Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology

Induction of the ovulatory LH surge in Asian elephants (Elephas maximus): a novel aid in captive breeding management of an endangered species

Chatchote Thitaram A B F , Pornsawan Pongsopawijit A , Saran Chansitthiwet C , Janine L. Brown D , Kannikar Nimtragul E , Khajhonpat Boonprasert C , Pongpon Homkong C , Sittidet Mahasawangkul C , Suvichai Rojanasthien A , Ben Colenbrander B , Gysbert C. van der Weijden B and Frank J. C. M. van Eerdenburg B
+ Author Affliations
- Author Affliations

A Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.

B Faculty of Veterinary Medicine, Utrecht University, Marburglaan 2, 3584 CN, Utrecht, The Netherlands.

C Thai Elephant Conservation Center, National Elephant Institute, Forest Industry Organisation, Lampang, 52190, Thailand.

D Conservation and Research Center, National Zoological Park, Smithsonian Institution, Front Royal,Virginia 22630, USA.

E Chiang Mai Zoo, The Zoological Park Organisation Under the Royal Patronage of H. M. The King, Chiang Mai, 50200, Thailand.

F Corresponding author. Email:

Reproduction, Fertility and Development 21(5) 672-678
Submitted: 17 December 2008  Accepted: 16 March 2009   Published: 22 May 2009


A unique feature of the reproductive physiology of Asian elephants (Elephas maximus) is the occurrence of two LH surges before ovulation, instead of one. An anovulatory LH (anLH) surge, the function of which is unknown, occurs consistently 3 weeks before the ovulatory LH (ovLH) surge that induces ovulation. Thus, the ability to induce an ovLH surge would be useful for scheduling natural mating or artificial insemination. The present study tested the efficacy of a gonadotrophin-releasing hormone agonist (GnRH-Ag) to induce LH surges during the follicular phase of the oestrous cycle, which resulted in varied LH responses, but generally none were as high as previously documented natural surges. Thus, for the ovulation-induction trials, nine females were administered 80 μg GnRH-Ag intravenously at three time periods during the oestrous cycle, namely the anovulatory follicular phase, the ovulatory follicular phase and the luteal phase. During the late anovulatory follicular phase, nine of 10 females (90%) responded with an immediate LH surge followed 15–22 days later by an ovLH surge or a post-ovulatory increase in progestagens. In contrast, despite responding to the GnRH-Ag with an immediate increase in LH, none of the females treated during other periods of the oestrous cycle exhibited subsequent ovLH surges. One cow got pregnant from natural mating following the induced ovLH surge. In conclusion, ovLH induction is possible using a GnRH-Ag, but only during a specific time of the anovulatory follicular phase.

Additional keywords: gonadotropin-releasing hormone, ovulation induction.


The authors thank Intervet/Schering-Plough Animal Health (Thailand) Ltd, the National Research Council of Thailand, Royal Thai Government scholarship, Thailand, and the Smithsonian Women’s Committee, USA, for research funding. The authors also thank Mr Pichai Jirawattanapong, Utrecht University, for statistical assistance, Ms Ratchaneewan Khejornwong and Ms Kannikar Timhom, Chiang Mai Zoo, for endocrine laboratory assistance, Dr A. F. Parlow, NIDDK National Hormone and Pituitary Program, USA, for endocrine laboratory support, Professor Tom Stout, Utrecht University, and Dr Thomas Hildebrandt, Institute of Zoo Biology and Wildlife Research, Berlin, for scientific assistance, and Mr Worawit Rojanaphaitoon, National Elephant Institute, for allowing the study of the elephants. Finally, the authors thank all the mahouts and staff of the Thai Elephant Conservation Center.


Brown, J. L. (2000). Reproductive endocrine monitoring of elephants: an essential tool for assisting captive management. Zoo Biol. 19, 347–367.
CrossRef | CAS |

Brown, J. L. , and Lehnhardt, J. (1997). Secretory patterns of serum prolactin in Asian (Elephas maximus) and African (Loxodonta africana) elephants during different reproductive states: comparison with concentrations in a noncycling African elephant. Zoo Biol. 16, 149–159.
CrossRef | CAS |

Brown, J. , Hildebrandt, T. , Theison, W. , and Neiffer, D. (1999a). Endocrine and ultrasound evaluation of a non-cycling African elephant: identification of an ovarian follicular cyst. Zoo Biol. 18, 223–232.
CrossRef | CAS |

Brown, J. L. , Schmitt, D. L. , Bellem, A. , Graham, L. H. , and Lehnhardt, J. (1999b). Hormone secretion in the Asian elephant (Elephas maximus): characterization of ovulatory and anovulatory luteinizing hormone surges. Biol. Reprod. 61, 1294–1299.
CrossRef | PubMed | CAS |

Brown, J. L. , Goritz, F. , Pratt-Hawkes, N. , Hermes, R. , and Galloway, M. , et al. (2004). Successful artificial insemination of an Asian elephant at the National Zoological Park. Zoo Biol. 23, 45–63.
CrossRef |

Chappel, S. C. , and Schmidt, M. (1979). Cyclic release of luteinizing hormone and the effects of luteinizing hormone-releasing hormone injection in Asiatic elephants. Am. J. Vet. Res. 40, 451–453.
PubMed | |  CAS |

Czekala, N. M. , MacDonald, E. A. , Steinman, K. , Walker, S. , Garrigues, N. W. , and Olson, D. (2003). Estrogen and LH dynamics during the follicular phase of the estrous cycle in the Asian elephant. Zoo Biol. 22, 443–454.
CrossRef | CAS |

De Rensis, F. , and Peters, A. (1999). The control of follicular dynamics by PGF2α, GnRH, hCG and oestrus synchronization in cattle. Reprod. Domest. Anim. 34, 49–59.
CrossRef | CAS |

Graham, L. H. , Bolling, J. , Miller, G. , Pratt-Hawkes, N. , and Joseph, S. (2002). Enzyme-immunoassay for the measurement of luteinizing hormone in the serum of African elephants (Loxodonta africana). Zoo Biol. 21, 403–408.
CrossRef | CAS |

Heistermann, M. , Trohorsch, B. , and Hodges, J. K. (1997). Assessment of ovarian function in the African elephant (Loxodonta africana) by measurement of 5α-reduced progesterone metabolites in serum and urine. Zoo Biol. 16, 273–284.
CrossRef | CAS |

Hermes, R. , Olson, D. , Göritz, F. , Brown, J. L. , Schmitt, D. L. , Hagan, D. , Peterson, J. S. , Fritsch, G. , and Hildebrandt, T. B. (2000). Ultrasonography of the estrous cycle in female African elephants (Loxodonta africana). Zoo Biol. 19, 369–382.
CrossRef |

Hermes, R. , Goritz, F. , Streich, W. , and Hildebrandt, T. (2007). Assisted reproduction in female rhinoceros and elephants: current status and future perspective. Reprod. Domest. Anim. 42(Suppl.), 33–44.
CrossRef | PubMed |

Hildebrandt, T. B. , Goeritz, F. , Hermes, R. , Reid, C. , Dehnhard, M. , and Brown, J. L. (2006). Aspects of the reproductive biology and breeding management of Asian and African elephants: Elephas maximus and Loxodonta africana. Int. Zoo Yb. 40, 20–40.
CrossRef |

Hodges, J. K. (1998). Endocrinology of the ovarian cycle and pregnancy in the Asian (Elephas maximus) and African (Loxodonta africana) elephant. Anim. Reprod. Sci. 53, 3–18.
CrossRef | PubMed | CAS |

Jagger, J. P. , Peters, A. R. , and Lamming, G. E. (1987). Hormone responses to low-dose GnRH treatment in post-partum beef cows. J. Reprod. Fertil. 80, 263–269.
CrossRef | PubMed | CAS |

Leong, K. M. , Ortolani, A. , Graham, L. H. , and Savage, A. (2003). The use of low-frequency vocalizations in African elephant (Loxodonta africana) reproductive strategies. Horm. Behav. 43, 433–443.
CrossRef | PubMed | CAS |

Munro, C. , and Stabenfeldt, G. (1984). Development of a microtitre plate enzyme immunoassay for the determination of progesterone. J. Endocrinol. 101, 41–49.
CrossRef | PubMed | CAS |

Ortolani, A. , Leong, K. , Graham, L. , and Savage, A. (2005). Behavioral indices of estrus in a group of captive African elephants (Loxodonta africana). Zoo Biol. 24, 311–329.
CrossRef | CAS |

Peters, A. R. (2005). Veterinary clinical application of GnRH: questions of efficacy. Anim. Reprod. Sci. 88, 155–167.
CrossRef | PubMed | CAS |

Ptaszynska M. (2006). ‘Compendium of Animal Reproduction.’ (Intervet International: Boxmeer, The Netherlands.)

Purves, R. D. (1992). Optimum numerical integration methods for estimation of area-under-the-curve (AUC) and area-under-the-moment-curve (AUMC). J. Pharmacokinet. Biopharm. 20, 211–226.
CrossRef | PubMed | CAS |

Schulte, B. A. , Feldman, E. , Lambert, R. , Oliver, R. , and Oliver, R. (2000). Temporary ovarian inactivity in elephants: relationship to status and time outside. Physiol. Behav. 71, 123–131.
CrossRef | PubMed | CAS |

Thitaram, C. , Brown, J. L. , Pongsopawijit, P. , Chansitthiwet, S. , and Wongkalasin, W. , et al. (2008). Seasonal effects on the endocrine pattern of semi-captive female Asian elephants (Elephas maximus): timing of the anovulatory luteinizing hormone surge determines the length of the estrous cycle. Theriogenology 69, 237–244.
CrossRef | PubMed | CAS |

Yeh, K. C. , and Kwan, K. C. (1978). A comparison of numerical integrating algorithms by trapezoidal, Lagrange, and spline approximation. J. Pharmacokinet. Biopharm. 6, 79–98.
CrossRef | PubMed | CAS |

Youngquist R. S., and Threlfall W. R. (2007). ‘Current Therapy in Large Animal Theriogenology.’ (Saunders Elsevier: St Louis.)

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