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Seminal plasma affects sperm sex sorting in boars

Diego V. Alkmin A, Inmaculada Parrilla A, Tatiana Tarantini A, David del Olmo A, Juan M. Vazquez A, Emilio A. Martinez A and Jordi Roca A B

A Department of Medicine and Animal Surgery, University of Murcia, Veterinary Medicine, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
B Corresponding author. Email: roca@um.es

Reproduction, Fertility and Development - http://dx.doi.org/10.1071/RD14088
Submitted: 11 March 2014  Accepted: 2 August 2014   Published online: 28 August 2014


 
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Abstract

Two experiments were conducted in boar semen samples to evaluate how both holding time (24 h) and the presence of seminal plasma (SP) before sorting affect sperm sortability and the ability of sex-sorted spermatozoa to tolerate liquid storage. Whole ejaculate samples were divided into three aliquots immediately after collection: one was diluted (1 : 1, v/v) in Beltsville thawing solution (BTS; 50% SP); the SP of the other two aliquots was removed and the sperm pellets were diluted with BTS + 10% of their own SP (10% SP) or BTS alone (0% SP). The three aliquots of each ejaculate were divided into two portions, one that was processed immediately for sorting and a second that was sorted after 24 h storage at 15–17°C. In the first experiment, the ability to exhibit well-defined X- and Y-chromosome-bearing sperm peaks (split) in the cytometry histogram and the subsequent sorting efficiency were assessed (20 ejaculates). In contrast with holding time, the SP proportion influenced the parameters examined, as evidenced by the higher number of ejaculates exhibiting split and better sorting efficiency (P < 0.05) in semen samples with 0–10% SP compared with those with 50% SP. In a second experiment, the quality (viability, total and progressive motility) and functionality (plasma membrane fluidity and intracellular generation of reactive oxygen species) of sex-sorted spermatozoa were evaluated after 0, 72 and 120 h storage at 15–17°C (10 ejaculates). Holding time and SP proportion did not influence the quality or functionality of stored sex-sorted spermatozoa. In conclusion, a holding time as long as 24 h before sorting did not negatively affect sex sorting efficiency or the ability of sorted boar spermatozoa to tolerate long-term liquid storage. A high proportion of SP (50%) in the semen samples before sorting reduced the number of ejaculates to be sorted and negatively influenced the sorting efficiency, but did not affect the ability of sex-sorted spermatozoa to tolerate liquid storage.

Additional keyword: holding time.


References

Aisen, E. G., Alvarez, H. L., Venturino, A., and Garde, J. J. (2000). Effect of trehalose and EDTA on cryoprotective action of ram semen diluents. Theriogenology 53, 1053–1061.
CrossRef | CAS | PubMed |

Alkmin, D. V., Parrilla, I., Tarantini, T., Parlapan, L., del Olmo, D., Vazquez, J. M., Martinez, E. A., and Roca, J. (2014). Intra- and inter-boar variability in flow cytometric sperm sex sorting. Theriogenology 82, 501–508.
CrossRef | PubMed |

Awda, B. J., Mackenzie-Bell, M., and Buhr, M. M. (2009). Reactive oxygen species and boar sperm function. Biol. Reprod. 81, 553–561.
CrossRef | CAS | PubMed |

Barrabes Aneas, S., Gary, B. G., and Bouvier, B. P. (2008). Collectis® automated boar collection technology. Theriogenology 70, 1368–1373.
CrossRef |

Burroughs, C. A., Graham, J. K., Lenz, R. W., and Seidel, G. E. (2013). Seminal plasma effects on sex-sorting bovine sperm. Theriogenology 79, 551–557.
CrossRef | CAS | PubMed |

Caballero, I., Vazquez, J. M., Mayor, G. M., Almiñana, C., Calvete, J. J., Sanz, L., and Martinez, E. A. (2009). PSP-I/PSP-II spermadhesin exert a decapacitation effect on highly extended boar spermatozoa. Int. J. Androl. 32, 505–513.
CrossRef | CAS | PubMed |

Cremades, T., Roca, J., Rodriguez-Martinez, H., Abaigar, T., Vazquez, J. M., and Martinez, E. A. (2005). Kinematic changes during the cryopreservation of boar spermatozoa. J. Androl. 26, 610–618.
CrossRef | PubMed |

de Graaf, S. P., Leahy, T., Marti, J., Evans, G., and Maxwell, W. M. (2008). Application of seminal plasma in sex-sorting and sperm cryopreservation. Theriogenology 70, 1360–1363.
CrossRef | CAS | PubMed |

del Olmo, D., Parrilla, I., Gil, M. A., Maside, C., Tarantini, T., Angel, M. A., Roca, J., Martinez, E. A., and Vazquez, J. M. (2013). Handling of boar spermatozoa during and after flow cytometric sex-sorting process to improve their in vitro fertilizing ability. Theriogenology 80, 350–356.
CrossRef | CAS | PubMed |

del Olmo, D., Parrilla, I., Sanchez-Osorio, J., Gomis, J., Angel, M. A., Tarantini, T., Gil, M. A., Cuello, C., Vazquez, J. L., Roca, J., Vaquez, J. M., and Martinez, E. A. (2014). Successful laparoscopic insemination with a very low number of flow cytometrically sorted boar sperm in field conditions. Theriogenology 81, 315–320.
CrossRef | PubMed |

Eriksson, B. M., Vazquez, J. M., Martinez, E. A., Roca, J., Lucas, X., and Rodriguez-Martinez, H. (2001). Effects of holding time during cooling and of type of package on plasma membrane integrity, motility and in vitro oocyte penetration ability of frozen–thawed boar spermatozoa. Theriogenology 55, 1593–1605.
CrossRef | CAS | PubMed |

Gadella, B. M., and Harrison, R. A. (2000). The capacitating agent bicarbonate induces protein kinase A-dependent changes in phospholipid transbilayer behavior in the sperm plasma membrane. Development 127, 2407–2420.
| CAS | PubMed |

Garner, D. L. (2009). Hoechst 33342: the dye that enabled differentiation of living X-and Y-chromosome bearing mammalian sperm. Theriogenology 71, 11–21.
CrossRef | CAS | PubMed |

Garner, D. L., Thomas, C. A., Gravance, C. G., Marshall, C. E., DeJarnette, J. M., and Allen, C. H. (2001). Seminal plasma addition attenuates the dilution effect in bovine sperm. Theriogenology 56, 31–40.
CrossRef | CAS | PubMed |

Garner, D. L., Evans, K. M., and Seidel, G. E. (2013). Sex-sorting sperm using flow cytometry/cell sorting. Methods Mol. Biol. 927, 279–295.
CrossRef | CAS | PubMed |

Gibb, Z., Morris, L. H., Maxwell, W. M., and Grupen, C. G. (2011). Use of a defined diluent increases the sex-sorting efficiency of stallion sperm. Theriogenology 75, 610–619.
CrossRef | CAS | PubMed |

Gosálvez, J., Ramirez, M. A., López-Fernández, C., Crespo, F., Evans, K. M., Kjelland, M. E., and Moreno, J. F. (2011). Sex-sorted bovine spermatozoa and DNA damage: II. Dynamic features. Theriogenology 75, 206–211.
CrossRef | PubMed |

Grossfeld, R., Klinc, P., Sieg, B., and Rath, D. (2005). Production of piglets with sexed semen employing a non-surgical insemination technique. Theriogenology 63, 2269–2277.
CrossRef | CAS | PubMed |

Guthrie, H. D., and Welch, G. R. (2005). Impact of storage prior to cryopreservation on plasma membrane function and fertility of boar sperm. Theriogenology 63, 396–410.
CrossRef | CAS | PubMed |

Guthrie, H. D., and Welch, G. R. (2006). Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry. J. Anim. Sci. 84, 2089–2100.
CrossRef | CAS | PubMed |

Johnson, L. A., and Welch, G. R. (1999). Sex preselection: high-speed flow cytometric sorting of X and Y sperm for maximum efficiency. Theriogenology 52, 1323–1341.
CrossRef | CAS | PubMed |

Johnson, L. A., Flook, J. P., and Hawk, H. W. (1989). Sex preselection in rabbits: live births from X- and Y-bearing sperm separated by DNA and cell sorting. Biol. Reprod. 41, 199–203.
CrossRef | CAS | PubMed |

Juarez, J. D., Parrilla, I., Vazquez, J. M., Martinez, E. A., and Roca, J. (2011). Boar semen can tolerate rapid cooling rates prior to freezing. Reprod. Fertil. Dev. 23, 681–690.
CrossRef | PubMed |

Knox, R. V. (2014). Impact of swine reproductive technologies on pig and global food production. In ‘Current and Future Reproductive Technologies and World Food Production’. (Eds G. C. Lamb and N. DiLorenzo.) pp. 131–160. (Springer: New York.)

Lee, J. A., Spidlen, J., Boyce, K., Cai, J., Crosbie, N., Dalphin, M., Furlong, J., Gasparetto, M., Goldberg, M., Goralczyk, E. M., et al. (2008). MIFlowCyt: the minimum information about a flow cytometry experiment. Cytometry A 73A, 926–930.
CrossRef |

Martinez-Alborcia, M. J., Valverde, A., Parrilla, I., Vazquez, J. M., Martinez, E. A., and Roca, J. (2012). Detrimental effects of non-functional spermatozoa on the freezability of functional spermatozoa from boar ejaculate. PLoS ONE 7, e36550.
CrossRef | CAS | PubMed |

Maxwell, W. M., and Johnson, L. A. (1997). Chlortetracycline analysis of boar spermatozoa after incubation, flow cytometric sorting, cooling, or cryopreservation. Mol. Reprod. Dev. 46, 408–418.
CrossRef | CAS | PubMed |

Okazaki, T., Abe, S., Yoshida, S., and Shimada, M. (2009). Seminal plasma damages sperm during cryopreservation, but its presence during thawing improves semen quality and conception rates in boars with poor post-thaw semen quality. Theriogenology 71, 491–498.
CrossRef | CAS | PubMed |

Parrilla, I., Vazquez, J. M., Cuello, C., Gil, M. A., Roca, J., Di Berardino, D., and Martínez, E. A. (2004). Hoechst 33342 stain and u.v. laser exposure do not induce genotoxic effects in flow-sorted boar spermatozoa. Reproduction 128, 615–621.
CrossRef | CAS | PubMed |

Parrilla, I., del Olmo, D., Sijses, L., Martinez-Alborcia, M. J., Cuello, C., Vazquez, J. M., Martinez, E. A., and Roca, J. (2012). Differences in the ability of spermatozoa from individual boar ejaculates to withstand different semen-processing techniques. Anim. Reprod. Sci. 132, 66–73.
CrossRef | PubMed |

Roca, J., Parrilla, I., Rodriguez-Martinez, H., Gil, M. A., Cuello, C., Vazquez, J. M., and Martinez, E. A. (2011). Approaches towards efficient use of boar semen in the pig industry. Reprod. Domest. Anim. 46, 79–83.
CrossRef | PubMed |

Rodríguez-Martínez, H., Saravia, F., Wallgren, M., Tienthai, P., Johannisson, A., Vázquez, J. M., Martínez, E., Roca, J., Sanz, L., and Calvete, J. J. (2005). Boar spermatozoa in the oviduct. Theriogenology 63, 514–535.
CrossRef | PubMed |

Rodríguez-Martínez, H., Saravia, F., Wallgren, M., Roca, J., and Peña, F. J. (2008). Influence of seminal plasma on the kinematics of boar spermatozoa during freezing. Theriogenology 70, 1242–1250.
CrossRef | PubMed |

Spinaci, M., Vallorani, C., Bucci, D., Bernardini, C., Tamanini, C., Seren, E., and Galeati, G. (2010). Effect of liquid storage on sorted boar spermatozoa. Theriogenology 74, 741–748.
CrossRef | CAS | PubMed |

Vallorani, C., Spinaci, M., Bucci, D., Tamanini, C., and Galeati, G. (2010). Effects of antioxidants on boar spermatozoa during sorting and storage. Anim. Reprod. Sci. 122, 58–65.
CrossRef | CAS | PubMed |

Vazquez, J. M., Martinez, E. A., Parrilla, I., Roca, J., Gil, M. A., and Vazquez, J. L. (2003). Birth of piglets after deep intrauterine insemination with flow cytometrically sorted boar spermatozoa. Theriogenology 59, 1605–1614.
CrossRef | PubMed |


   
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