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RESEARCH ARTICLE

Effects of intercropping grasses on soil organic carbon and microbial community functional diversity under Chinese hickory (Carya cathayensis Sarg.) stands

Jiasen Wu A B , Haiping Lin A C , Cifu Meng B , Penkun Jiang B and Weijun Fu B C
+ Author Affiliations
- Author Affiliations

A The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Lin′an 311300, China.

B Zhejiang Provincial Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration, Zhejiang A & F University, Lin′an 311300, China.

C Corresponding authors. Emails: fuweijun@zafu.edu.cn; 13396557805@163.com

Soil Research 52(6) 575-583 https://doi.org/10.1071/SR14021
Submitted: 24 January 2014  Accepted: 15 May 2014   Published: 19 August 2014

Abstract

Chinese hickory (Carya cathayensis Sarg.) is a woody nut and oil tree from China. Intensive management including heavy application of chemical fertiliser and long-term application of herbicides has resulted in serious soil loss and degradation. This study aimed to test the hypothesis that intercropping in the soil under Chinese hickory stands may improve soil fertility and microbial community functional diversity. A field experiment consisting of four treatments (clean tillage; intercropping rape (Brassica rapa L.), ryegrass (Lolium perenne L.) or Chinese milk vetch (Astragalus sinicus L.) was conducted to study the effects of intercropping on soil organic carbon (SOC) structure and microbial community functional diversity under C. cathayensis stand, by means of 13C-nuclear magnetic resonance (NMR), and EcoPlates incubated at 25°C.

After 4 years of treatment, intercropping increased available nitrogen (N), phosphorus and potassium in the soil by 25.1–54.2, 4.2–6.0 and 0–22.5 mg kg–1, respectively, relative to the clean tillage treatment; intercropping rape, ryegrass and Chinese milk vetch increased SOC, microbial biomass C (MBC), and water-soluble organic C (WOC) by 23.1–24.7, 138.6–159.7 and 56.2–69.5% (P < 0.05), respectively. The structure of SOC was also greatly changed by intercropping treatments. Intercropping increased carbonyl C by 29.9–36.9% (P < 0.05) and decreased alkyl C, O-alkyl C and aromatic C by 10.0–16.4, 18.9–20.9 and 10.5–16.6% (P < 0.05), respectively. Intercropping markedly improved microbial community functional diversity, which is characterised by increases in average well-colour development (AWCD), Shannon index and evenness index. Correlation analysis showed significant positive correlations among microbial biomass N, water-soluble organic N, SOC, WOC, MBC and AWCD (P < 0.05 or P < 0.01). The results demonstrate that sod cultivation is an effective soil management practice that improves soil quality and eliminates detrimental effects of clean tillage in Chinese hickory production.

Additional keywords: cover crop, interplanting, microbial functional diversity, 13C-nuclear magnetic resonance.


References

Abou-Zeid MA (2012) Pathogenic variation in isolates of Pseudomonas causing the brown blotch of cultivated mushroom, Agaricus bisporus. Brazilian Journal of Microbiology 43, 1137–1146.
Pathogenic variation in isolates of Pseudomonas causing the brown blotch of cultivated mushroom, Agaricus bisporus.Crossref | GoogleScholarGoogle Scholar | 24031938PubMed |

Agricultural Chemistry Committee of China (1983) ‘Conventional methods of soil and agricultural chemistry analysis.’ (Science Press: Beijing) [in Chinese]

An SS, Li GH, Chen LD (2011) Soil microbial functional diversity between rhizosphere and non-rhizosphere of typical plants in the hilly area of southern Nixia. Acta Ecologica Sinica 31, 5225–5234. [in Chinese]

Beyer L (1995) Soil microbial biomass and organic-matter composition in soils under cultivation. Biology and Fertility of Soils 19, 197–202.
Soil microbial biomass and organic-matter composition in soils under cultivation.Crossref | GoogleScholarGoogle Scholar |

Bossio DA, Girvan MS, Verchot L, Bullimore J, Borelli T, Albrecht A, Scow KM, Ball AS, Pretty JN, Osborn AM (2005) Soil microbial community response to land use change in an agricultural landscape of western Kenya. Microbial Ecology 49, 50–62.
Soil microbial community response to land use change in an agricultural landscape of western Kenya.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXjslyis70%3D&md5=27120c211d7ce37c3dbb74752474976bCAS | 15690227PubMed |

Bünemann EK, Bossio DA, Smithson PC, Frossard E, Oberson A (2004) Microbial community composition and substrate use in a highly weathered soil as affected by crop rotation and P fertilization. Soil Biology & Biochemistry 36, 889–901.
Microbial community composition and substrate use in a highly weathered soil as affected by crop rotation and P fertilization.Crossref | GoogleScholarGoogle Scholar |

Cairney JWG (2000) Evolution of mycorrhiza systems. Naturwissenschaften 87, 467–475.
Evolution of mycorrhiza systems.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXosVSisr0%3D&md5=dd5e5a26739a00f49f95cafde12024f5CAS |

Chen CR, Xu ZH, Mathers NJ (2004) Soil carbon pools in adjacent natural and plantation forests of subtropical Australia. Soil Science Society of America Journal 68, 282–291.
Soil carbon pools in adjacent natural and plantation forests of subtropical Australia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXmsFarsw%3D%3D&md5=e3e5eb71372b89021b23e555aee5a05dCAS |

Chen SQ, Huang JQ, Huang XZ, Lou Z, Lu JQ, Xia GH, Wu JS (2010) Nutrient elements in soil and Carya cathayensis leaves from four parent rock materials. Journal of Zhejiang Forestry College 27, 572–578. [in Chinese]

Clapp CE, Hayes MHB (1999) Size and shapes of humic substances. Soil Science 164, 777–789.
Size and shapes of humic substances.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXnvVahu7w%3D&md5=0c731f963f0682a0e8a3c5b9ac29b17bCAS |

Clenn DM, Welket W (1991) Control tree productivity through root systems: orchard floor systems of the future. Compact Fruit Tree 24, 45–46.

Degens BP, Schipper LA, Sparling GP, Vojvodic–Vukovic M (2000) Decreases in organic C reserves in soils can reduce the catabolic diversity of soil microbial communities. Soil Biology & Biochemistry 32, 189–196.
Decreases in organic C reserves in soils can reduce the catabolic diversity of soil microbial communities.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXhsVyhsrw%3D&md5=cf3bed2821a307ef4fc04c263c96d4b1CAS |

Doran JW, Parkin TB (1994) Defining and assessing soil quality. In ‘Defining soil quality for a sustainable environment’. Soil Science Society of America Publication No. 35. (Ed. JW Doran) pp. 3–21. (SSSA: Madison, WI, USA)

FAO (2006) ‘Global forest resource assessment 2005.’ (Food and Agricultural Organization of the United Nations: Rome)

Giller PS (1996) The diversity of soil communities, the poor man’s tropical forest. Biodiversity and Conservation 5, 135–168.
The diversity of soil communities, the poor man’s tropical forest.Crossref | GoogleScholarGoogle Scholar |

Giller KE, Beare MH, Lavelle P, Izac AMN, Swift MJ (1997) Agricultural intensification, soil biodiversity and agroecosystem function. Applied Soil Ecology 6, 3–16.
Agricultural intensification, soil biodiversity and agroecosystem function.Crossref | GoogleScholarGoogle Scholar |

Goulding KWT, Murphy DV, Macdonald A, Stockdale EA, Gaunt JL, Blake L, Ayaga G, Brookes P (2000) The role of soil organic matter and manures in sustainable nutrient cycling. In ‘Sustainable management of soil organic matter’. (Eds RM Rees, BC Ball, CD Campbell, CA Watson) pp. 221–232. (CABI: Wallingford, UK)

Hofman J, Švihálek J, Holoubek I (2004) Evaluation of functional diversity of soil microbial communities—a case study. Plant, Soil and Environment 50, 141–148.

Huang ZQ, Xu ZH, Chen CR, Boyd S (2008) Changes in soil carbon during the establishment of a hardwood plantation in subtropical Australia. Forest Ecology Management 254, 46–55.
Changes in soil carbon during the establishment of a hardwood plantation in subtropical Australia.Crossref | GoogleScholarGoogle Scholar |

Huang ZQ, Clinton PW, Davis MR (2011) Impacts of plantation forest management on soil organic matter quality. Journal of Soils and Sediments 11, 1309–1316.
Impacts of plantation forest management on soil organic matter quality.Crossref | GoogleScholarGoogle Scholar |

Jandl R, Lindner M, Vesterdal L, Bauwens B, Baritz R, Hagedorn F, Johnson DW, Minkkinen K, Byrne KA (2007) How strongly can forest management influence soil carbon sequestration. Geoderma 137, 253–268.
How strongly can forest management influence soil carbon sequestration.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXnvFCgsA%3D%3D&md5=fb252a327f45ad73cd9cb575aeddda76CAS |

Jiang PK, Yu YW, Jin AW, Wang AG, Yu QM (2000) Analysis on soil nutrients of soil under high-yield Phyllostachys praecox f. prevelnalis forest. Journal of Bamboo Research 19, 50–53. [in Chinese]

Jiang PK, Xu QF, Zhou GM (2009) ‘Soil quality under Phyllostachys praecox stands and its evolution trend.’ pp. 263–267. (Agriculture Press: Beijing) [in Chinese]

Jing AW (1999) A preliminary study on degenerative mechanism of Phyllostachys praecox stand planted in a protected site. Journal of Fujing Forestry College 19, 94–96. [in Chinese]

Jones DL, Willett VB (2006) Experimental evaluation of methods to quantify dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) in soil. Soil Biology & Biochemistry 38, 991–999.
Experimental evaluation of methods to quantify dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) in soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XjvFSitr4%3D&md5=7183443f037caf12106b9849cf597ea3CAS |

Kanchikerimath M, Singh D (2001) Soil organic matter and biological properties after 26 years of maize–wheat–cowpea cropping as affected by manure and fertilization in a Cambisol in semiarid region of India. Agriculture, Ecosystems & Environment 86, 155–162.
Soil organic matter and biological properties after 26 years of maize–wheat–cowpea cropping as affected by manure and fertilization in a Cambisol in semiarid region of India.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXksVynu7Y%3D&md5=2f0e59da080eadd7b32c07770e8aa520CAS |

Kennedy AC, Gewin VL (1997) Soil microbial diversity: present and future considerations. Soil Science 162, 607–617.
Soil microbial diversity: present and future considerations.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXmsV2ksbY%3D&md5=103dee69f66755c4cda5893302ede480CAS |

Klironomos JN, McCune J, Hart M, Neville J (2000) The influence of arbuscular mycorrhizae on the relationship between plant diversity and productivity. Ecology Letters 3, 137–141.
The influence of arbuscular mycorrhizae on the relationship between plant diversity and productivity.Crossref | GoogleScholarGoogle Scholar |

Larson WE, Pierce FJ (1991) Conservation and enhancement of soil quality. In ‘Evaluation for sustainable land management in the developing world. Proceedings of the International Workshop on Evaluation for Sustainable Land Management in the Developing World’. Bangkok, Thailand. (International Board for Soil Research and Management)

Li HK (2008) Eco-environmental effect and integrated technical system of green cover in apple orchard in Weibei Dryland Farming Areas. PhD Thesis, Northwest A & F University, Shanxi, China.

Li YF, Jiang PK, Chang SX, Wu JS, Lin L (2010) Organic mulch and fertilization affect soil carbon pools and forms under intensively managed bamboo (Phyllostachys praecox) forests in southeast China. Journal of Soils and Sediments 10, 739–747.
Organic mulch and fertilization affect soil carbon pools and forms under intensively managed bamboo (Phyllostachys praecox) forests in southeast China.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXntlKksr0%3D&md5=b420a5225f76f4c2ef32c9e0a26fd530CAS |

Lupwayi NZ, Rice WA, Clayton GW (1998) Soil microbial diversity and community structure under wheat as influenced by tillage and crop rotation. Soil Biology & Biochemistry 30, 1733–1741.
Soil microbial diversity and community structure under wheat as influenced by tillage and crop rotation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXlt1Smtbo%3D&md5=06eb85eb85f90b7b049531fd52811730CAS |

Mancinelli R, Campiglia E, Tizio AD, Marinari S (2010) Soil carbon dioxide emission and carbon content as affected by conventional and organic cropping systems in Mediterranean environment. Applied Soil Ecology 46, 64–72.
Soil carbon dioxide emission and carbon content as affected by conventional and organic cropping systems in Mediterranean environment.Crossref | GoogleScholarGoogle Scholar |

Meng P, Zhang JS (2003) Effects of silvo pastoral system ecology and economics in the hilly land of Taihang Mountain. Chinese Journal of Eco-Agriculture 11, 12–15. [in Chinese]

Pan ZS, Wang QL, Lu Y (2004) Research on Increasing effect about combining fruit grass with stock raising in grape orchard. Journal of Anhui Agricultural Sciences 32, 492–493. [in Chinese]

Qian XY, Zheng HJ, Zhao WM (2010) Study on screening the excellent green manures under Carya cathayensis forest. East China Forest Management 24, 24–25. [in Chinese]

Spaccini R, Mbagwu JSC, Conte P, Piccolo A (2006) Changes of humic substances characteristics from forested to cultivated soils in Ethiopia. Geoderma 132, 9–19.
Changes of humic substances characteristics from forested to cultivated soils in Ethiopia.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xjt1Kgs7k%3D&md5=37a7d0fd4044241598bc3ed0a2e4249bCAS |

Stark A, Bushati N, Jan CH, Kheradpour P (2008) A single Hox locus in Drosophila produces functional micro-RNAs from opposite DNA strands. Genes & Development 22, 8–13.
A single Hox locus in Drosophila produces functional micro-RNAs from opposite DNA strands.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXks1Sguw%3D%3D&md5=1c2a2e3477fa0a898715c0e1fdd55a2cCAS |

Tatzber M, Stemmer M, Spiegel H (2008) Impact of different tillage practices on molecular characteristics of humic acids in a long-term field experiment—an application of three different spectroscopic methods. The Science of the Total Environment 406, 256–268.
Impact of different tillage practices on molecular characteristics of humic acids in a long-term field experiment—an application of three different spectroscopic methods.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXht1Shs7fM&md5=8d78c543fc466727e67d0abed1a1c687CAS | 18789814PubMed |

Uchida Y, Nishimura S, Akiyama H (2012) The relationship of water-soluble carbon and hot-water-soluble carbon with soil respiration in agricultural fields. Agriculture, Ecosystems & Environment 156, 116–122.
The relationship of water-soluble carbon and hot-water-soluble carbon with soil respiration in agricultural fields.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XovVylurY%3D&md5=644f8ebbb6adaa7f58aafdb40807cbd5CAS |

Ussiri DAN, Johnson CE (2003) Characterization organic matter in a northern hardwood forest soil by 13C NMR spectroscopy and chemical methods. Geoderma 111, 123–149.
Characterization organic matter in a northern hardwood forest soil by 13C NMR spectroscopy and chemical methods.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xpslaitr8%3D&md5=ae22f5d51199d61261eaf5a07f639f9dCAS |

Vance ED, Brookes PC, Jenkinson DC (1987) An extraction method for measuring soil microbial biomass C. Soil Biology & Biochemistry 19, 703–707.
An extraction method for measuring soil microbial biomass C.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1cXjs1KqsA%3D%3D&md5=619d5036dacd709b54824b460cae8c88CAS |

Wang ZJ, Huang XZ, Tang XH, Huang JQ, Qian LF, Li ZJ (2011) Analysis on economic and ecological benefits of no-tillage management of Carya cathayensis. Acta Ecologica Sinica 21, 1285–1289. [in Chinese]

Webster EA, Hopkins DW, Chudek JA, Haslam SFI, Šimek M, Pîcek T (2001) The relationship between microbial carbon and the resource quality of soil carbon. Journal of Environmental Quality 30, 147–150.
The relationship between microbial carbon and the resource quality of soil carbon.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXhvFSlsb0%3D&md5=3abefd0e9147df6af1e665f307fdbcd6CAS | 11215646PubMed |

Xia W, Yan JM, Zhu AG (2007) Soil erosion prevention in pecan forest land. Journal of Zhejiang Water Conservancy & Hydropower College 4, 70–72. [in Chinese]

Xu MX (1998) ‘Cultivation technology of fruit trees in arid areas.’ pp. 167–171. (Jindun Press: Beijing)[in Chinese]

Xu HJ, Xiao RL, Song TQ, Luo W, Li SH (2007) Effects of long-term fertilization on functional diversity of soil microbial community of the tea plantation. Acta Ecologica Sinica 27, 3355–3361. [in Chinese]

Xu QF, Jiang PK, Wang QZ, Lu YT (2009) Effects of green manure on soil microbial properties of Phyllostachys pubescens stands under intensive management. Journal of Beijing Forestry University 31, 43–48. [in Chinese]

Yan XJ, Huang JQ, Qiu ZM, Nuramina RHM, Zhu MH, Wu JS (2012) Soil physical and chemical properties and fruit quality with grass cover in a Myrica rubra orchard. Journal of Zhejiang A&F University 28, 850–854.

Yang YH, Hua XM (2000) Effect of pesticide pollution against functional microbial diversity in soil. Journal of Microbiology 20, 23–47. [in Chinese]

Yao H, Bowman D, Shi W (2006) Soil microbial community structure and diversity in a turfgrass chronosequence: Land–use change versus turfgrass management. Applied Soil Ecology 34, 209–218.
Soil microbial community structure and diversity in a turfgrass chronosequence: Land–use change versus turfgrass management.Crossref | GoogleScholarGoogle Scholar | [in Chinese]

Yu L, Chen J, Chen LJ (2011) Effect of interplantation of green manure varieties on yield of hickory forests. China Forestry Science and Technology 25, 92–95. [in Chinese]

Zhang HH, Tang M, Chen H, Du XG (2008) Diversity of soil microbial communities in the mycorrhizosphere of five afforestation tree species in the Loess Plateau. Journal of Beijing Forestry University 30, 85–90. [in Chinese]