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Article << Previous     |         Contents Vol 52(1)

Effects of addition of nitrogen on soil fungal and bacterial biomass and carbon utilisation efficiency in a city lawn soil

Xinyu Jiang A C , Lixiang Cao B and Renduo Zhang A C

A Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China.
B School of Life Science, Sun Yat-sen University, Guangzhou 510275, China.
C Corresponding authors. Email: zhangrd@mail.sysu.edu.cn; XIN.YU.JIANG1@gmail.com

Soil Research 52(1) 97-105 http://dx.doi.org/10.1071/SR13210
Submitted: 2 May 2013  Accepted: 23 September 2013   Published: 5 February 2014


 
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Abstract

The aim of this study was to investigate the effects of nitrogen (N) addition on soil microbial (fungal and bacterial) biomass and carbon utilisation efficiency (CUE) in a city lawn soil. A field experiment was conducted with three N treatments (kg N ha–1 year–1): the control (0), low-N (100), and high-N (200). Soil biogeochemical properties including pH, C : N, CUE, microbial biomass C (MBC), fungal and bacterial biomass, microbial C uptake rates, and soil respiration (SR) rates were determined during a 500-day experiment. The low- and high-N treatments significantly decreased soil pH, MBC, and CUE. Available N and soil acidification caused a decline in soil MBC. Soil acidification was not beneficial for microbial biomass growth, especially for bacteria. The treatments with N changed soil biomass from bacterial-dominant to fungal-dominant.

The results also showed that the CUE of bacterial-dominant soil was higher than that of fungal-dominant soil, which is contrary to previous studies. However, SR did not increase with decreased CUE under N treatments, because the addition of N limited soil microbial C uptake rates and significantly decreased soil microbial biomass. The CUE showed a negative correlation with soil temperature for the control treatment but not for the N treatments, which suggested that added N played a more important role in CUE than did soil temperature. Our results showed that addition of further N significantly alters soil biogeochemical properties, alters the ratio of bacteria to fungi, and decreases microbial carbon utilisation, which should provide important information for model-based prediction of soil C-cycling.

Additional keywords: carbon utilisation efficiency, microbial biomass, N treatment, soil acidification, soil carbon, soil respiration.


References

Aciego Pietri JC, Brookes PC (2009) Substrate inputs and pH as factors controlling microbial biomass, activity and community structure in an arable soil. Soil Biology & Biochemistry 41, 1396–1405.
CrossRef | CAS |

Allison SD, Wallenstein MD, Bradford MA (2010) Soil-carbon response to warming dependent on microbial physiology. Nature Geoscience 3, 336–340.
CrossRef | CAS |

Bardgett RD, McAlister E (1999) The measurement of soil fungal: bacterial biomass ratios as an indicator of ecosystem self-regulation in temperate meadow grasslands. Biology and Fertility of Soils 29, 282–290.
CrossRef |

Bardgett RD, Lovell RD, Hobbs PJ, Jarvis SC (1999) Seasonal changes in soil microbial communities along a fertility gradient of temperate grasslands. Soil Biology & Biochemistry 31, 1021–1030.
CrossRef | CAS |

Bell JM, Smith JL, Bailey VL, Bolton H (2003) Priming effect and C storage in semi-arid no-till spring crop rotations. Biology and Fertility of Soils 37, 237–244.

Bi J, Zhang NL, Liang Y, Yang HJ, Ma KP (2012) Interactive effects of water and nitrogen addition on soil microbial communities in a semiarid steppe. Journal of Plant Ecology 5, 320–329.
CrossRef |

Boberg J, Finlay RD, Stenlid J, Nasholm T, Lindahl BD (2008) Glucose and ammonium additions affect needle decomposition and carbon allocation by the litter degrading fungus Mycena epipterygia. Soil Biology & Biochemistry 40, 995–999.
CrossRef | CAS |

Bossuyt H, Denef K, Six J, Frey SD, Merckx R, Paustian K (2001) Influence of microbial populations and residue quality on aggregate stability. Applied Soil Ecology 16, 195–208.
CrossRef |

Bottomley PJ (1994) Light microscopic methods for studying soil microorganisms. In ‘Methods of soil analysis. Part 2. Microbiological and biochemical properties’. (Ed. RW Weaver) pp. 81–105. (Soil Science Society of America: Madison, WI)

Bowden RD, Davidson E, Savage K, Arabia C, Steudler P (2004) Chronic nitrogen additions reduce total soil respiration and microbial respiration in temperate forest soils at the Harvard Forest. Forest Ecology and Management 196, 43–56.
CrossRef |

Boyle D (1998) Nutritional factors limiting the growth of Lentinula edodes and other white-rot fungi in wood. Soil Biology & Biochemistry 30, 817–823.
CrossRef | CAS |

Brink RH, Dubach P, Lynch DL (1960) Measurement of carbohydrate in soil hydrolyzates with anthrone. Soil Science 89, 157–166.
CrossRef | CAS |

Busse MD, Sanchez FG, Ratcliff AW, Butnor JR, Carter EA, Powers RE (2009) Soil carbon sequestration and changes in fungal and bacterial biomass following incorporation of forest residues. Soil Biology & Biochemistry 41, 220–227.
CrossRef | CAS |

Compton JE, Watrud LS, Porteous LA, DeGrood S (2004) Response of soil microbial biomass and community composition to chronic nitrogen additions at Harvard forest. Forest Ecology and Management 196, 143–158.
CrossRef |

Dalal RC, Mayer RJ (1986) Long term trends in fertility of soils under continuous cultivation and cereal cropping in southern Queensland. II. Total organic carbon and its rate of loss from the soil profile. Soil Research 24, 281–292.
CrossRef | CAS |

Devêvre OC, Horwáth WR (2000) Decomposition of rice straw and microbial carbon use efficiency under different soil temperatures and moistures. Soil Biology & Biochemistry 32, 1773–1785.
CrossRef |

Fang Y, Xun F, Bai WM, Zhang WH, Li LH (2012) Long-term nitrogen addition leads to loss of species richness due to litter accumulation and soil acidification in a temperate steppe. PLoS ONE.
CrossRef | PubMed |

Follett RF, Paul EA, Pruessner EG (2007) Soil carbon dynamics during a long-term incubation study involving 13C and 14C measurements. Soil Science 172, 189–208.
CrossRef | CAS |

Frey SD, Gupta VVSR, Elliott ET, Paustian K (2001) Protozoan grazing affects estimates of carbon utilization efficiency of the soil microbial community. Soil Biology & Biochemistry 33, 1759–1768.
CrossRef | CAS |

Groffman PM, Pouyat RV (2009) Methane uptake in urban forests and lawns. Environmental Science & Technology 43, 5229–5235.
CrossRef | CAS |

Groffman PM, Williams CO, Pouyat RV, Band LE, Yesilonis ID (2009) Nitrate leaching and nitrous oxide flux in urban forests and grasslands. Journal of Environmental Quality 38, 1848–1860.
CrossRef | CAS | PubMed |

Hamer U, Potthast K, Makeschin F (2009) Urea fertilisation affected soil organic matter dynamics and microbial community structure in pasture soils of Southern Ecuador. Applied Soil Ecology 43, 226–233.
CrossRef |

Hobbie SE (2000) Interactions between lignin and soil nitrogen availability during leaf litter decomposition in a Hawaiian montane forest. Ecosystems 3, 484–494.
CrossRef | CAS |

Hobbie SE, Gough L (2004) Litter decomposition in moist acidic and non-acidic tundra with different glacial histories. Oecologia 140, 113–124.
CrossRef | PubMed |

Hobbie SE, Nadelhoffer KJ, Högberg P (2002) A synthesis: the role of nutrients as constraints on carbon balances in boreal and arctic regions. Plant and Soil 242, 163–170.
CrossRef | CAS |

Ingham ER, Klein DA (1984) Soil fungi: relationships between hyphal activity and staining with fluorescein diacetate. Soil Biology & Biochemistry 16, 273–278.
CrossRef | CAS |

Jim CY, Chen WY (2006) Perception and attitude of residents toward urban green spaces in Guangzhou (China). Environmental Management 38, 338–349.
CrossRef | CAS | PubMed |

Johnson D, Leake JR, Lee JA, Campbell CD (1998) Changes in soil microbial biomass and microbial activities in response to 7 years simulated pollutant nitrogen deposition on a heathland and two grasslands. Environmental Pollution 103, 239–250.
CrossRef | CAS |

Katharina MK, Edward KH, Wolfgang W, Ute S, Ieda H, Gunther E, Sandra B, Joseph S, Katja S, Andreas R, Sophie ZB (2010) The effect of resource quantity and resource stoichiometry on microbial carbon-use-efficiency. FEMS Microbiology Ecology 73, 430–440.

Keiblinger KM, Hall EK, Wanek W, Szukics U, Hämmerle I, Ellersdorfer G, Böck S, Strauss J, Sterflinger K, Richter A, Zechmeister-Boltenstern S (2010) The effect of resource quantity and resource stoichiometry on microbial carbon-use efficiency. FEMS Microbiology Ecology 73, 430–440.

Krusche AV, De Camargo PB, Cerri CE, Ballester MV, Lara LBLS, Victoria RL, Martinelli LA (2003) Acid rain and nitrogen deposition in a sub-tropical watershed (Piracicaba): ecosystem consequences. Environmental Pollution 121, 389–399.
CrossRef | CAS | PubMed |

Li LJ, Zeng DH, Yu ZY, Fan ZP, Mao R (2010) Soil microbial properties under N and P additions in a semi-arid, sandy grassland. Biology and Fertility of Soils 46, 653–658.
CrossRef | CAS |

Lin Z, Zhang R, Tang J, Zhang J (2011) Effects of high soil water content and temperature on soil respiration. Soil Science 176, 150–155.
CrossRef | CAS |

López-Urrutia A, Morán XAG (2007) Resource limitation of bacterial production distorts the temperature dependence of oceanic carbon cycling. Ecology 88, 817–822.
CrossRef | PubMed |

Lu XK, Mo JM, Gundersern P, Zhu WX, Zhou GY, Li DJ, Zhang X (2009) Effect of simulated N deposition on soil exchangeable cations in three forest types of subtropical China. Pedosphere 19, 189–198.
CrossRef | CAS |

Magid J, Kjaergaard GA, Kuikman PJ (1999) Drying and rewetting of a loamy sand soil did not increase turnover of native organic matter, but retarded the decomposition of added 14C-labelled plant material. Soil Biology & Biochemistry 31, 595–602.
CrossRef | CAS |

Magill AH, Aber JD, Hendricks JJ, Bowden RD, Melillo JM, Steudler PA (1997) Biogeochemical response of forest ecosystems to simulated chronic nitrogen deposition. Ecological Applications 7, 402–415.
CrossRef |

Micks P, Aber JD, Boone RD, Davidson EA (2004) Short-term soil respiration and nitrogen immobilization response to nitrogen applications in control and nitrogen-enriched temperate forests. Forest Ecology and Management 196, 57–70.
CrossRef |

Mo JM, Zhang W, Zhu WX, Fang YT, Li DJ, Zhao P (2007) Response of soil respiration to simulated N deposition in a disturbed and a rehabilitated tropical forest in southern China. Plant and Soil 296, 125–135.
CrossRef | CAS |

Nilsson LO, Wallander H, Gundersen P (2012) Changes in microbial activities and biomasses over a forest floor gradient in C-to-N ratio. Plant and Soil 355, 75–86.
CrossRef | CAS |

Parton WJ, Schimel DS, Cole CV, Ojima DS (1987) Analysis of factors controlling soil organic matter levels in Great Plains grasslands. Soil Science Society of America Journal 51, 1173–1179.
CrossRef | CAS |

Rousk J, Bååth E (2007) Fungal and bacterial growth in soil with plant materials of different C/N ratios. FEMS Microbiology Ecology 62, 258–267.
CrossRef | CAS | PubMed |

Rousk J, Demoling LA, Bahr A, Bååth E (2008) Examining the fungal and bacterial niche overlap using selective inhibitors in soil. FEMS Microbiology Ecology 63, 350–358.
CrossRef | CAS | PubMed |

Rousk J, Brookes PC, Bååth E (2009) Contrasting soil pH effects on fungal and bacterial growth suggest functional redundancy in carbon mineralization. Applied and Environmental Microbiology 75, 1589–1596.
CrossRef | CAS | PubMed |

Schimel JP, Gulledge JM, Clein-Curley JS, Lindstrom JE, Braddock JF (1999) Moisture effects on microbial activity and community structure in decomposing birch litter in the Alaskan taiga. Soil Biology & Biochemistry 31, 831–838.
CrossRef | CAS |

Söderström BE (1977) Vital staining of fungi in pure cultures and in soil with fluorescein diacetate. Soil Biology & Biochemistry 9, 59–63.
CrossRef |

Steinweg JM, Plante AF, Conant RT, Paula EA, Tanaka DL (2008) Patterns of substrate utilization during long-term incubations at different temperatures. Soil Biology & Biochemistry 40, 2722–2728.
CrossRef | CAS |

Teklay T, Nordgren A, Nyberg G, Malmer A (2007) Carbon mineralization of leaves from four Ethiopian agroforestry species under laboratory and field conditions. Applied Soil Ecology 35, 193–202.
CrossRef |

Thiet RK, Frey SD, Six J (2006) Do growth yield efficiencies differ between soil microbial communities differing in fungal: bacterial ratios? Reality check and methodological issues. Soil Biology & Biochemistry 38, 837–844.
CrossRef | CAS |

Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring microbial biomass C. Soil Biology & Biochemistry 19, 703–707.
CrossRef | CAS |

Xu RK, Ji GL (2001) Effects of H2SO4 and HNO3 on soil acidification and aluminum speciation in variable and constant charge soils. Water, Air, and Soil Pollution 129, 33–43.
CrossRef | CAS |

Yeomans JC, Bremner JM (1988) A rapid and precise method for routine determination of organic carbon in soil. Communications in Soil Science and Plant Analysis 19, 1467–1476.
CrossRef | CAS |

Zak DR, Ringelberg DB, Pregitzer KS, Randlett DL, White DC, Curtis PS (1996) Soil microbial communities beneath Populus grandidentata grown under elevated atmospheric CO2. Ecological Applications 6, 257–262.
CrossRef |

Zhang NL, Wan SQ, Li LH, Bi J, Zhao MM, Ma KP (2008) Impacts of urea N addition on soil microbial community in a semi-arid temperate steppe in northern China. Plant and Soil 311, 19–28.
CrossRef | CAS |

Zhao X, Xing G (2009) Variation in the relationship between nitrification and acidification of subtropical soils as affected by the addition of urea or ammonium sulfate. Soil Biology & Biochemistry 41, 2584–2587.
CrossRef | CAS |


   
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