Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Profilin 1 plays feedback role in actin-mediated polar body extrusion in mouse oocytes

Jun Liu A , Qiao-Chu Wang A , Xing Duan A , Xiang-Shun Cui B , Nam-Hyung Kim B , Yu Zhang A and Shao-Chen Sun A C
+ Author Affiliations
- Author Affiliations

A College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

B Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Korea.

C Corresponding author. Email: sunsc@njau.edu.cn

Reproduction, Fertility and Development - https://doi.org/10.1071/RD17354
Submitted: 1 June 2017  Accepted: 30 September 2017   Published online: 3 November 2017

Abstract

Mammalian oocytes undergo several crucial processes during meiosis maturation, including spindle formation and migration and polar body extrusion, which rely on the regulation of actin. As a small actin-binding protein, profilin 1 plays a central role in the regulation of actin assembly. However, the functions of profilin 1 in mammalian oocytes are uncertain. To investigate the function of profilin 1 in oocytes, immunofluorescent staining was first used to examine profilin 1 localisation. The results showed that profilin 1 was localised around the meiotic spindles and was colocalised with cytoplasmic actin. Knockdown (KD) of profilin 1 with specific morpholino microinjection resulted in failure of polar body extrusion. This failure resulted from an increase of actin polymerisation both at membranes and in the cytoplasm. Furthermore, western blot analysis revealed that the expression of Rho-associated kinase (ROCK) and phosphorylation levels of myosin light chain (MLC) were significantly altered after KD of profilin 1. Thus, the results indicate that a feedback mechanism between profilin, actin and ROCK–MLC2 regulates actin assembly during mouse oocyte maturation.

Additional keywords: cytokinesis, meiosis.


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