
%0 Journal Article
%A Monet-Kuntz, C 
%A Fontaine, I 
%T Secretion of transferrin in ovine seminiferous tubule cell cultures in response to FSH: influence of breed, season of birth and age of lambs
%J Reproduction, Fertility and Development
%D 1993/02/01
%V 5
%N 1
%P 83-93
%K 
%R https://doi.org/10.1071/RD9930083
%U https://www.publish.csiro.au/paper/RD9930083 
%X The response of lamb Sertoli cells to follicle stimulating hormone (FSH) was investigated by measuring transferrin secretion in seminiferous tubule cell cultures throughout the non-pubertal and the prepubertal periods. Cells could be cultured from birth until they attained a testicular weight of 19 g. The characteristics of individual dose-response curves were compared according to the breed, season of birth and testicular weight of the lambs. At the same season of birth and within a given testis weight range, dose-response curves of Romanov and Ile-de-France lambs were similar. Within a given testis weight range, spring-born animals exhibited a higher maximal transferrin secretion than autumn-born lambs, but the ED50 was similar. The main factor of variation of the dose-response curve parameters was the testicular weight of the lambs: the amplitude of FSH response increased 3-fold from a testicular weight of 6 g onwards, i.e. from the appearance of spermatogonia in seminiferous tubules. The ED50 increased 5-fold from 11 g onwards, i.e. from the beginning of the prepubertal period. Thus, Sertoli cells become less sensitive to FSH as spermatogenesis develops in seminiferous tubules. This phenomenon is largely the result of higher phosphodiesterase activity and is greatly reduced by 1-methyl-3-isobutyl-xanthine (MIX). 
