Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Cytoskeletal alterations associated with donor age and culture interval for equine oocytes and potential zygotes that failed to cleave after intracytoplasmic sperm injection

Elena Ruggeri A , Keith F. DeLuca B , Cesare Galli C D , Giovanna Lazzari D , Jennifer G. DeLuca B and Elaine M. Carnevale A E
+ Author Affiliations
- Author Affiliations

A Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 1601 Campus Delivery, Fort Collins, CO 80523, USA.

B Department of Biochemistry and Molecular Biology, College of Natural Sciences, Colorado State University, 1870 Campus Delivery, Fort Collins, CO 80523, USA.

C Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di sopra, 50, 40064, Ozzano Emilia (Bologna), Italy.

D Avantea srl, Laboratory of Reproductive Technologies, Via Porcellasco 7f, 26100 Cremona, Italy.

E Corresponding author. Email: elaine.carnevale@colostate.edu

Reproduction, Fertility and Development 27(6) 944-956 https://doi.org/10.1071/RD14468
Submitted: 25 November 2014  Accepted: 18 February 2015   Published: 24 March 2015

Abstract

Intracytoplasmic sperm injection (ICSI) is an established method to fertilise equine oocytes, but not all oocytes cleave after ICSI. The aims of the present study were to examine cytoskeleton patterns in oocytes after aging in vitro for 0, 24 or 48 h (Experiment 1) and in potential zygotes that failed to cleave after ICSI of oocytes from donors of different ages (Experiment 2). Cytoplasmic multiasters were observed after oocyte aging for 48 h (P < 0.01). A similar increase in multiasters was observed with an increased interval after ICSI for young mares (9–13 years) but not old (20–25 years) mares. Actin vesicles were observed more frequently in sperm-injected oocytes from old than young mares. In the present study, multiasters appeared to be associated with cell aging, whereas actin vesicles were associated with aging of the oocyte donor.

Additional keywords: actin, maternal aging, oocyte senescence, tubulin.


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