
%0 Journal Article
%A Huo, Li-Jun 
%A Yue, Kui-Zhong 
%A Yang, Zeng-Ming 
%T Characterization of viability, mitochondrial activity, acrosomal integrity and capacitation status in boar sperm during <emph type="2">in vitro</emph> storage at different ambient temperatures
%J Reproduction, Fertility and Development
%D 2003/01/29
%V 14
%N 8
%P 509-514
%K boar, capacitation, mitochondrion, semen, viability.
%R https://doi.org/10.1071/RD02035
%U https://www.publish.csiro.au/paper/RD02035 
%X Extended storage of unfrozen boar semen becomes an alternative because the use of frozen&#8211;thawed boar sperm results in low fertility. Sperm viability, mitochondrial activity, capacitation and acrosome integrity of freshly ejaculated boar semen stored in vitro for up to 48 h at 4&#176;C, 15&#176;C, 20&#176;C and 39&#176;C was characterized during the study. The viability of boar sperm was assessed by both Hoechst 33258 and SYBR-14/PI staining. Mitochondrial function was assessed by JC-1 staining. Capacitation status was determined by chlortetracycline (CTC)/Hoechst 33258 staining. The acrosome integrity was analysed with Coomassie blue staining. These data were derived from three ejaculates each from three crossbred boars. The viabilities assessed with SYBR-14/PI, Hoechst 33258 and JC-1 staining correlated highly (r&#160;&#62;&#160;0.980). In freshly ejaculated boar semen, 96 &#177; 1% of the sperm did not take up the Hoechst 33258, whereas 95 &#177; 2% were stained by SYBR-14 and 96 &#177; 2% of the sperm had mitochondria exhibiting positive JC-1 staining. Staining with CTC/Hoechst 33258 suggested that a high percentage of sperm became capacitated after 24 h storage at 15&#176;C and 20&#176;C. There were 62 &#177; 2% (15&#176;C) and 89 &#177; 2% (20&#176;C) capacitated sperm by 48 h. Moreover, most of the capacitated sperm were acrosome intact. These results suggest that SYBR-14/PI, Hoechst 33258 or JC-1 staining can be used to effectively evaluate the quality of boar sperm during in vitro storage. 
