A survey of the natural variation in biomechanical and cell wall properties in inflorescence stems reveals new insights into the utility of Arabidopsis as a wood model
Colleen P. MacMillan A , Philip J. O’Donnell B , Anne-Marie Smit B , Rob Evans C , Zbigniew H. Stachurski D , Kirk Torr B , Mark West B , Jacqueline Baltunis A and Timothy J. Strabala B E
+ Author Affiliations
- Author Affiliations
A CSIRO Plant Industry, Canberra, ACT 2601, Australia.
B Scion, Rotorua 3046, New Zealand.
C CSIRO Materials Science and Engineering, Melbourne, Vic. 3168, Australia.
D College of Engineering, Australian National University, Canberra, ACT 0200, Australia.
E Corresponding author. Email: tim.strabala@scionresearch.com
Functional Plant Biology 40(7) 662-676 https://doi.org/10.1071/FP12386
Submitted: 20 December 2012 Accepted: 24 February 2013 Published: 5 April 2013
Abstract
The natural trait variation in Arabidopsis thaliana (L.) Heynh. accessions is an important resource for understanding many biological processes but it is underexploited for wood-related properties. Twelve A. thaliana accessions from diverse geographical locations were examined for variation in secondary growth, biomechanical properties, cell wall glycan content, cellulose microfibril angle (MFA) and flowering time. The effect of daylength was also examined. Secondary growth in rosette and inflorescence stems was observed in all accessions. Organised cellulose microfibrils in inflorescence stems were found in plants grown under long and short days. A substantial range of phenotypic variation was found in biochemical and wood-related biophysical characteristics, particularly for tensile strength, tensile stiffness, MFA and some cell wall components. The four monosaccharides galactose, arabinose, rhamnose and fucose strongly correlated with each other as well as with tensile strength and MFA, consistent with mutations in arabinogalactan protein and fucosyl- and xyloglucan galactosyl-transferase genes that result in decreases in strength. Conversely, these variables showed negative correlations with lignin content. Our data support the notion that large-scale natural variation studies of wood-related biomechanical and biochemical properties of inflorescence stems will be useful for the identification of novel genes important for wood formation and quality, and therefore biomaterial and renewable biofuel production.
Additional keywords: microfibril angle, neutral carbohydrate content, SilviScan, stiffness, tensile strength.
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