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Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
REVIEW

The structure and activity of nodulation-suppressing CLE peptide hormones of legumes

April H. Hastwell A , Peter M. Gresshoff A and Brett J. Ferguson A B
+ Author Affiliations
- Author Affiliations

A Centre for Integrative Legume Research, School of Agricultural and Food Sciences, The University of Queensland, St Lucia, Brisbane, Qld 4072, Australia.

B Corresponding author. Email: b.ferguson1@uq.edu.au

Functional Plant Biology 42(3) 229-238 https://doi.org/10.1071/FP14222
Submitted: 8 August 2014  Accepted: 3 November 2014   Published: 12 December 2014

Abstract

Legumes form a highly-regulated symbiotic relationship with specific soil bacteria known as rhizobia. This interaction results in the de novo formation of root organs called nodules, in which the rhizobia fix atmospheric di-nitrogen (N2) for the plant. Molecular mechanisms that regulate the nodulation process include the systemic ‘autoregulation of nodulation’ and the local nitrogen-regulation of nodulation pathways. Both pathways are mediated by novel peptide hormones called CLAVATA/ESR-related (CLE) peptides that act to suppress nodulation via negative feedback loops. The mature peptides are 12–13 amino acids in length and are post-translationally modified from the C-terminus of tripartite-domain prepropeptides. Structural redundancy between the prepropeptides exists; however, variations in external stimuli, timing of expression, tissue specificity and presence or absence of key functional domains enables them to act in a specific manner. To date, nodulation-regulating CLE peptides have been identified in Glycine max (L.) Merr., Medicago truncatula Gaertn., Lotus japonicus (Regel) K.Larsen and Phaseolus vulgaris L. One of the L. japonicus peptides, called LjCLE-RS2, has been structurally characterised and found to be an arabinosylated glycopeptide. All of the known nodulation CLE peptides act via an orthologous leucine rich repeat (LRR) receptor kinase. Perception of the peptide results in the production of a novel, unidentified inhibitor signal that acts to suppress further nodulation events. Here, we contrast and compare the various nodulation-suppressing CLE peptides of legumes.

Additional keywords: autoregulation of nodulation, legume nodulation, nitrate-regulation of nodulation, nodule, plant peptide signalling, symbiosis.


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