CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Functional Plant Biology   
Functional Plant Biology
Journal Banner
  Plant Function & Evolutionary Biology
blank image Search
blank image blank image
blank image
  Advanced Search

Journal Home
About the Journal
Editorial Structure
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Evolutionary Reviews
Sample Issue
Call for Papers
For Authors
General Information
Submit Article
Author Instructions
Open Access
Awards and Prizes
For Referees
Referee Guidelines
Review an Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our Email Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter logo LinkedIn

red arrow PrometheusWiki
blank image
Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 23(4)

Transformation of Wheat With the Gene Encoding the Coat Protein of Barley Yellow Mosaic Virus

S Karunaratne, A Sohn, A Mouradov, J Scott, HH Steinbiss and KJ Scott

Australian Journal of Plant Physiology 23(4) 429 - 435
Published: 1996


Wheat plants (Triticum aestivum cv. Hartog) were stably transformed with the bar gene and the gene encoding the barley yellow mosaic virus coat protein. Cultured immature wheat embryos were bombarded with tungsten particles coated with the pEmuPAT-cp construct. Fifteen regenerating 'PPT- resistant' plants were selected on medium containing phosphinothricin. Of these, 11 plants had both the bar and cp genes integrated into the wheat genome and two plants had only the bar gene. Transmission of the two genes to progeny of two independent plants was confirmed. The barley yellow mosaic virus coat protein was detected in both the parent and progeny plants; however, bar gene expression occurred only in the parent plants. Keywords: wheat, gene encoding viral coat protein, bar gene, gene expression, transformation

Full text doi:10.1071/PP9960429

© CSIRO 1996

blank image
Subscriber Login

PDF (532 KB) $25
 Export Citation
Legal & Privacy | Contact Us | Help


© CSIRO 1996-2015