Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Breeding in the fat-tailed dunnart following ovarian suppression with the gonadotrophin-releasing hormone agonist Lucrin® Depot

Ryan R. Witt A B C , John J. Rodger A B and John C. Rodger A B
+ Author Affiliations
- Author Affiliations

A FAUNA Research Alliance, School of Environmental and Life Sciences, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia.

B Science Building, University of Newcastle, University Drive, Callaghan, NSW 2308, Australia.

C Corresponding author. Email: ryan.witt@newcastle.edu.au

Reproduction, Fertility and Development - https://doi.org/10.1071/RD16518
Submitted: 22 December 2016  Accepted: 24 July 2017   Published online: 10 August 2017

Abstract

Lucrin Depot (AbbVie), a 1-month microsphere gonadotrophin-releasing hormone (GnRH) agonist preparation, was investigated as a potential agent to synchronise cycling in the fat-tailed dunnart (Sminthopsis crassicaudata). Forty-eight randomly selected females were treated with 5 or 10 mg kg-1 Lucrin Depot (n = 24 per dose). Eighteen females per treatment had their reproductive activity scored at 4, 8, 12 and 16 weeks using two ovarian (Graafian follicle and corpus luteum status) and two reproductive tract (uterine and vaginal muscularity and vascularity) parameters that formed a reproductive activity score. Six females per treatment were paired with a male at 4 weeks. Fertility was assessed between 8 and 16 weeks by pouch check, and thereafter by dissection. The effects of the 5 and 10 mg kg-1 doses were statistically equivalent. Females showed suppression at 4–8 weeks, an increase in reproductive activity at 8–12 weeks and all were cycling normally at 16 weeks. Six pouch young were born at 12 weeks to two females treated with the 5 mg kg-1 dose. Nine embryos were recovered at 16 weeks from two females treated with the 10 mg kg-1 dose. In conclusion, Lucrin Depot can suppress breeding, and fertile mating can occur in subsequent cycles in the dunnart. There is potential for Lucrin Depot to be used as an assisted breeding tool, but it may need to be combined with ovarian stimulation treatment to achieve practical levels of synchronisation in the fat-tailed dunnart.

Additional keywords: assisted reproduction, dasyurid, female synchronisation, marsupial.


References

Aleem, F. A., and Predanic, M. (1995). The hemodynamic effect of GnRH agonist therapy on uterine leioyoma vascularity: a prospective study using transvaginal color Doppler sonography. Gynecol. Endocrinol. 9, 253–258.
The hemodynamic effect of GnRH agonist therapy on uterine leioyoma vascularity: a prospective study using transvaginal color Doppler sonography.CrossRef | 1:STN:280:DyaK287ivFentg%3D%3D&md5=52868592e315bbb6a239f2a97fe1db58CAS |

Amiridis, G. S., Belibasaki, S., Leontides, L., Lymberopoulos, A., and Vainas, E. (2000). Reproductive efficiency of three estrus synchronization schemes comprising fixed-time insemination in dairy cows. J. Vet. Med. A Physiol. Pathol. Clin. Med. 47, 271–276.
Reproductive efficiency of three estrus synchronization schemes comprising fixed-time insemination in dairy cows.CrossRef | 1:STN:280:DC%2BD3M%2FhtVGntA%3D%3D&md5=dadc4f48c5c73efba57e6c7548b214b4CAS |

Asa, C. S. (1993). The development of contraceptive methods for captive wildlife. In ‘USDA National Wildlife Research Center Symposia: Contraception in Wildlife Management.’ (Ed. T. J. Kreeger.) pp. 235–240. (Denver, Colorado)

Au, P. C. K., Nation, A., Parrott, M., and Selwood, L. (2010). Induced ovulation mimics the time-table of natural development in the stripe-faced dunnart, Sminthopsis macroura and results in the birth of fertile young. Reproduction 139, 419–425.
Induced ovulation mimics the time-table of natural development in the stripe-faced dunnart, Sminthopsis macroura and results in the birth of fertile young.CrossRef | 1:CAS:528:DC%2BC3cXitFKitLs%3D&md5=8543e9ff44154faaf69ba127c9cf74baCAS |

Ballantyne, K., Anderson, S. T., Pyne, M., Nicolson, V., Mucci, A., Lisle, A. T., and Johnston, S. D. (2016a). The use of a synthetic progesterone, levonorgestrel (LNG), to control the oestrous cycle in the koala. Reprod. Fertil. Dev. 28, 1526–1532.
The use of a synthetic progesterone, levonorgestrel (LNG), to control the oestrous cycle in the koala.CrossRef | 1:CAS:528:DC%2BC28Xhtl2msbfL&md5=f101185cad08961aebe4731eae9fe2a0CAS |

Ballantyne, K., Anderson, S. T., Pyne, M., Nicolson, V., Mucci, A., Lisle, A. T., and Johnston, S. D. (2016b). Use of the gonadotrophin-releasing hormone antagonist azaline B to control the oestrous cycle in the koala (Phascolarctos cinereus). Reprod. Fertil. Dev. 28, 1686–1694.
Use of the gonadotrophin-releasing hormone antagonist azaline B to control the oestrous cycle in the koala (Phascolarctos cinereus).CrossRef | 1:CAS:528:DC%2BC28XhsFSlsb3F&md5=fdc74e9d86cf0aa37a2e2e26e8891ae2CAS |

Bedford, J. M., and Breed, W. G. (1994). Regulated storage and subsequent transformation of spermatozoa in the fallopian tubes of an Australian marsupial, Sminthopsis crassicaudata. Biol. Reprod. 50, 845–854.
Regulated storage and subsequent transformation of spermatozoa in the fallopian tubes of an Australian marsupial, Sminthopsis crassicaudata.CrossRef | 1:STN:280:DyaK2c3lslOmug%3D%3D&md5=d72212c77bb183a7a0b7f4a01e6dc01dCAS |

Bennett, J. H., Breed, W. G., Hayman, D. L., and Hope, R. M. (1989). Reproductive and genetical studies with a laboratory colony of the dasyurid marsupial Sminthopsis crassicaudata. Aust. J. Zool. 37, 207–222.
Reproductive and genetical studies with a laboratory colony of the dasyurid marsupial Sminthopsis crassicaudata.CrossRef |

Borman, J. M., Radcliff, R. P., McCormack, B. L., Kojima, F. N., Patterson, D. J., Macmillan, K. L., and Lucy, M. C. (2003). Synchronisation of oestrus in dairy cows using prostaglandin F, gonadotrophin-releasing hormone, and oestradiol cypionate. Anim. Reprod. Sci. 76, 163–176.
Synchronisation of oestrus in dairy cows using prostaglandin F, gonadotrophin-releasing hormone, and oestradiol cypionate.CrossRef | 1:CAS:528:DC%2BD3sXhtVCgt7Y%3D&md5=80c677905e5389a0edd883f4730f2b88CAS |

Breed, W. G., Leigh, C. M., and Bennett, J. H. (1989). Sperm morphology and storage in the female reproductive tract of the fat-tailed dunnart, Sminthopsis crassicaudata (Marsupialia: Dasyuridae). Gamete Res. 23, 61–75.
Sperm morphology and storage in the female reproductive tract of the fat-tailed dunnart, Sminthopsis crassicaudata (Marsupialia: Dasyuridae).CrossRef | 1:STN:280:DyaL1MzgslWiuw%3D%3D&md5=7e80098b49a1110b4592b766efa1d6c4CAS |

Conn, P. M., and Crowley, W. F. (1994). Gonadotropin-releasing hormone and its analogs. Annu. Rev. Med. 45, 391–405.
Gonadotropin-releasing hormone and its analogs.CrossRef | 1:CAS:528:DyaK2cXis1Wnsrc%3D&md5=474d226bfa5f53ece8593b65843b402eCAS |

Crawford, J. L., McLeod, B. J., and Hurst, P. R. (1999). Cyclical changes in epithelial cells of the vaginal cul-de-sac of brushtail possums (Trichosurus vulpecula). Anat. Rec. 254, 307–321.
Cyclical changes in epithelial cells of the vaginal cul-de-sac of brushtail possums (Trichosurus vulpecula).CrossRef | 1:STN:280:DyaK1M7psVehtA%3D%3D&md5=8f17fa5ca185a05bc49895ad4b88716fCAS |

Crawford, J. L., McLeod, B. J., and Eckery, D. C. (2011). The hypothalamic–pituitary–ovarian axis and manipulations of the oestrous cycle in the brushtail possum. Gen. Comp. Endocrinol. 170, 424–448.
The hypothalamic–pituitary–ovarian axis and manipulations of the oestrous cycle in the brushtail possum.CrossRef | 1:CAS:528:DC%2BC3MXntVamtQ%3D%3D&md5=8d4a04a4d6a4305e26b6b25e94785656CAS |

Czarny, N. A., Garnham, J. I., Harris, M. S., and Rodger, J. C. (2009). Comparison of the production, quality, and in vitro maturation capacity of oocytes from untreated cycling and intermediate phase equine serum gonadotropin-treated fat-tailed dunnarts (Sminthopsis crassicaudata). Reproduction 138, 23–31.
Comparison of the production, quality, and in vitro maturation capacity of oocytes from untreated cycling and intermediate phase equine serum gonadotropin-treated fat-tailed dunnarts (Sminthopsis crassicaudata).CrossRef | 1:CAS:528:DC%2BD1MXovFemsLc%3D&md5=65e9390ef1f56d34e93ad92674b0b40eCAS |

Evans, A. C. O., Duffy, P., Crosby, T. F., Hawken, P. A. R., Boland, M. P., and Beard, A. P. (2004). Effect of ram exposure at the end of progestagen treatment on estrus synchronisation and fertility during the breeding season in ewes. Anim. Reprod. Sci. 84, 349–358.
Effect of ram exposure at the end of progestagen treatment on estrus synchronisation and fertility during the breeding season in ewes.CrossRef | 1:CAS:528:DC%2BD2cXmsVCqu7c%3D&md5=e867b3ef801078e38fd67895303a54f6CAS |

Eymann, J., Herbert, C. A., Thomson, B. P., Trigg, T. E., Cooper, D. W., and Eckery, D. C. (2007). Effects of deslorelin implants on reproduction in the common brushtail possum (Trichosurus vulpecula). Reprod. Fertil. Dev. 19, 899–909.
Effects of deslorelin implants on reproduction in the common brushtail possum (Trichosurus vulpecula).CrossRef | 1:CAS:528:DC%2BD2sXht1eqsrzL&md5=d365ea13617b516a52c116d0b6211112CAS |

Eymann, J., Cooper, D. W., and Herbert, C. A. (2013). Brushtail possums (Trichosurus vulpecula) in metropolitan Sydney: population biology and response to Suprelorin contraceptive implants. Aust. J. Zool. 61, 78–86.
Brushtail possums (Trichosurus vulpecula) in metropolitan Sydney: population biology and response to Suprelorin contraceptive implants.CrossRef |

Fierro, S., Gil, J., Vinoles, C., and Olivera-Muzante, J. (2013). The use of prostaglandins in controlling estrous cycle of the ewe: a review. Theriogenology 79, 399–408.
The use of prostaglandins in controlling estrous cycle of the ewe: a review.CrossRef | 1:CAS:528:DC%2BC38XhvVSltrzN&md5=33f5a77c35b62b99b638cfb8b5376b59CAS |

Fierro, S., Vinoles, C., and Olivera-Muzante, J. (2016). Concentrations of steroid hormones, estrous, ovarian andreproductive responses in sheep estrous synchronized with different prostaglandin-based protocols. Anim. Reprod. Sci. 167, 74–82.
Concentrations of steroid hormones, estrous, ovarian andreproductive responses in sheep estrous synchronized with different prostaglandin-based protocols.CrossRef | 1:CAS:528:DC%2BC28XivVKnu74%3D&md5=32b736b58bad0adc0c84bdb5dee7b2baCAS |

Fricke, P. M., Guenther, J. N., and Wiltbank, M. C. (1998). Efficacy of decreasing the dose of GnRH used in a protocol for synchronisation of ovulation and timed AI in lactating dairy cows. Theriogenology 50, 1275–1284.
Efficacy of decreasing the dose of GnRH used in a protocol for synchronisation of ovulation and timed AI in lactating dairy cows.CrossRef | 1:CAS:528:DyaK1MXktlCksA%3D%3D&md5=6be4901c137c9248a6016ee5174accd6CAS |

Frigo, L., and Woolley, P. A. (1997). Growth and development of pouch young of the stripe-faced dunnart, Sminthopsis macroura (Marsupialia: Dasyuridae), in captivity. Aust. J. Zool. 45, 157–170.
Growth and development of pouch young of the stripe-faced dunnart, Sminthopsis macroura (Marsupialia: Dasyuridae), in captivity.CrossRef |

Gemmell, R. T. (1995). A comparative study of the corpus luteum. Reprod. Fertil. Dev. 7, 303–312.
A comparative study of the corpus luteum.CrossRef | 1:STN:280:DyaK287oslClsA%3D%3D&md5=28011908e97c55fe09966410ebc0fb23CAS |

Herbert, C. A., and Trigg, T. E. (2005). Applications of GnRH in the control and management of fertility in female animals. Anim. Reprod. Sci. 88, 141–153.
Applications of GnRH in the control and management of fertility in female animals.CrossRef | 1:CAS:528:DC%2BD2MXpslygtro%3D&md5=7d4e32c01649845838e3a4ef0c53626eCAS |

Herbert, C. A., Trigg, T. E., and Cooper, D. W. (2004). Effect of deslorelin implants on follicular development parturition and post-partum oestrus in the tammar wallaby (Macropus eugenii). Reproduction 127, 265–273.
Effect of deslorelin implants on follicular development parturition and post-partum oestrus in the tammar wallaby (Macropus eugenii).CrossRef | 1:CAS:528:DC%2BD2cXitVWrtL0%3D&md5=db0e851c447887ef1ae2939c511c8c41CAS |

Herbert, C. A., Trigg, T. E., Renfree, M. B., Shaw, G., Eckery, D. C., and Cooper, D. W. (2005). Long-term effects of deslorelin implants on reproduction in the female tammar wallaby (Macropus eugenii). Reproduction 129, 361–369.
Long-term effects of deslorelin implants on reproduction in the female tammar wallaby (Macropus eugenii).CrossRef | 1:CAS:528:DC%2BD2MXjslSntLY%3D&md5=f356a56bf423ebcb428f5e053165de37CAS |

Hesterman, H., Jones, S. M., and Schwarzenberger, F. (2008a). Reproductive endocrinology of the largest dasyurids: characterisation of ovarian cycles by plasma and fecal steriod monitoring. Part I. The Tasmanian devil (Sarcophilius harrasii). Gen. Comp. Endocrinol. 155, 234–244.
Reproductive endocrinology of the largest dasyurids: characterisation of ovarian cycles by plasma and fecal steriod monitoring. Part I. The Tasmanian devil (Sarcophilius harrasii).CrossRef | 1:CAS:528:DC%2BD2sXhtlGiu77M&md5=de154ce6490f3effeecd1acd979d484cCAS |

Hesterman, H., Jones, S. M., and Schwarzenberger, F. (2008b). Reproductive endocrinology of the largest dasyurids: characterisation of ovarian cycles by plasma and fecal steriod monitoring. Part II. The spotted-tail quoll (Dasyurus maculatus). Gen. Comp. Endocrinol. 155, 245–254.
Reproductive endocrinology of the largest dasyurids: characterisation of ovarian cycles by plasma and fecal steriod monitoring. Part II. The spotted-tail quoll (Dasyurus maculatus).CrossRef | 1:CAS:528:DC%2BD2sXhtlGiu77N&md5=8151ad8202d6341335ed0d0db4951cd0CAS |

Hickford, D. E., Merry, N. E., Johnson, M. H., and Selwood, L. (2001). Induced ovulation, mating success and embryonic development in the stripe-faced dunnart, Sminthopsis macroura. Reproduction 122, 777–783.
Induced ovulation, mating success and embryonic development in the stripe-faced dunnart, Sminthopsis macroura.CrossRef | 1:CAS:528:DC%2BD3MXos1Ohu7o%3D&md5=ad82a6bc824a9a3ece92fd0d83fe463dCAS |

Hinds, L. A., Fletcher, T. P., and Rodger, J. C. (1996). Hormones of oestrus and ovulation and their manipulation in marsupials. Reprod. Fertil. Dev. 8, 661–672.
Hormones of oestrus and ovulation and their manipulation in marsupials.CrossRef | 1:CAS:528:DyaK28XlvVWksbo%3D&md5=2ca43f9a516d833599072498632a5ae3CAS |

Howard, J. G., and Wildt, D. E. (2009). Approaches and efficacy of artificial insemination in felids and mustelids. Theriogenology 71, 130–148.
Approaches and efficacy of artificial insemination in felids and mustelids.CrossRef | 1:STN:280:DC%2BD1cjnt1Crug%3D%3D&md5=9f5d54906c42bc0d246206f1e4a1b14fCAS |

Howard, J., Marinari, P. E., and Wildt, D. E. (2003). Black-footed ferret: model for assisted reproductive technologies contributing to in situ conservation. In ‘Reproduction Science and Integrated Conservation’. (Eds W. V. Holt, A. R. Pickard, J. C. Rodger, and D. E. Wildt.) pp. 249–266. (Cambridge University Press: Cambridge, UK.)

Hughes, R. L., and Rodger, J. C. (1971). Studies on the vaginal mucus of the marsupial Trichosurus vulpecula. Aust. J. Zool. 19, 19–33.
Studies on the vaginal mucus of the marsupial Trichosurus vulpecula.CrossRef |

Islam, R. (2011). Synchronization of estrus in cattle: a review. Vet. World 4, 136–141.
Synchronization of estrus in cattle: a review.CrossRef |

Johnston, S. D., and Holt, W. V. (2001). Germplasm conservation in marsupials. In ‘Cryobanking the Genetic Resource: Wildlife Conservation for the Future?’ (Eds P. F. Watson and W. V. Holt.) pp. 201–227. (Taylor and Francis: London.)

Johnston, S. D., O’Callaghan, P., Nilsson, K., Tzipori, G., and Curlewis, J. D. (2004). Semen-induced luteal phase and identification of a LH surge in the koala (Phascolarctos cinereus). Reproduction 128, 629–634.
Semen-induced luteal phase and identification of a LH surge in the koala (Phascolarctos cinereus).CrossRef | 1:CAS:528:DC%2BD2cXhtVSis7nK&md5=eb5afdfcb3ee147105b6a7ec9db7ac76CAS |

Jungnickel, M. K., and Hinds, L. A. (2000). Hormonal profiles in the tammar wallaby, Macropus eugenii, following FSH/LH superovulation. Reprod. Fertil. Dev. 12, 457–464.
Hormonal profiles in the tammar wallaby, Macropus eugenii, following FSH/LH superovulation.CrossRef | 1:CAS:528:DC%2BD3MXmtVOitrc%3D&md5=8963ce114b7ecbc1454ed3b758985397CAS |

Khan, K. N., Kitajima, M., Hiraki, K., Fujishita, A., Sekine, I., Ishimaru, T., and Masuzaki, H. (2010). Changes in tissue inflammation, angiogenesis and apoptosis in endometriosis, adenomyosis and uterine myoma after GnRH agonist therapy. Hum. Reprod. 25, 642–653.
Changes in tissue inflammation, angiogenesis and apoptosis in endometriosis, adenomyosis and uterine myoma after GnRH agonist therapy.CrossRef | 1:CAS:528:DC%2BC3cXitFeisbg%3D&md5=f8210be4820741e5409ef75f649815cfCAS |

Lipscomb, G. (2008). Uterine artery embolization and minimally invasive techniques for uterine fibroids. In ‘Global Library of Womens Medicine’. (Ed. S. Arulkumaran.). (The International Federation of Gynecology and Obstetrics: London.)

Lohr, C. A., Mills, H., Robertson, H., and Bencini, R. (2009). Deslorelin implants control fertility in urban brushtail possums (Trichosurus vulpecula) without negatively influencing their body-condition index. Wildl. Res. 36, 324–332.
Deslorelin implants control fertility in urban brushtail possums (Trichosurus vulpecula) without negatively influencing their body-condition index.CrossRef | 1:CAS:528:DC%2BD1MXms1agur8%3D&md5=1263e55428dff3ae7b7b6101abe58b38CAS |

Lòpez-Gatius, F., Murugavel, K., Santolaria, P., López-Béjar, M., and Yániz, J. L. (2004). Pregnancy rate after timed artificial insemination in early post-partum dairy cows after ovsynch or specific synchronization protocols. J. Vet. Med. A Physiol. Pathol. Clin. Med. 51, 33–38.
Pregnancy rate after timed artificial insemination in early post-partum dairy cows after ovsynch or specific synchronization protocols.CrossRef |

Martin, P. G. (1965). The potentialities of the fat-tailed marsupial mouse Sminthopsis crassicaudata (Gould), as a laboratory animal. Aust. J. Zool. 13, 559–562.
The potentialities of the fat-tailed marsupial mouse Sminthopsis crassicaudata (Gould), as a laboratory animal.CrossRef |

Martinez, M. F., McLeod, B., Tattersfield, G., Smaill, B., Quirke, L. D., and Juengel, J. L. (2015). Successful induction of oestrus, ovulation and pregnancy in adult ewes and ewe lambs out of the breeding season using a GnRH+progesterone oestrus synchronisation protocol. Anim. Reprod. Sci. 155, 28–35.
Successful induction of oestrus, ovulation and pregnancy in adult ewes and ewe lambs out of the breeding season using a GnRH+progesterone oestrus synchronisation protocol.CrossRef | 1:CAS:528:DC%2BC2MXivFGktbg%3D&md5=5978fd3a8f68f50fb4b98d7c17af43fbCAS |

McDonald, C. H., Taggart, D. A., Breed, W. G., Druery, G. V., Shimmin, G. A., Finlayson, G. R., and Paris, M. C. J. (2006). Effect of exogenous gonadotrophins on ovarian morphology and oocyte maturation in the southern hairy nosed wombat Lasiorhinus latifrons during the breeding season. Reprod. Fertil. Dev. 18, 477–484.
Effect of exogenous gonadotrophins on ovarian morphology and oocyte maturation in the southern hairy nosed wombat Lasiorhinus latifrons during the breeding season.CrossRef | 1:CAS:528:DC%2BD28XjtV2hurs%3D&md5=0fd1cc701a909fc3b171308d7880febeCAS |

Mizutani, T., Sugihara, A., Nakamuro, K., Suehara, N., and Terada, N. (1999). The gonadotropin-releasing hormone agonist leuprolide acetate induces apoptosis and suppresses cell proliferative activity in rectovaginal endometriosis. Am. J. Obstet. Gynecol. 181, 750–751.
The gonadotropin-releasing hormone agonist leuprolide acetate induces apoptosis and suppresses cell proliferative activity in rectovaginal endometriosis.CrossRef | 1:STN:280:DyaK1MvhslCluw%3D%3D&md5=cca17d015702bff42859aa8d0d2bdbcaCAS |

Okada, H. (1997). One- and three-month release injectable micropsheres of the LH-RH superagonist lueprorelin acetate. Adv. Drug Deliv. Rev. 28, 43–70.
One- and three-month release injectable micropsheres of the LH-RH superagonist lueprorelin acetate.CrossRef | 1:CAS:528:DyaK2sXntVOjsr0%3D&md5=15e72c8d148268d12180a6fef531aaf7CAS |

Periti, P., Mazzei, T., and Mini, E. (2002). Clinical pharmacokinetics of depot leuprorelin. Clin. Pharmacokinet. 41, 485–504.
Clinical pharmacokinetics of depot leuprorelin.CrossRef | 1:CAS:528:DC%2BD38Xmt12mtbk%3D&md5=2f9d8179e772967865c98d333cdd3c10CAS |

Pursley, J. R., Mee, M. O., and Wiltbank, M. C. (1995). Synchronisation of ovulation in dairy cows using PGF and GnRH. Theriogenology 44, 915–923.
Synchronisation of ovulation in dairy cows using PGF and GnRH.CrossRef | 1:CAS:528:DyaK2MXpvFymu7w%3D&md5=622a8817da5de304d61126f3fcace617CAS |

Rodger, J. C., Breed, W. G., and Bennett, J. H. (1992). Gonadotrophin-induced oestrus and ovulation in the polyovulatory marsupial Sminthopsis crassicaudata. Reprod. Fertil. Dev. 4, 145–152.
Gonadotrophin-induced oestrus and ovulation in the polyovulatory marsupial Sminthopsis crassicaudata.CrossRef | 1:CAS:528:DyaK38XmtVGgur4%3D&md5=1d47151c72144fe382b29d0388f19c4dCAS |

Rodger, J. C., Paris, D. B. B. P., Czarny, N. A., Harris, M. S., Molinia, F. C., Taggart, D. A., Allen, C. D., and Johnston, S. D. (2009). Artificial insemination in marsupials. Theriogenology 71, 176–189.
Artificial insemination in marsupials.CrossRef |

Ryan, D. P., Galvin, J. A., and O’Farrell, K. J. (1999). Comparison of oestrous synchronization regimens for lactating dairy cows. Anim. Reprod. Sci. 56, 153–168.
Comparison of oestrous synchronization regimens for lactating dairy cows.CrossRef | 1:CAS:528:DyaK1MXlsFyju7k%3D&md5=91c8df1fb047e78de4b0f0611fa658afCAS |

Santymire, R. (2016). Implementing the use of a biobank in the endangered black-footed ferret (Mustela nigripes). Reprod. Fertil. Dev. 28, 1097–1104.
Implementing the use of a biobank in the endangered black-footed ferret (Mustela nigripes).CrossRef | 1:CAS:528:DC%2BC28XhtVWgtbvN&md5=55a12e78396cf7ea2f38e14329135a7eCAS |

Schneider, F., Tomek, W., and Gründker, C. (2006). Gonadotropin-releasing hormone (GnRH) and its natural analogues: a review. Theriogenology 66, 691–709.
Gonadotropin-releasing hormone (GnRH) and its natural analogues: a review.CrossRef | 1:CAS:528:DC%2BD28Xot1WrtLw%3D&md5=b649f1bd57e98e00269dcf972f53432eCAS |

Selwood, L., and Woolley, P. A. (1991). A timetable of embryonic development, and ovarian and uterine changes during pregnancy, in the stripe-faced dunnart, Sminthopsis macroura (Marsupialia: Dasyuridae). J. Reprod. Fertil. 91, 213–227.
A timetable of embryonic development, and ovarian and uterine changes during pregnancy, in the stripe-faced dunnart, Sminthopsis macroura (Marsupialia: Dasyuridae).CrossRef | 1:STN:280:DyaK3M7ktVCmtQ%3D%3D&md5=cf01358ef705b9cfc045e2626613f744CAS |

Simpson, S. J., Flecknoe, S. J., Clugston, R. D., Greer, J. J., Hooper, S. B., and Frappell, P. B. (2011). Structural and functional development of the respiratory system in a newborn marsupial with cutaneous gas exchange. Physiol. Biochem. Zool. 84, 634–649.
Structural and functional development of the respiratory system in a newborn marsupial with cutaneous gas exchange.CrossRef | 1:CAS:528:DC%2BC3MXhs1ejt7zF&md5=f2a4aae52ea26b8b6482af00c76a6d73CAS |

Simpson, S. J., Fong, A. Y., Cummings, K. J., and Frappell, P. B. (2012). The ventilatory response to hypoxia and hypercapnia is absent in the neonatal fat-tailed dunnart. J. Exp. Biol. 215, 4242–4247.
The ventilatory response to hypoxia and hypercapnia is absent in the neonatal fat-tailed dunnart.CrossRef |

Smith, M. J., and Godfrey, G. K. (1970). Ovulation induced by gonadotrophins in the marsupial, Sminthopsis crassicaudata (Gould). J. Reprod. Fertil. 22, 41–47.
Ovulation induced by gonadotrophins in the marsupial, Sminthopsis crassicaudata (Gould).CrossRef | 1:CAS:528:DyaE3MXltVaisQ%3D%3D&md5=15da400a93e27e2ddc240dd2a280fa48CAS |

Smith, M. J., Bennett, J. H., and Chesson, C. M. (1978). Photoperiod and some other factors affecting reproduction in female Sminthopsis crassicaudata (Gould) (Marsupialia: Dasyuridae) in captivity. Aust. J. Zool. 26, 449–463.
Photoperiod and some other factors affecting reproduction in female Sminthopsis crassicaudata (Gould) (Marsupialia: Dasyuridae) in captivity.CrossRef |

Tarlatzis, B. C., and Kolibianakis, E. M. (2007). GnRH agonists vs antagonists. Best Pract. Res. Clin. Obstet. Gynaecol. 21, 57–65.
GnRH agonists vs antagonists.CrossRef | 1:STN:280:DC%2BD2s7jtlCktQ%3D%3D&md5=75de56b6702618eef574ff17a651a7b2CAS |

Tyndale-Biscoe, C. H., and Renfree, M. B. (1987) ‘Reproductive Physiology of Marsupials.’ (Cambridge University Press: Melbourne.)

Wiebe, V. J., and Howard, J. P. (2009). Pharmacologic advances in canine and feline reproduction. Top. Companion Anim. Med. 24, 71–99.
Pharmacologic advances in canine and feline reproduction.CrossRef |

Wilson, A. C., Meethal, S. V., Bowen, R. L., and Atwood, C. S. (2007). Leuprolide acetate: a drug of diverse clinical applications. Expert Opin. Investig. Drugs 16, 1851–1863.
Leuprolide acetate: a drug of diverse clinical applications.CrossRef | 1:CAS:528:DC%2BD2sXht1aksb%2FI&md5=4374c4558e7c422d83d7aad41258956bCAS |

Witt, R. R., Forbes, I. R., McBain, J., and Rodger, J. C. (2016). Ovarian suppression in a marsupial following single treatment with a gonadotrophin-releasing hormone agonist in microspheres. Reprod. Fertil. Dev. 28, 1964–1973.
Ovarian suppression in a marsupial following single treatment with a gonadotrophin-releasing hormone agonist in microspheres.CrossRef | 1:CAS:528:DC%2BC28Xhs1KrsLnP&md5=ddfff61b15336a342ff8df5936390612CAS |

Woodward, R., Herberstein, M. E., and Herbert, C. A. (2006). Fertility control in female eastern grey kangaroos using the GnRH agonist deslorelin. 2. Effects on behaviour. Wildl. Res. 33, 47–55.
Fertility control in female eastern grey kangaroos using the GnRH agonist deslorelin. 2. Effects on behaviour.CrossRef | 1:CAS:528:DC%2BD28XitVOhtrs%3D&md5=5212c0e41683f5fa36ffa5ca44f1f2d2CAS |



Export Citation