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Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Hexose uptake by developing cotyledons of Vicia faba: physiological evidence for transporters of differing affinities and specificities

Gregory N. Harrington A B , Katherine E. Dibley A , Raymond J. Ritchie C , Christina E. Offler A and John W. Patrick A D
+ Author Affiliations
- Author Affiliations

A School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW 2308, Australia.

B Present address: Department of Integrated Natural Sciences, Arizona State University, at the West campus, Glendale, AZ 85306, USA.

C Biology A-08, University of Sydney, Sydney, NSW 2006, Australia.

D Corresponding author. Email: john.patrick@newcastle.edu.au

Functional Plant Biology 32(11) 987-995 https://doi.org/10.1071/FP05081
Submitted: 4 April 2005  Accepted: 31 May 2005   Published: 28 October 2005

Abstract

Cotyledons of broad bean (Vicia faba L.) develop in an apoplasmic environment that shifts in composition from one dominated by hexoses to one dominated by sucrose. During the latter phase of development, sucrose / H+ symporter activity and expression is restricted to cotyledon epidermal transfer cell complexes that support sucrose fluxes that are 8.5-fold higher than those exhibited by the storage parenchyma. In contrast, the flux difference between these cotyledon tissues is only 1.7-fold for hexoses. Glucose and fructose uptake was shown to be sensitive to PCMBS and phloridzin, both of which slow H+-sugar transport. A low Km (or high affinity transporter, HAT) mechanism transports glucose and glucose-analogues exclusively. No HAT system for fructose could be found. A high Km (low affinity transporter, LAT) mechanism transports a broader range of hexoses, including glucose and fructose. Consistent with glucose and fructose transport being H+-coupled, their uptake was inhibited by dissipating the proton motive force (pmf) by treating cotyledons with carbonyl cyanide m-chlorophenol hydrazone, propionic acid or tetraphenylphosphonium ion. Erythrosin B inhibited hexose uptake, indicating a role for the P-type H+-ATPase in establishing the pmf. It is concluded that H+-coupled glucose and fructose transport mechanisms occur at plasma membranes of dermal transfer cell complexes and storage parenchyma cells. These transport mechanisms are active during pre- and storage phases of cotyledon development. However, hexose symport only makes a quantitative contribution to cotyledon biomass gain during the pre-storage stage of development.

Keywords: fructose; glucose; hexose; plasma membrane transport; seed development; Vicia faba cotyledons.


Acknowledgments

This study was supported by a large Australian Research Council grant awarded to JWP and CEO. GNH is grateful for the support offered by an Australian Postgraduate Research Award. We are indebted to Louise Hetherington for technical assistance and Mr Kevin Stokes for a continuous supply of healthy plant material.


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