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RESEARCH ARTICLE

Host resistance to Sclerotinia stem rot in historic and current Brassica napus and B. juncea varieties: critical management implications

Margaret B. Uloth A B , Ming Pei You A B and Martin J. Barbetti A B C
+ Author Affiliations
- Author Affiliations

A School of Plant Biology, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

B The UWA Institute of Agriculture, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

C Corresponding author. Email: martin.barbetti@uwa.edu.au

Crop and Pasture Science 66(8) 841-848 https://doi.org/10.1071/CP15064
Submitted: 17 February 2015  Accepted: 30 June 2015   Published: 31 July 2015

Abstract

Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum, is an important disease of oilseed brassicas, yet the susceptibility of Australian varieties is unknown. Fifty-five historic, current and potential new Australian canola and mustard varieties were field-screened to determine their relative levels of resistance to SSR. Mean lesion length following stem inoculation with a highly virulent isolate (MBRS1) of the prevailing S. sclerotiorum pathotype (76) ranged from 3.0 mm in the B. napus cultivar Mystic to 202.6 mm (P < 0.001). Three recently developed B. juncea varieties or breeding lines, Sahara, JB0T-908982 and Xceed X121 CL, were extremely susceptible to S. sclerotiorum (mean lesion lengths 90.6, 132.3 and 202.6 mm, respectively). Histological study showed that the high level of resistance in Mystic was associated with strong deposition of lignin in stem cortical cell walls to form a barrier between the invading pathogen and the vascular tissues. Lack of association between mean lesion length and the year of varietal release (R2 = 0.005) shows that there has been no improvement in level of resistance to SSR in Australian canola and mustard varieties over the last two decades. Although the very high susceptibility of a few B. juncea varieties demonstrated the value of SSR resistance present in B. napus varieties, this level of resistance is inadequate to prevent ongoing, severe yield losses from SSR under conditions conducive for disease development. Breeding programs can immediately utilise the SSR resistance in Mystic, and other recently identified resistances. This will enable a shift from the current dependence on fungicidal control to reliance on cost-effective, sustainable host resistance as the basis for better management of SSR.

Additional keywords: breeding, disease screening, mustard, oilseed rape, Sclerotinia rot.


References

Barbetti MJ, Li CX, Garg H, Li H, Banga SK, Banga SS, Sandhu PS, Singh R, Singh D, Liu SY, Gurung AM, Salisbury PA (2011) Host resistance in oilseed Brassicas against Sclerotinia—renewed hope for managing a recalcitrant pathogen. In ‘Proceedings of the 13th International Rapeseed Congress’. June 2011, Prague, Czech Republic. pp. 713–715. (GCIRC: Paris, France)

Barbetti MJ, Banga SS, Salisbury PA (2012) Challenges for crop production and management from pathogen biodiversity and diseases under current and future climate scenarios - case study with oilseed Brassicas. Field Crops Research 127, 225–240.
Challenges for crop production and management from pathogen biodiversity and diseases under current and future climate scenarios - case study with oilseed Brassicas.Crossref | GoogleScholarGoogle Scholar |

Barbetti MJ, Uloth M, You MP, Liu SY, Banga SS (2013) Sclerotinia on oilseed, forage and vegetable cruciferous crops—no longer an impossible disease to manage. In ‘Proceedings of the 15th International Sclerotinia Workshop’. 20–24 August 2013, Wuhan, China. p. 62. (Huazhong Agricultural University: Wuhan, China)

Barbetti MJ, Banga SK, Fu TD, Li YC, Singh D, Liu SY, Ge XT, Banga SS (2014) Comparative genotype reactions to Sclerotinia sclerotiorum within breeding populations of Brassica napus and B. juncea from India and China. Euphytica 197, 47–59.
Comparative genotype reactions to Sclerotinia sclerotiorum within breeding populations of Brassica napus and B. juncea from India and China.Crossref | GoogleScholarGoogle Scholar |

Brunin B (1972) Recherches sur la maladie du colza due a Leptosphaeria maculans (Desm.) Ces. and de Not. III.—Aspects anatomiques de la necrose du collet. Annals of Phytopathologie 4, 87–96.

Bucat J (2015) ‘Canola variety guide for 2015 cropping season.’ (Department of Agriculture and Food Western Australia: South Perth, W. Aust.) Available at: www.agric.wa.gov.au/news/media-releases/canola-variety-guide-available-2015-cropping-season

Buchwaldt L, Li R, Hegedus DD, Rimmer SR (2005) Pathogenesis of Sclerotinia sclerotiorum in relation to screening for resistance. In ‘Proceedings of the 13th International Sclerotinia Workshop’. Monterey, CA, USA. p. 22. (International Sclerotinia Working Group)

Burton W, Salisbury P, Potts D (2003) The potential of canola quality Brassica juncea as an oilseed crop for Australia In ‘Proceedings of the 13th Australian Research Assembly on Brassicas’. Tamworth, NSW. (Ed. J Edwards) pp. 62–64. (NSW Agriculture: Tamworth, NSW)

Cowling WA (2007) Genetic diversity in Australian canola and implications for crop breeding for changing future environments. Field Crops Research 104, 103–111.
Genetic diversity in Australian canola and implications for crop breeding for changing future environments.Crossref | GoogleScholarGoogle Scholar |

Delourme R, Barbetti MJ, Snowdon R, Zhao J, Manzanares-Dauleux M (2011) Genetics and genomics of resistance. In ‘Genetics, genomics and breeding of oilseed Brassicas’. (Eds D Edwards, J Batley, IAP Parkin, C Kole) pp. 276–318. (Science Publishers, CRC Press: Boca Raton, FL, USA)

Garg H, Sivasithamparam K, Banga SS, Barbetti MJ (2008) Cotyledon assay as a rapid and reliable method of screening for resistance against Sclerotinia sclerotiorum in Brassica napus genotypes. Australasian Plant Pathology 37, 106–111.
Cotyledon assay as a rapid and reliable method of screening for resistance against Sclerotinia sclerotiorum in Brassica napus genotypes.Crossref | GoogleScholarGoogle Scholar |

Garg H, Atri C, Sandhu PS, Kaur B, Renton M, Banga SK, Singh H, Singh C, Barbetti MJ, Banga SS (2010a) High level of resistance to Sclerotinia sclerotiorum in introgression lines derived from hybridization between wild crucifers and the crop Brassica species B. napus and B. juncea. Field Crops Research 117, 51–58.
High level of resistance to Sclerotinia sclerotiorum in introgression lines derived from hybridization between wild crucifers and the crop Brassica species B. napus and B. juncea.Crossref | GoogleScholarGoogle Scholar |

Garg H, Kohn LM, Andrew M, Li H, Sivasithamparam K, Barbetti MJ (2010b) Pathogenicity of morphologically different isolates of Sclerotinia sclerotiorum with Brassica napus and B. juncea genotypes. European Journal of Plant Pathology 126, 305–315.
Pathogenicity of morphologically different isolates of Sclerotinia sclerotiorum with Brassica napus and B. juncea genotypes.Crossref | GoogleScholarGoogle Scholar |

Garg H, Li H, Sivasithamparam K, Barbetti MJ (2013) Differentially expressed proteins and associated histological and disease progression changes in cotyledon tissue of a resistant and susceptible genotype of Brassica napus infected with Sclerotinia sclerotiorum. PLoS One 8, e65205
Differentially expressed proteins and associated histological and disease progression changes in cotyledon tissue of a resistant and susceptible genotype of Brassica napus infected with Sclerotinia sclerotiorum.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtVanurrN&md5=dd0d1e3f310546cdc9525f288e0c863aCAS | 23776450PubMed |

Ge XT, Li YP, Wan ZJ, You MP, Finnegan PM, Banga SS, Sandhu PS, Garg H, Salisbury PA, Barbetti MJ (2012) Delineation of Sclerotinia sclerotiorum pathotypes using differential resistance responses on Brassica napus and B. juncea genotypes enables identification of resistance to prevailing pathotypes. Field Crops Research 127, 248–258.
Delineation of Sclerotinia sclerotiorum pathotypes using differential resistance responses on Brassica napus and B. juncea genotypes enables identification of resistance to prevailing pathotypes.Crossref | GoogleScholarGoogle Scholar |

Goyal P, Chahar M, Barbetti MJ, Liu SY, Chattopadhyay C (2011) Resistance to sclerotinia rot caused by Sclerotinia sclerotiorum in Brassica juncea and B. napus germplasm. Indian Journal of Plant Protection 39, 60–64.

GRDC (Grains Research and Development Corporation) (2013) Sclerotinia stem rot in canola. Fact Sheet. Grains Research and Development Corporation, Canberra, ACT. Available at: www.grdc.com.au/RSS/~/media/252C076002964C428E42B6593D934607.ashx

GRDC (Grains Research and Development Corporation) (2014) Revised spring blackleg management guide. Fact Sheet. Grains Research and Development Corporation, Canberra, ACT. Available at: www.australianoilseeds.com/__data/assets/pdf_file/0017/10196/Blackleg_Management_Guide_SPRING_edition_2014.pdf

Hammond KE, Lewis G (1987) Variation in stem infections caused by aggressive and non-aggressive isolates of Leptosphaeria maculans on Brassica napus var. oleifera. Plant Pathology 36, 53–65.
Variation in stem infections caused by aggressive and non-aggressive isolates of Leptosphaeria maculans on Brassica napus var. oleifera.Crossref | GoogleScholarGoogle Scholar |

Hind-Lanoiselet T, Lewington F (2004) Canola concepts: managing Sclerotinia. Agnote DPI-490. New South Wales Department of Agriculture, Orange, NSW.

Hind-Lanoiselet T, Lewington F, Lindbeck K (2008) Managing sclerotinia stem rot in canola. GRDC/CAA disease management guide. Australian Oilseeds Federation. Available at: www.australianoilseeds.com/__data/assets/pdf_file/0018/5373/scleroweb_08.pdf (accessed 8 January 2015)

Ji R-Q, Dong X-B, Feng H, Barbetti MJ, Gao R-C, Liu Y-Y, Liu S-Y (2009) Expression of PDF1.2 in oxalate oxidase transgenic oilseed rape (Brassica napus L.). Plant Physiology Communication 45, 479–482.

Khangura R, Van Burgel A, Salam M, Aberra M, MacLeod WJ (2014) Why Sclerotinia was so bad in 2013? Understanding the disease and management options. In ‘Proceedings of the 2014 Crop Updates’. 24–25 February 2014, Perth, W. Aust. (Grain Industry Association of Western Australia: Perth, W. Aust.) Available at: www.giwa.org.au/pdfs/2014/Presented_Papers/Khangura%20et%20al%20presentation%20paper%20CU2014%20-DR.pdf

Li CX, Li H, Sivasithamparam K, Fu TD, Li YC, Liu SY, Barbetti MJ (2006) Expression of field resistance under Western Australian conditions to Sclerotinia sclerotiorum in Chinese and Australian Brassica napus and Brassica juncea germplasm and its relation with stem diameter. Australian Journal of Agricultural Research 57, 1131–1135.
Expression of field resistance under Western Australian conditions to Sclerotinia sclerotiorum in Chinese and Australian Brassica napus and Brassica juncea germplasm and its relation with stem diameter.Crossref | GoogleScholarGoogle Scholar |

Li CX, Li H, Siddique AB, Sivasithamparam K, Salisbury P, Banga SS, Banga SK, Chattopadhyay C, Kumar A, Singh R, Singh D, Agnihotri A, Liu SY, Li YC, Tu J, Fu TD, Wang YF, Barbetti MJ (2007a) The importance of the type and time of inoculation and assessment in the determination of resistance in Brassia napus and B. juncea to Sclerotinia sclerotiorum. Australian Journal of Agricultural Research 58, 1198–1203.
The importance of the type and time of inoculation and assessment in the determination of resistance in Brassia napus and B. juncea to Sclerotinia sclerotiorum.Crossref | GoogleScholarGoogle Scholar |

Li H, Kuo J, Barbetti MJ, Sivasithamparam K (2007b) Differences in the responses of stem tissues of spring-type Brassica napus cultivars with polygenic resistance and single dominant gene-based resistance to inoculation with Leptosphaeria maculans. Canadian Journal of Botany 85, 191–203.
Differences in the responses of stem tissues of spring-type Brassica napus cultivars with polygenic resistance and single dominant gene-based resistance to inoculation with Leptosphaeria maculans.Crossref | GoogleScholarGoogle Scholar |

Li CX, Liu SY, Sivasithamparam K, Barbetti MJ (2009) New sources of resistance to Sclerotinia stem rot caused by Sclerotinia sclerotiorum in Chinese and Australian Brassica napus and Brassica juncea germplasm screened under Western Australian conditions. Australasian Plant Pathology 38, 149–152.
New sources of resistance to Sclerotinia stem rot caused by Sclerotinia sclerotiorum in Chinese and Australian Brassica napus and Brassica juncea germplasm screened under Western Australian conditions.Crossref | GoogleScholarGoogle Scholar |

Navabi ZK, Strelkov SE, Good AG, Thiagarajah MR, Rahman MH (2010) Brassica B-genome resistance to stem rot (Sclerotinia sclerotiorum) in a doubled haploid population of Brassica napus × Brassica carinata. Canadian Journal of Plant Pathology 32, 237–246.
Brassica B-genome resistance to stem rot (Sclerotinia sclerotiorum) in a doubled haploid population of Brassica napus × Brassica carinata.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXmvFOqtLs%3D&md5=2e60db6944db32665d31f66db24c8e47CAS |

Oram RN, Kirk JTO, Veness PE, Hurlstone CJ, Edlington JP, Halsall DM (2005) Breeding Indian mustard [Brassica juncea (L) Czern] for cold-pressed edible oil production – a review. Australian Journal of Agricultural Research 56, 581–596.
Breeding Indian mustard [Brassica juncea (L) Czern] for cold-pressed edible oil production – a review.Crossref | GoogleScholarGoogle Scholar |

Salisbury P, Luckett D (2010) Global search for new germplasm. Ground Cover—Oilseed Breeding Supplement 85. Grains Research and Development Corporation, Canberra, ACT. Available at: www.grdc.com.au/Media-Centre/Ground-Cover-Supplements/Ground-Cover-Issue-85-Oilseed-Breeding-Supplement/global-search-for-new-germplasm.

Singh R, Singh D, Li H, Sivasithamparam K, Yadav NR, Salisbury P, Barbetti MJ (2008a) Management of Sclerotinia rot of oilseed Brassicas – a focus on India. Brassica 10, 1–27.

Singh R, Singh D, Barbetti MJ, Singh H, Li CX, Sivasithamparam K, Salisbury P, Burton W, Fu T (2008b) Sclerotinia rot tolerance in oilseed Brassica. Journal of Oilseeds Research 25, 223–225.

Singh R, Singh D, Salisbury P, Barbetti MJ (2010) Field evaluation of Indian and exotic oilseed Brassica napus and B. juncea germplasm against Sclerotinia stem rot. Indian Journal of Agricultural Science 80, 1067–1071.

Uloth MB, You MP, Finnegan PM, Banga SS, Banga SK, Sandhu PS, Yi H, Salisbury PA, Barbetti MJ (2013) New sources of resistance to Sclerotinia sclerotiorum for crucifer crops. Field Crops Research 154, 40–52.
New sources of resistance to Sclerotinia sclerotiorum for crucifer crops.Crossref | GoogleScholarGoogle Scholar |

Uloth MB, You MP, Finnegan PM, Banga SS, Yi H, Barbetti MJ (2014) Seedling resistance to Sclerotinia sclerotiorum as expressed across diverse cruciferous species. Plant Disease 98, 184–190.
Seedling resistance to Sclerotinia sclerotiorum as expressed across diverse cruciferous species.Crossref | GoogleScholarGoogle Scholar |

Uloth M, Clode PL, You MP, Cawthray G, Barbetti MJ (2015b) Temperature adaptation in Sclerotinia sclerotiorum affects its ability to infect Brassica carinata. Plant Pathology
Temperature adaptation in Sclerotinia sclerotiorum affects its ability to infect Brassica carinata.Crossref | GoogleScholarGoogle Scholar |

Wang J, Kaur S, Cogan NOI, Dobrowolski MP, Salisbury PA, Burton WA, Baillie R, Hand M, Hopkins C, Forster JW, Smith KF, Spangenberg G (2009) Assessment of genetic diversity in Australian canola (Brassica napus L.) cultivars using SSR markers. Crop & Pasture Science 60, 1193–1201.
Assessment of genetic diversity in Australian canola (Brassica napus L.) cultivars using SSR markers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsVKhtL%2FJ&md5=287fd610136b393a2f57e71b378ea568CAS |

Ware A (2015) Canola variety sowing guide 2015. In ‘2015 South Australian Research and Development Institute Sowing Guide’. pp. 36–40. (SARDI: Adelaide, S. Aust.) Available at: www.google.com.au/#q=Ware+A+(2015)+Canola+variety+sowing+guide+2015

Wu J, Cai G, Tu J, Li L, Liu S, Luo X, Zhou L, Fan C, Zhou Y (2013) Identification of QTLs for resistance to Sclerotinia Stem Ror and BnaC.IGMT5.a as a candidate gene of the major resistant QTL SRC6 in Brassica napus. PLoS One 8, e67740
Identification of QTLs for resistance to Sclerotinia Stem Ror and BnaC.IGMT5.a as a candidate gene of the major resistant QTL SRC6 in Brassica napus.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtFGjsLfI&md5=fbc36270aaa742e5ac35770eb39ba376CAS | 23844081PubMed |

Zhao J, Meng J (2003) Genetic analysis of loci associated with partial resistance to Sclerotinia sclerotiorum in rapeseed (Brassica napus L.). Theoretical and Applied Genetics 106, 759–764.

Zhao J, Peltier AJ, Meng J, Osborn TC, Grau CR (2004) Evaluation of Sclerotinia stem rot resistance in oilseed Brassica napus using a petiole inoculation technique under greenhouse conditions. Plant Disease 88, 1033–1039.
Evaluation of Sclerotinia stem rot resistance in oilseed Brassica napus using a petiole inoculation technique under greenhouse conditions.Crossref | GoogleScholarGoogle Scholar |

Zhao J, Udall J, Quijada P, Grau C, Meng J, Osborn T (2006) Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L. Theoretical and Applied Genetics 112, 509–516.
Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xnt1yjsLo%3D&md5=f2786be549eaf203c14f82eb87bdba4eCAS | 16333614PubMed |