Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Sperm-mediated gene transfer

Marialuisa Lavitrano A B , Marco Busnelli A , Maria Grazia Cerrito A , Roberto Giovannoni A , Stefano Manzini A and Alessia Vargiolu A
+ Author Affiliations
- Author Affiliations

A Dipartimento di Scienze Chirurgiche e Terapia Intensiva, Università di Milano, Bicocca, 20052 Milano, Italy.

B Corresponding author. Email: marialuisa.lavitrano@unimib.it

Reproduction, Fertility and Development 18(2) 19-23 https://doi.org/10.1071/RD05124
Submitted: 21 September 2005  Accepted: 21 September 2005   Published: 14 December 2005

Abstract

Since 1989, a new method for the production of transgenic animals has been available, namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to bind and internalise exogenous DNA molecules and to transfer them into the oocyte at fertilisation. We first described the SMGT procedure in a small animal model, with high efficiency reported in the mouse. In addition, we successfully adapted and optimised the technique for use in large animals; it was, in fact, highly efficient in the generation of human decay accelerating factor transgenic pig lines, as well as multigene transgenic pigs in which three different reporter genes, namely enhanced green fluorescent protein, enhanced blue fluorescent protein and red fluorescent protein, were introduced. The major benefits of the SMGT technique were found to be its high efficiency, low cost and ease of use compared with other methods. Furthermore, SMGT does not require embryo handling or expensive equipment. Sperm-mediated gene transfer could also be used to generate multigene transgenic pigs that would be of benefit as large animal models for medical research, for agricultural and pharmaceutical applications and, in particular, for xenotransplantation, which requires extensive genetic manipulation of donor pigs to make them suitable for grafting to humans.

Extra keywords: sperm cells, transgenic animals.


Acknowledgments

The authors’ work reported herein was funded by the Italian Ministry of Agriculture (DM 581/7240/96, DM 564/7240/97, DM 404/7240/99, DM 36/7303/02) and by the Italian Minister of Research and University (DD 21.09.99, n462 ric). The authors thank Elena Giovannoni for assistance and the care of animals.


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