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Relationship between adiponectin and fertility in the female pig

Danila B. Campos A C , Marcelo Albornoz B , Paula C. Papa C , Marie-France Palin D , Vilceu Bordignon B and Bruce D. Murphy A E

A Centre de recherche en reproduction animale, Faculté de médecine vétérinaire, Université de Montréal, CP 5000, St-Hyacinthe, Québec J2S 7C6, Canada.
B Department of Animal Science, McGill University, St-Anne de-Bellevue, Québec H9X 3V9, Canada.
C Departamento de Cirurgia, Setor de Anatomia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo – SP, 05508-270, Brasil.
D Dairy and Swine Research and Development Center, Agriculture and Agri-Food Canada, Lennoxville, Québec, J1M 1Z3, Canada.
E Corresponding author. Email: bruce.d.murphy@umontreal.ca

Reproduction, Fertility and Development - http://dx.doi.org/10.1071/RD13201
Submitted: 26 June 2013  Accepted: 26 November 2013   Published online: 13 January 2014


 
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Abstract

Adiponectin isoforms may mediate different aspects of the pleiotropic function of the protein, including the reproductive process. We examined the pattern of circulating adiponectin and adiponectin system expression in fat and ovarian tissues of hyperfertile and subfertile sows. We demonstrated the presence of five different isoforms of adiponectin (90, 158, 180, 250 and >250 kDa) in the circulation and identified a subgroup of subfertile females that displayed reduced abundance of all adiponectin isoforms as well as a lack of the 250-kDa adiponectin isoform in both serum and follicular fluid. Subfertility in these animals was associated with fewer large follicles and corpora lutea in the ovaries, as well as lower concentrations of 17β-oestradiol in the follicular fluid of large follicles. In addition, subfertile females showed higher adiponectin mRNA in fat tissue and altered mRNA and protein expression of adiponectin and its receptors in the ovary. Changes in the abundance and pattern of circulating adiponectin isoforms have been associated with reproductive disorders in animals and humans, including polycystic ovarian syndrome (PCOS). Our findings suggest that the adiponectin system may play an important role in controlling ovarian function and influencing porcine fertility.

Additional keywords: adiponectin isoforms, adiponectin receptors, ovary.


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