CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Marine & Freshwater Research   
Marine & Freshwater Research
Journal Banner
  Advances in the Aquatic Sciences
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
Content
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Sample Issue
For Authors
General Information
Instructions to Authors
Submit Article
Open Access
For Referees
General Information
Review an Article
Referee Guidelines
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our Email Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter youtube

 

Article << Previous     |     Next >>   Contents Vol 51(8)

Use of FDA and flow cytometry to assess metabolic activity as an indicator of nutrient status in phytoplankton

Justin D. Brookes, Sean M. Geary, George G. Ganf and Michael D. Burch

Marine and Freshwater Research 51(8) 817 - 823
Published: 2000

Abstract

This study reports the use of a technique to determine nutrient limitation of cultured and natural phytoplankton. The technique, an FDA-activity assay, which is usually used to assess cell viability, was used to measure metabolic activity in response to nutrient addition; the metabolic activity of phytoplankton was determined as the rate of hydrolysis of fluorescein diacetate (FDA), by intracellular esterases, to fluorescein, which was detectedusing a flow cytometer. Replacement of the limiting nutrient to nitrogen- or phosphorus-limited cultures and field populations resulted in an increase in metabolic activity that was detectable 24 h after nutrient addition. By flow cytometry, the natural phytoplankton community can be divided into different taxonomic groups; the response of these to FDA could be determined individually to allow identification of the nutrients limiting each type of phytoplankton. This would be more specific than the assessment of a whole-community response, which may mask subtle differences among taxa.



Full text doi:10.1071/MF00048

© CSIRO 2000

blank image
Subscriber Login
Username:
Password:  

 
PDF (558 KB) $25
 Export Citation
 Print
  
    
Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2015