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Plant function and evolutionary biology
RESEARCH ARTICLE

Heat-Induced Alterations in Thylakoid Membrane Protein Composition in Barley

TS Takeuchi and JP Thornber

Australian Journal of Plant Physiology 21(6) 759 - 770
Published: 1994

Abstract

Biochemical and spectroscopic studies on the effects of high temperatures (45-47º C) over a 1 h period on the protein composition, fluorescence and photochemical activities of the barley thylakoid membrane were made. Photosystem II (PS II) activity decreased as expected, and photosystem I (PS I) activity also unexpectedly decreased. Our data support previous conclusions that the decrease in PS I activity is largely due to inactivation (or loss) of a component between the two photosystems. A two-dimensional electrophoretic system permitted first the separation of the thylakoid pigment-protein complexes of unstressed and stressed plants, followed by a determination of their subunit composition. The changes in the protein composition of each pigment-protein complex in response to elevated temperatures were monitored. Heat changed the quaternary structure of PS II and resulted in removal of the oxygen-evolving enhancer proteins from the thylakoid, but did essentially no damage to the PS I complex. The PS II core complex dissociated from a dimeric form to a monomeric one, and the major LHC II component (LHC IIb) changed from a trimeric to a monomeric form. The pigments that are lost from thylakoids during heat stress are mainly removed from the PS II pigment-proteins.

https://doi.org/10.1071/PP9940759

© CSIRO 1994

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