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Healthcare Infection Healthcare Infection Society
Official Journal of the Australasian College for Infection Prevention and Control
RESEARCH ARTICLE

Evaluation of the relationship between ATP bioluminescence assay and the presence of organisms associated with healthcare-associated infections

Shawn G. Gibbs A F , Harlan Sayles B , Oleg Chaika A , Angela Hewlett C , Erica M. Colbert A and Philip W. Smith C D E
+ Author Affiliations
- Author Affiliations

A Department of Environmental, Agricultural and Occupational Health, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska, USA.

B Department of Biostatistics, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska, USA.

C Section of Infectious Disease, College of Medicine, University of Nebraska Medical Center, Omaha, Nebraska, USA.

D Center for Preparedness Education, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska, USA.

E Department of Epidemiology, College of Public Health, University of Nebraska Medical Center, Omaha, Nebraska, USA.

F Corresponding author. Email: sgibbs@unmc.edu

Healthcare Infection 19(3) 101-107 https://doi.org/10.1071/HI14010
Submitted: 9 April 2014  Accepted: 5 June 2014   Published: 26 June 2014

Abstract

Background: High prevalence and high mortality rates associated with healthcare-associated Infections (HAI) indicate there is a need to prevent HAIs from spreading. Cleaning and disinfection of hospital surfaces are fundamental to preventing HAIs, as is the confirmation of the success of these processes. Adenosine triphosphate bioluminescence has been identified as a quicker way to confirm cleaning, but questions remain regarding its specificity regarding microorganisms important to HAIs.

Methods: This study evaluated ATP bioluminescence’s efficacy in determining microbial contamination on 17 surfaces from the healthcare environment, and to determine if the ATP measurements of Acinetobacter baumannii, Candida albicans, Enterococcus faecalis, Escherichia coli, Mycobacterium smegmatis, and methicillin-resistant Staphylococcus aureus corresponded to quantitative microbiology.

Results: A strong positive correlation was discovered for each of the six organisms associated with HAIs, as well as an additional ‘all organisms’ analysis that combined all the six organisms.

Conclusion: This study demonstrated a correlation between ATP bioluminescence measurements and quantitative microbiology; however, it was not as strong at low bacterial concentrations.

Additional keywords: Acinetobacter baumannii, Adenosine triphosphate (ATP), ATP measurement, Candida albicans, Enterococcus faecalis, Escherichia coli, healthcare-associated infections, Mycobacterium smegmatis, and methicillin-resistant Staphylococcus aureus.


References

[1]  Klevens RM, Edwards R, Richards CL, Horan TC, Gaynes RP, Pollock DA, et al Estimating health care-associated infections and deaths in U.S. hospitals, 2002. Public Health Rep 2007; 122 160–6.
| 17357358PubMed |

[2]  Amodio E, Cannova L, Villfrate MR, Merendino AM, Aprea L, Calamusa G. Analytical performance issues comparison of ATP bioluminescence and aerobic bacterial count for evaluating surface cleanliness in an Italian hospital. J Occup Environ Hyg 2014; 11 D23–7.
Analytical performance issues comparison of ATP bioluminescence and aerobic bacterial count for evaluating surface cleanliness in an Italian hospital.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhs1ektLzJ&md5=62316be794f65d4bfddaa5a1cd04dcb5CAS | 24369935PubMed |

[3]  Boyce JM, Havill NL, Havill HL, Mangione E, Dumigan DG, Moore BA. Comparison of fluorescent marker systems with 2 quantitative methods of assessing terminal cleaning practices. Infect Control Hosp Epidemiol 2011; 32 1187–93.
Comparison of fluorescent marker systems with 2 quantitative methods of assessing terminal cleaning practices.Crossref | GoogleScholarGoogle Scholar | 22080657PubMed |

[4]  Aiken ZA, Wilson M, Pratten J. Evaluation of ATP bioluminescence assays for potential use in a hospital setting. Infect Control Hosp Epidemiol 2011; 32 507–9.
Evaluation of ATP bioluminescence assays for potential use in a hospital setting.Crossref | GoogleScholarGoogle Scholar | 21515983PubMed |

[5]  Griffith CJ, Cooper RA, Gilmore J, Davies C, Lewis M. An evaluation of hospital cleaning regimes and standards. J Hosp Infect 2000; 45 19–28.
An evaluation of hospital cleaning regimes and standards.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3c3psFWmsQ%3D%3D&md5=56d744ac93c16af8c7158f75ebdc02e9CAS | 10833340PubMed |

[6]  Shama G, Malik DJ. The uses and abuses of rapid bioluminescence-based ATP assays. Int J Hyg Environ Health 2013; 216 115–25.
The uses and abuses of rapid bioluminescence-based ATP assays.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XmtFGnsb0%3D&md5=3d90bee4cf9ece6136b71058f20900feCAS | 22541898PubMed |

[7]  Smith PW, Sayles H, Hewlett A, Cavalieri RJ, Gibbs SG, Rupp ME. A study of three methods for assessment of hospital environmental cleaning. Healthc Infect 2013; 18 80–5.
A study of three methods for assessment of hospital environmental cleaning.Crossref | GoogleScholarGoogle Scholar |

[8]  Mulvey D, Redding P, Roberston C, Woodall C, Kingsmore P, Bedwell D, et al Finding a benchmark for monitoring hospital cleanliness. J Hosp Infect 2011; 77 25–30.
Finding a benchmark for monitoring hospital cleanliness.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3M%2FmvVGgsg%3D%3D&md5=2c4766cd4244df3b0b19b2f23acab7d0CAS | 21129820PubMed |

[9]  Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA. Manual of Clinical Microbiology. 9th edn. Washington, DC: American Society for Microbiology; 2007.

[10]  Guh A, Carling P. Options for Evaluating Environmental Cleaning. Available from: http://www.cdc.gov/hai/toolkits/evaluating-environmental-cleaning.html [verified June 2014].

[11]  Smith PW, Gibbs SG, Sayles H, Hewlett A, Rupp ME, Iwen PC. Observations on hospital room contamination testing. Healthc Infect 2013; 18 10–3.
Observations on hospital room contamination testing.Crossref | GoogleScholarGoogle Scholar |

[12]  Huslage K, Rutala WA, Sickber-Bennett E, Weber DJ. A quantitative approach to defining ‘high-touch’ surfaces in hospitals. Infect Control Hosp Epidemiol 2010; 31 850–3.
A quantitative approach to defining ‘high-touch’ surfaces in hospitals.Crossref | GoogleScholarGoogle Scholar | 20569115PubMed |

[13]  Whiteley GS, Derry C, Glasbey T. The comparative performance of three brands of portable ATP-bioluminometer intended for use in hospital infection control. Healthc Infect 2012; 17 91–7.
The comparative performance of three brands of portable ATP-bioluminometer intended for use in hospital infection control.Crossref | GoogleScholarGoogle Scholar |

[14]  Whiteley GS, Derry C, Glasbey T. The comparative performance of three brands of portable ATP-bioluminometer intended for use in hospital infection control. Infect Control Hosp Epidemiol 2013; 34 538–40.
The comparative performance of three brands of portable ATP-bioluminometer intended for use in hospital infection control.Crossref | GoogleScholarGoogle Scholar | 23571377PubMed |