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RESEARCH ARTICLE

Gelatinases and their tissue inhibitors are associated with oxidative stress: a potential set of markers connected with male infertility

Ewa M. Kratz A E , Anna Kałuża A , Mirosława Ferens-Sieczkowska A , Beata Olejnik A , Renata Fiutek B , Mariusz Zimmer C and Agnieszka Piwowar D
+ Author Affiliations
- Author Affiliations

A Department of Chemistry and Immunochemistry, Wrocław Medical University, O. Bujwida 44A, 50-345 Wrocław, Poland.

B Department of Laboratory Diagnostics, Wrocław Medical University, Academic Hospital, Borowska 213, 50-556 Wrocław, Poland.

C 2nd Department and Clinic of Gynaecology, Obstetrics and Neonatology, Wrocław Medical University, Academic Hospital, Borowska 213, 50-556 Wrocław, Poland.

D Department of Toxicology, Wrocław Medical University, Borowska 211, 50-556 Wrocław, Poland.

E Corresponding author. Email: ewa.kratz@umed.wroc.pl

Reproduction, Fertility and Development 28(7) 1029-1037 https://doi.org/10.1071/RD14268
Submitted: 22 March 2014  Accepted: 16 November 2014   Published: 7 January 2015

Abstract

The expression and activity of matrix metalloproteinases (MMPs) may be regulated by oxidative stress in various pathophysiological processes; therefore, the aim of the present study was to analyse the associations between the expression of the gelatinases MMP-9 and MMP-2 and their tissue inhibitors TIMP-1, TIMP-2 and levels of total antioxidant capacity (TAC) and advanced oxidation protein products (AOPP) in seminal plasma prepared for artificial insemination. Levels of MMPs and TIMPs were evaluated using ELISA, whereas TAC and AOPP in the seminal plasma of 131 childless men and 38 fertile volunteers were determined spectrophotometrically. Seminal MMP-9 expression was higher in childless men than in fertile subjects, whereas there was no significant differences in MMP-2 expression between the analysed seminal groups. TIMP-1 and TIMP-2 expression was similar in all groups. However, TAC expression was significantly higher in infertile normozoospermic and oligozoospermic men and AOPP expression was higher in astheno-, oligo- and normozoospermic infertile patients than in fertile men. High AOPP, together with an increased MMP-9 : TIMP-1 ratio alters the oxidative–antioxidative balance of the ejaculate, thereby reducing male fertility, and therefore these parameters may serve as additional diagnostic markers of semen quality and male reproductive potential.

Additional keywords: human seminal plasma.


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