe complexes. Biology and Fertility of Soils 50, 973–982.
Nutrient accumulation in leaves of Fe-deficient cucumber plants treated with natural Fe complexes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXmvFCksbo%3D&md5=a51dcbf5195483ed90085b8a810706d7CAS |

Turlo J, Gutkowska E, Malinowska E (2007) Relationship between the selenium, selenomethionine, and selenocysteine content of submerged cultivated mycelium of Lentinula edodes. Acta Chromatographica 18, 36–48.

Van Hoewyk D (2013) A tale of two toxicities: malformed selenoproteins and oxidative stress both contribute to selenium stress in plants. Annals of Botany 112, 965–972.
A tale of two toxicities: malformed selenoproteins and oxidative stress both contribute to selenium stress in plants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsFyns7rJ&md5=f5191ce1a75c953bd7772e69ebbad709CAS | 23904445PubMed |

Van Hoewyk D, Garifullina GF, Ackley AR, Abdel-Ghany SE, Marcus MA, Fakra S, Ishiyama K, Inoue E, Pilon M, Takahashi H, Pilon-Smits EAH (2005) Overexpression of AtCpNifS enhances selenium tolerance and accumulation in Arabidopsis. Plant Physiology 139, 1518–1528.
Overexpression of AtCpNifS enhances selenium tolerance and accumulation in Arabidopsis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXht1Ogu7rM&md5=a42377766cfa6e65884e0ae3703535b3CAS | 16244144PubMed |

Van Hoewyk D, Takahashi H, Inoue E, Hess A, Tamaoki M, Pilon-Smits EAH (2008) Transcriptome analyses give insights into selenium-stress responses and selenium tolerance mechanisms in Arabidopsis. Physiologia Plantarum 132, 236–253.

White PJ, Broadley MR (2009) Biofortification of crops with seven mineral elements often lacking in human diets – iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytologist 182, 49–84.
Biofortification of crops with seven mineral elements often lacking in human diets – iron, zinc, copper, calcium, magnesium, selenium and iodine.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXksVKhtbw%3D&md5=bc00d739e8f944c687ed9fe0c40fb43eCAS | 19192191PubMed |

White PJ, Bowen HC, Parmaguru P, Fritz M, Spracklen WP, Spiby RE, Meacham MC, Mead A, Harriman M, Trueman LJ, Smith BM, Thomas B, Broadley MR (2004) Interactions between selenium and sulphur nutrition in Arabidopsis thaliana. Journal of Experimental Botany 55, 1927–1937.
Interactions between selenium and sulphur nutrition in Arabidopsis thaliana.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXntFais7w%3D&md5=a45996ce7fe110bf6f57741c5e8bf7efCAS | 15258164PubMed |

Yoshimoto N, Takahashi H, Smith FW, Yamaya T, Saito K (2002) Two distinct high-affinity sulfate transporters with different inducibilities mediate uptake of sulfate in Arabidopsis roots. The Plant Journal 29, 465–473.
Two distinct high-affinity sulfate transporters with different inducibilities mediate uptake of sulfate in Arabidopsis roots.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XisVags74%3D&md5=a08ac8e063fc4beba931415f59593346CAS | 11846879PubMed |

Zancan S, Cesco S, Ghisi S (2006) Effect of UV-B radiation on iron content and distribution in maize plants. Environmental and Experimental Botany 55, 266–272.
Effect of UV-B radiation on iron content and distribution in maize plants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtlWhsLnL&md5=3b254f7dda5fc191e91bf91454be051aCAS |

Zhao FJ, Su YH, Dunham SJ, Rakszegi M, Bedo Z, McGrath SP, Shewry PR (2009) Variation in mineral micronutrient concentrations in grain of wheat lines of diverse origin. Journal of Cereal Science 49, 290–295.
Variation in mineral micronutrient concentrations in grain of wheat lines of diverse origin.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXivVWlu7g%3D&md5=c7b807cc13bc1e6d8a1b6c63271befd9CAS |

Zhu YG, Pilon-Smits EAH, Zhao FJ, Williams PN, Meharg AA (2009) Selenium in higher plants: understanding mechanisms for biofortification and phytoremediation. Trends in Plant Science 14, 436–442.
Selenium in higher plants: understanding mechanisms for biofortification and phytoremediation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtVSntb7J&md5=590a93ceb722899c498dec9552951181CAS | 19665422PubMed |