Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Transgenerational inheritance of ovarian development deficiency induced by maternal diethylhexyl phthalate exposure

Xi-Feng Zhang A B , Teng Zhang A , Zhe Han B , Jing-Cai Liu A C , Yu-Ping Liu D , Jun-Yu Ma A , Lan Li A and Wei Shen A E
+ Author Affiliations
- Author Affiliations

A Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.

B College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China.

C College of Life Science, Qingdao Agricultural University, Qingdao 266109, China.

D Hospital of School, Qingdao Agricultural University, Qingdao 266109, China.

E Corresponding author. Email: shenwei427@163.com

Reproduction, Fertility and Development 27(8) 1213-1221 https://doi.org/10.1071/RD14113
Submitted: 26 March 2014  Accepted: 5 May 2014   Published: 12 June 2014

Abstract

Diethylhexyl phthalate (DEHP) is a widely used industrial additive for increasing plastic flexibility. It disrupts the physiological functions of endogenous hormones and induces abnormal development of mammals. The objectives of the present study were to evaluate the effects of DEHP exposure on ovarian development of pregnant mice and whether the effects are inheritable. We found that the synthesis of oestradiol in pregnant mice after DEHP exposure was significantly decreased, and that the first meiotic progression of female fetal germ cells was delayed. Furthermore, the DNA methylation level of Stra8 was increased and the expression levels of Stra8 were significantly decreased. An accelerated rate of follicle recruitment in F1 mice was responsible for the depletion of the primordial-follicle pool. Maternal DEHP exposure also significantly accelerated the recruitment of primordial follicles in F2 mice. In conclusion, our results indicated that maternal DEHP exposure induced ovarian development deficiency, which was transgenerational in mice.

Additional keywords: DEHP, follicle, meiosis, oocyte, premature oocyte failure.


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