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Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

High temperature enhances inhibitor production but reduces fallover in tobacco Rubisco

Stephen M. Schrader A D , Heather J. Kane B , Thomas D. Sharkey C and Susanne von Caemmerer B
+ Author Affiliations
- Author Affiliations

A Photosynthesis Research Unit, Agricultural Research Service, United States Department of Agriculture, 1201 W. Gregory Dr., Urbana, IL 61801, USA.

B Molecular Plant Physiology, Research School of Biological Sciences, Australian National University, GPO Box 475, Canberra, ACT 2601, Australia.

C Department of Botany, University of Wisconsin — Madison, 430 Lincoln Drive, Madison, WI 53706, USA.

D Corresponding author. Email: Schrader@uiuc.edu

Functional Plant Biology 33(10) 921-929 https://doi.org/10.1071/FP06059
Submitted: 20 March 2006  Accepted: 24 May 2006   Published: 2 October 2006

Abstract

High temperature inhibits photosynthesis by several mechanisms including reduction in Rubisco activity. While the initial reaction velocity of purified, fully carbamylated, inhibitor-free Rubisco increases with temperature in vitro, over time, the reaction velocity slowly declines (fallover) because of the enzymatic and non-enzymatic production of inhibitors from the substrate ribulose-1,5-bisphosphate. We tested whether fallover could contribute to the decline in Rubisco activity observed in leaf extracts at high temperature. Production of d-xylulose-1,5-bisphosphate (XuBP), an inhibitor of Rubisco, was greater at 35 and 45°C than at 25°C but fallover was less severe at 35 and 45°C than at 25°C, both in rate and extent under saturating CO2 and ambient O2. This apparent dichotomy is consistent with the catalytic site of Rubisco loosening at higher temperatures and releasing inhibitors more easily. The loosening of the catalytic site was supported by the observation that RuBP and XuBP were released from their complexes with uncarbamylated, Mg2+-free Rubisco faster at 35 and 45°C than at 25°C. We conclude that, although XuBP production increased relative to catalytic throughput at higher temperatures, this was more than compensated for by its faster release, resulting in less fallover inhibition at higher temperatures.

Keywords: fallover, heat stress, high temperature, Rubisco.


Acknowledgments

We thank Dr John Andrews for helpful advice and discussion during the course of this study. This work was supported by the USDA-CSREES, National Research Initiative grant no. 2004-35100-14860.


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