Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

An integrated approach to bovine oocyte quality: from phenotype to genes

Leslie Angulo A B C D , Catherine Guyader-Joly E , Sylvain Auclair A B C , Christelle Hennequet-Antier F , Pascal Papillier A B C , Mekki Boussaha G , Sébastien Fritz D G , Karine Hugot G , François Moreews H I , Claire Ponsart D J , Patrice Humblot D K and Rozenn Dalbies-Tran A B C L
+ Author Affiliations
- Author Affiliations

A INRA, UMR85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France.

B CNRS, UMR7247, F-37380 Nouzilly, France.

C Université François Rabelais de Tours, F-37041 Tours, France.

D Union Nationale des Coopératives d’Elevage et d’Insémination Animale, F-94704 Maisons-Alfort, France.

E Union Nationale des Coopératives d’Elevage et d’Insémination Animale, F-38300 Chateauvillain, France.

F INRA, UR83 Recherches Avicoles, F-37380 Nouzilly, France.

G INRA, UMR 1313 Génétique Animale et Biologie Intégrative, 78350 Jouy-en-Josas, France.

H INRA Sigenae, UMR1348 PEGASE 35042 Rennes, France.

I Genscale, Institut de recherche en informatique et systèmes aléatoires (IRISA), 35042 Rennes, France.

J ANSES, Laboratoire de Santé Animale, 14 rue Pierre et Marie Curie, F-94701 Maisons-Alfort, France.

K Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.

L Corresponding author. Email: dalbies@tours.inra.fr

Reproduction, Fertility and Development 28(9) 1276-1287 https://doi.org/10.1071/RD14353
Submitted: 20 September 2014  Accepted: 7 January 2015   Published: 18 February 2015

Abstract

In cattle, early embryonic failure plays a major role in the limitation of reproductive performance and is influenced by genetic effects. Suboptimal oocyte quality, including an inadequate store of maternal factors, is suspected to contribute to this phenomenon. In the present study, 13 Montbeliarde cows were phenotyped on oocyte quality, based on their ability to produce viable embryos after in vitro maturation, fertilisation and culture for 7 days. This discriminated two groups of animals, exhibiting developmental rates below 18.8% or above 40.9% (relative to cleaved embryos). Using microarrays, transcriptomic profiles were compared between oocytes collected in vivo from these two groups of animals. The difference in oocyte development potential was associated with changes in transcripts from 60 genes in immature oocytes and 135 genes in mature oocytes (following Bonferroni 5% correction). Of these, 16 and 32 genes were located in previously identified fertility quantitative trait loci. A subset of differential genes was investigated on distinct samples by reverse transcription–quantitative polymerase chain reaction. For SLC25A16, PPP1R14C, ROBO1, AMDHD1 and MEAF6 transcripts, differential expression was confirmed between high and low oocyte potential animals. Further sequencing and searches for polymorphisms will pave the way for implementing their use in genomic selection.

Additional keywords: development, embryo, fertility, gene expression, transcriptome.


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