CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Reproduction, Fertility and Development   
Reproduction, Fertility and Development
Journal Banner
  Vertebrate Reproductive Science & Technology
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Structure
Contacts
Content
All Issues
Special Issues
Research Fronts
Sample Issue
For Authors
General Information
Scope
Submit Article
Author Instructions
Open Access
Awards and Prizes
For Referees
Referee Guidelines
Review an Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our email Early Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter LinkedIn

red arrow Connect with SRB
blank image
facebook TwitterIcon

Affiliated Societies

RFD is the official journal of the International Embryo Transfer Society and the Society for Reproductive Biology.


 

Article << Previous     |     Next >>        Online Early    

Variation in seminal plasma alters the ability of ram spermatozoa to survive cryopreservation

J. P. Rickard A C, R. E. Schmidt A, J. W. Maddison A, R. Bathgate A, G. W. Lynch A, X. Druart B and S. P. de Graaf A

A RMC Gunn Building, Regimental Drive, Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.
B UMR 6175 INRA, CNRS-Université de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France.
C Corresponding author. Email: jessica.rickard@sydney.edu.au

Reproduction, Fertility and Development - http://dx.doi.org/10.1071/RD14123
Submitted: 10 April 2014  Accepted: 31 July 2014   Published online: 20 August 2014


 
PDF (483 KB) $25
 Supplementary Material
 Export Citation
 Print
  
Abstract

Variation in the effect of seminal plasma on sperm function and fertility has been hypothesised to be due to differences between males and their seminal plasma composition. The freezing resilience of individual rams (n = 17) was investigated to characterise inter-male variation. This was determined by measuring the degree of change in motility induced by cryopreservation (Experiment 1). Experiment 2 examined the effect of pooled seminal plasma from rams identified as having high or low resilience to freezing on the cryosurvival of washed spermatozoa from either high (n = 3) or low (n = 3) sperm freezing resilience rams. Immediately after thawing and throughout the incubation period (0–4 h), spermatozoa from high-resilience rams frozen with high-resilience seminal plasma demonstrated superior motility to spermatozoa from high-resilience rams frozen with low-resilience seminal plasma (P < 0.001). Similarly, spermatozoa from low-resilience rams frozen with high-resilience seminal plasma exhibited higher motility than spermatozoa from low-resilience rams frozen with low-resilience seminal plasma immediately after thawing (0 h; P < 0.001). The present study shows that variation in freezing resilience of ram spermatozoa is related to the source and composition of the seminal plasma.

Additional keywords: freezing resilience, semen, sheep.


References

Aller, J. F., Aguilar, D., Vera, T., Almeida, G. P., and Alberio, R. H. (2012). Seasonal variation in sexual behavior, plasma testosterone and semen characteristics of Argentine Pampinta and Corriedale rams. Spanish J. Agric. Res. 10, 345–352.
CrossRef |

Asadpour, R., Alavi-Shoushtari, S. M., Rezaii, S. A., and Ansari, M. H. K. (2007). SDS–polyacrylamide gel electrophoresis of buffalo bulls seminal plasma proteins and their relation with semen freezability. Anim. Reprod. Sci. 102, 308–313.
CrossRef | CAS | PubMed |

Aurich, J. E., Kühne, A., Hoppe, H., and Aurich, C. (1996). Seminal plasma affects membrane integrity and motility of equine spermatozoa after cryopreservation. Theriogenology 46, 791–797.
CrossRef | CAS | PubMed |

Barrios, B., Pérez-Pé, R., Gallego, M., Agustín, T., Osada, J., Muiño-Blanco, T., and Cebrián-Pérez, J. Á. (2000). Seminal plasma proteins revert the cold-shock damage on ram sperm membrane. Biol. Reprod. 63, 1531–1537.
CrossRef | CAS | PubMed |

Barrios, B., Fernandez-Juan, M., Muiño-Blanco, T., and Cebrián-Pérez, J. Á. (2005). Immunocytochemical localization and biochemical characterization of two seminal plasma proteins that protect ram spermatozoa against cold shock. J. Androl. 26, 539–549.
CrossRef | CAS | PubMed |

Bernardini, A., Hozbor, F., Sanchez, E., Fornés, M. W., Alberio, R. H., and Cesari, A. (2011). Conserved ram seminal plasma proteins bind to the sperm membrane and repair cryopreservation damage. Theriogenology 76, 436–447.
CrossRef | CAS | PubMed |

Cardozo, J. A., Fernández-Juan, M., Cebrián-Pérez, J. A., and Muiño-Blanco, T. (2008). Identification of RSVP14 and RSVP20 components by two-dimensional electrophoresis and western-blotting. Reprod. Domest. Anim. 43, 15–21.
| CAS | PubMed |

Casper, R. F., Meriano, J. S., Jarvi, K. A., Cowan, L., and Lucato, M. L. (1996). The hypo-osmotic swelling test for selection of viable sperm for intracytoplasmic sperm injection in men with complete asthenozoospermia. Fertil. Steril. 65, 972–976.
| CAS | PubMed |

de Graaf, S. P., Evans, G., Gillan, L., Guerra, M. M. P., Maxwell, W. M. C., and O’Brien, J. K. (2007a). The influence of antioxidant, cholesterol and seminal plasma on the in vitro quality of sorted and non-sorted ram spermatozoa. Theriogenology 67, 217–227.
CrossRef | CAS | PubMed |

de Graaf, S. P., Peake, K., Maxwell, W. M. C., O’Brien, J. K., and Evans, G. (2007b). Influence of supplementing diet with oleic and linoleic acid on the freezing ability and sex-sorting parameters of ram semen. Livest. Sci. 110, 166–173.
CrossRef |

Ding, Z., Qu, F., Guo, W., Ying, X., Wu, M., and Zhang, Y. (2007). Identification of sperm forward motility-related proteins in human seminal plasma. Mol. Reprod. Dev. 74, 1124–1131.
CrossRef | CAS | PubMed |

Domínguez, M. P., Falcinelli, A., Hozbor, F., Sánchez, E., Cesari, A., and Alberio, R. H. (2008). Seasonal variations in the composition of ram seminal plasma and its effect on frozen–thawed ram sperm. Theriogenology 69, 564–573.
CrossRef | PubMed |

Druart, X., Gatti, J. L., Huet, S., Dacheux, J. L., and Humblot, P. (2009). Hypotonic resistance of boar spermatozoa: sperm subpopulations and relationship with epididymal maturation and fertility. Reproduction 137, 205–213.
CrossRef | CAS | PubMed |

Druart, X., Rickard, J. P., Mactier, S., Kohnke, P. L., Kershaw-Young, C. M., Bathgate, R., Gibb, Z., Crossett, B., Tsikis, G., Labas, V., Harichaux, G., Grupen, C. G., and de Graaf, S. P. (2013). Proteomic characterization and cross species comparison of mammalian seminal plasma. J. Proteomics 91, 13–22.
CrossRef | CAS | PubMed |

Evans, G., and Maxwell, W. M. C. (1987). ‘Salamons’ Artificial Insemination of Sheep and Goats.’ (Butterworths: Sydney.)

Hernández, M., Roca, J., Calvete, J. J., Sanz, L., Muiño-Blanco, T., Cebrián-Pérez, J. A., Vázquez, J. M., and Martínez, E. (2007). Cryosurvival and in vitro fertilizing capacity postthaw is improved when boar spermatozoa are frozen in the presence of seminal plasma from good freezer boars. J. Androl. 28, 689–697.
CrossRef | PubMed |

Holt, W. V., Medrano, A., Thurston, L. M., and Watson, P. F. (2005). The significance of cooling rates and animal variability for boar sperm cryopreservation: insights from the cryomicroscope. Theriogenology 63, 370–382.
CrossRef | PubMed |

Jobim, M. I. M., Oberst, E. R., Salbego, C. G., Souza, D. O., Wald, V. B., Tramontina, F., and Mattos, R. C. (2004). Two-dimensional polyacrylamide gel electrophoresis of bovine seminal plasma proteins and their relation with semen freezability. Theriogenology 61, 255–266.
CrossRef | CAS |

Killian, G. J., Chapman, D. A., and Rogowski, L. A. (1993). Fertility-associated proteins in Holstein bull seminal plasma. Biol. Reprod. 49, 1202–1207.
CrossRef | CAS | PubMed |

Leahy, T., and de Graaf, S. P. (2012). Seminal plasma and its effect on ruminant spermatozoa during processing. Reprod. Domest. Anim. 47, 207–213.
CrossRef | PubMed |

Leahy, T., and Gadella, B. M. (2011). Sperm surface changes and physiological consequences induced by sperm handling and storage. Reproduction 142, 759–778.
CrossRef | CAS | PubMed |

Leahy, T., Evans, G., Maxwell, W. M. C., and Marti, J. I. (2010a). Seminal plasma proteins do not consistently improve fertility after cervical insemination of ewes with non-sorted or sex-sorted frozen–thawed ram spermatozoa. Reprod. Fertil. Dev. 22, 606–612.
CrossRef | CAS | PubMed |

Leahy, T., Marti, J. I., Evans, G., and Maxwell, W. M. C. (2010b). Seasonal variation in the protective effect of seminal plasma on frozen-thawed ram spermatozoa. Anim. Reprod. Sci. 119, 147–153.
CrossRef | CAS | PubMed |

Levay, P. F., le R.Fourie, F., Bezuidenhout, C., and Koch, A. (1995). Andrology: effectiveness of various sperm processing methods in removing seminal plasma from insemination media. Hum. Reprod. 10, 2056–2060.
| CAS | PubMed |

Manjunath, P., and Sairam, M. R. (1987). Purification and biochemical characterization of three major acidic proteins (BSP-A1, BSP-A2 and BSP-A3) from bovine seminal plasma. Biochem. J. 241, 685–692.
| CAS | PubMed |

Manjunath, P., Bergeron, A., Lefebvre, J., and Fan, J. (2007). Seminal plasma proteins: functions and interaction with protective agents during semen preservation. Soc. Reprod. Fertil. Suppl. 65, 217–228.
| CAS | PubMed |

Mann, T. (1964). ‘The Biochemistry of Semen.’ (Methuen and Co.: London.)

Maxwell, W. M. C., Evans, G., Mortimer, S. T., Gillan, L., Gellatly, E. S., and McPhie, C. A. (1999). Normal fertility in ewes after cervical insemination with frozen–thawed spermatozoa supplemented with seminal plasma. Reprod. Fertil. Dev. 11, 123–126.
CrossRef | CAS |

Maxwell, W. M., de Graaf, S. P., El-Hajj Ghaoui, R., and Evans, G. (2007). Seminal plasma effects on sperm handling and female fertility. Soc. Reprod. Fertil. Suppl. 64, 13–38.
| CAS | PubMed |

Mert, H., Karakus, K., Yilmaz, A., Aygun, T., Mert, N., Apaydin, B., and Seyhan, E. (2009). Effects of genotype on testis, semen quality, and mineral composition of semen in various ram breeds. Biol. Trace Elem. Res. 132, 93–102.
CrossRef | CAS | PubMed |

Muiño-Blanco, T., Pérez-Pé, R., and Cebrián-Pérez, J. Á. (2008). Seminal plasma protiens and sperm resistance to stress. Reprod. Domest. Anim. 43, 18–31.
CrossRef | PubMed |

O’Meara, C. M., Donovan, A., Hanrahan, J. P., Duffy, P., Fair, S., Evans, A. C. O., and Lonergan, P. (2007). Resuspending ram spermatozoa in seminal plasma after cryopreservation does not improve pregnancy rate in cervically inseminated ewes. Theriogenology 67, 1262–1268.
CrossRef | CAS | PubMed |

Pérez-Pé, R., Grasa, P., Fernández-Juan, M., Peleato, M. L., Cebrián-Pérez, J. Á., and Muiño-Blanco, T. (2002). Seminal plasma proteins reduce protein tyrosine phosphorylation in the plasma membrane of cold-shocked ram spermatozoa. Mol. Reprod. Dev. 61, 226–233.
CrossRef | PubMed |

Qu, F., Ying, X., Guo, W., Guo, Q., Chen, G., Liu, Y., and Ding, Z. (2007). The role of Zn-a2 glycoprotein in sperm motility is mediated by changes in cyclic AMP. Reproduction 134, 569–576.
CrossRef | CAS | PubMed |

Robertson, S. A. (2007). Seminal fluid signaling in the female reproductive tract: lessons from rodents and pigs. J. Anim. Sci. 85, E36–E44.
CrossRef | CAS | PubMed |

Roca, J., Hernández, M., Carvajal, G., Vázquez, J. M., and Martínez, E. A. (2006). Factors influencing boar sperm cryosurvival. J. Anim. Sci. 84, 2692–2699.
CrossRef | CAS | PubMed |

Souza, C. E. A., Rego, J. P. A., Lobo, C. H., Oliveira, J. T. A., Nogueira, F. C. S., Domont, G. B., Fioramonte, M., Gozzo, F. C., Moreno, F. B., Monteiro-Moreira, A. C. O., Figueiredo, J. R., and Moura, A. A. (2012). Proteomic analysis of the reproductive tract fluids from tropically-adapted Santa Ines rams. J. Proteomics 75, 4436–4456.
CrossRef | CAS |

Swamy, M. J. (2004). Interaction of bovine seminal plasma proteins with model membranes and sperm plasma membranes. Curr. Sci. 87, 203–211.
| CAS |

Talevi, R., and Gualtieri, R. (2010). Molecules involved in sperm-oviduct adhesion and release. Theriogenology 73, 796–801.
CrossRef | CAS | PubMed |

Thurston, L. M., Watson, P. F., Mileham, A. J., and Holt, W. V. (2001). Morphologically distinct sperm subpopulations defined by Fourier shape descriptors in fresh ejaculates correlate with variation in boar semen quality following cryopreservation. J. Androl. 22, 382–394.
| CAS | PubMed |

Troedsson, M. H., Desvousges, A., Alghamdi, A. S., Dahms, B., Dow, C. A., Hayna, J., Valesco, R., Collahan, P. T., Macpherson, M. L., Pozor, M., and Buhi, W. C. (2005). Components in seminal plasma regulating sperm transport and elimination. Anim. Reprod. Sci. 89, 171–186.
CrossRef | CAS | PubMed |

Yu, I., Songsasen, N., Godke, R. A., and Leibo, S. P. (2002). Differences among dogs in response of their spermatozoa to cryopreservation using various cooling and warming rates. Cryobiology 44, 62–78.
CrossRef | CAS | PubMed |

Zahn, F. S., Papa, F. O., Melo, C. M., and Brisola, M. L. (2005). Protein profile of equine seminal plasma: correlation to semen freezability. Anim. Reprod. Sci. 89, 313–315.
| CAS | PubMed |


   
Subscriber Login
Username:
Password:  

 
    
Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2015