Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
Table of Contents
Reproduction, Fertility and Development

Reproduction, Fertility and Development

Volume 29 Number 12 2017


Is the temperature in a preovulatory follicle lower than in adjacent tissues? Using a novel non-invasive approach it was demonstrated that bovine follicular fluid before ovulation is cooler than the uterine surface and deep rectal tissues, whereas temperature is not reduced in follicles suffering ovulation failure. Follicular antral temperature could become a more valuable indicator of oocyte potential than a purely morphological assessment.

RD16513Melatonin potentially acts directly on swine ovary by modulating granulosa cell function and angiogenesis

Giuseppina Basini, Simona Bussolati, Roberta Ciccimarra and Francesca Grasselli
pp. 2305-2312

The reproductive effects of melatonin have been extensively studied, but its involvement in the local control of ovarian physiology has not yet been clarified. The present research, which was undertaken to study the effects of physiological levels of melatonin on the main parameters of swine granulosa cell function, documents that the hormone is involved in the modulation of ovarian follicle growth and development.

RD16428Intracytoplasmic oxidative stress reverses epigenetic modifications in polycystic ovary syndrome

Fatemeh Eini, Marefat Ghaffari Novin, Khojasteh Joharchi, Ahmad Hosseini, Hamid Nazarian, Abbas Piryaei and Arash Bidadkosh
pp. 2313-2323

In PCOS, elevated levels of plasma androgens affects the quality of oocytes and ovulation rate, and causes anovulation in women of reproductive age. We studied the mechanism in a mouse model of PCOS, and showed that intracytoplasmic oxidative stress in oocyte via increase in histone acetylation and decrease in DNA methylation and histone dimethylation alters epigenetic modification. Consistently, mRNA expression of DNA methyltransferase-1 and histone deacetylase-1 was decreased with PCOS. There was a significant correlation between ROS production and increased histone acetylation. We concluded that antioxidant therapy in PCOS is a viable approach to reduce the adverse effects of epigenetic alterations.

RD16459Comparative analysis of granulosa cell gene expression in association with oocyte competence in FSH-stimulated Holstein cows

David A. Landry, Chloé Fortin, Anne-Marie Bellefleur, Rémi Labrecque, François-Xavier Grand, Christian Vigneault, Patrick Blondin and Marc-André Sirard
pp. 2324-2335

In cattle, a major limitation of the success of IVF is the developmental competence that oocytes acquire during ovarian stimulation. The aim of the present study was to better understand the follicular conditions associated higher versus lower embryo yields. We revealed that the level of follicular differentiation (timing) is the main cause of failure and provide new biomarkers to identify such follicular conditions to adapt the ovarian stimulation protocol to individual animals.

RD16343Andrographolide disrupts meiotic maturation by blocking cytoskeletal reorganisation and decreases the fertilisation potential of mouse oocytes

Hong-xing Liang, Sheng-sheng Lu, Zheng Yan, Yan-ping Kuang, Xiang-xing Zhu, Zhi-guang Yan, Tong Du, Wei-ran Chai, Hui Long and Qi-feng Lyu
pp. 2336-2344

Andrographolide should be prudently consumed by women attempting to conceive. The aim of the present study was to investigate the effects of andrographolide on oocytes. The findings indicate that andrographolide may disrupt mouse oocyte meiotic maturation and fertilisation potential by blocking cytoskeletal reorganisation. This study provides an important platform for understanding the potential adverse effects of andrographolide on female fertility.

RD16366Development of pre-implantation porcine blastocysts cultured within alginate hydrogel systems either supplemented with secreted phosphoprotein 1 or conjugated with Arg-Gly-Asp Peptide

Taylor D. Laughlin, Jeremy R. Miles, Elane C. Wright-Johnson, Lea A. Rempel, Clay A. Lents and Angela K. Pannier
pp. 2345-2356

Deficiencies in the initiation of embryo elongation play a major role in early embryonic mortality in the pig. This study demonstrates that embryos encapsulated and cultured in alginate hydrogels with the integrin-binding Arg-Gly-Asp (RGD) sequence had increased embryonic survival and modulation of gene expression to support increased steroid production and adequate immune responsiveness, with a larger number exhibiting the morphological changes compared to other in vitro treatments in this study. These results illustrate the importance of the integrin-binding RGD peptide sequence for stimulating the initiation of embryo elongation and improving embryo survival.


Porcine embryos contain substantial amounts of lipid, with little known regarding its metabolic role during development. This study investigated the association of lipid metabolism with carbohydrate substrates in the culture medium and revealed positive effects of adding L-carnitine, a limiting co-factor required for lipolysis, on early cleavage and blastocyst quality. Supplementing medium with L-carnitine is a useful strategy to improve the success of porcine blastocyst vitrification.

RD17046Protective effects of polydatin on experimental testicular torsion and detorsion injury in rats

Huilian Qiao, He Ma, Wanjun Cao, Hao Chen, Jinhua Wei and Zhen Li
pp. 2367-2375

The notable pathophysiological event that occurs in testicular torsion–detorsion (T/D) is ischaemia–reperfusion (I/R), which lacks efficient clinical treatment. Herein we describe, for the first time, the protective effects of polydatin, a traditional Chinese medicine, against testicular T/D injury in the rat. Our findings may provide new clues for male infertility associated with testicular torsion.


Binding of spermatozoa to the egg coat is a key step in fertilisation and provides a mechanism for reproductive isolation and sexual selection. To test this theory we investigated the molecular evolution of the ZP3 gene and its encoded sperm-binding region in a diverse murine rodent group. Our analyses provides evidence for positive selection on the sperm-binding region in several murine lineages, but the nature of the selective force remains unresolved.

RD16457Differential gene expression in porcine oviduct during the oestrous cycle

O. S. Acuña, M. Avilés, R. López-Úbeda, A. Guillén-Martínez, C. Soriano-Úbeda, A. Torrecillas, P. Coy and M. J. Izquierdo-Rico
pp. 2387-2399

The importance of the oviduct for different reproductive processes is known; however, the molecular mechanisms underlying oviductal regulation are not fully understood. The results of the present study demonstrate that gene expression in the oviduct is regulated during the oestrous cycle, with some oviductal proteins that could be related to several reproductive processes being described for the first time. These findings are very important in our understanding of the different processes that take place in the oviduct as fertilisation or early embryo development.

RD16398Effects of ovarian disaggregation on adult murine follicle yield and viability

Fiona Young, John Drummond, Emma Akers, Louise Bartle, David Kennedy and Mohammad Asaduzzaman
pp. 2400-2410

Enzymatic disaggregation of murine ovaries yielded higher numbers of morphologically intact follicules than previously reported. The molecular markers DAPI, CMXRos and alpha-tubulin were applied to assess post-harvest follicular morphology, mitochondrial function and cellular cytoskeleton respectively. A new preparation of animal origin-free collagenase IV caused more damage to follicular integrity, mitochondrial function and the cytoskeleton than enzyme-free mechanical isolation, but another preparation of collagenase IV ovarian disaggregation gave the highest yield of morphologically intact follicles containing viable mitochondria.

RD17111Splicing-related single nucleotide polymorphism of RAB, member of RAS oncogene family like 2B (RABL2B) jeopardises semen quality in Chinese Holstein bulls

Xiuge Wang, Xiaohui Cui, Yan Zhang, Haisheng Hao, Zhihua Ju, Deyu Liu, Qiang Jiang, Chunhong Yang, Yan Sun, Changfa Wang, Jinming Huang and Huabin Zhu
pp. 2411-2418

Bull semen quality traits are vital parameters in evaluating the fertility potential of bulls. In the present study we demonstrated that a single nucleotide polymorphism within the exonic splicing enhancer motif caused aberrant splicing of the RAB, member of RAS oncogene family like 2B (RABL2B) gene, and that semen quality differed among Chinese Holstein bulls with different RABL2B genotypes. These results also provide a novel research idea for further elucidating the complex molecular mechanism underlying the regulation of bull fertility.

RD16386Effects of vitrification of cumulus-enclosed porcine oocytes at the germinal vesicle stage on cumulus expansion, nuclear progression and cytoplasmic maturation

Ruth Appeltant, Tamás Somfai, Elisa C. S. Santos, Thanh Quang Dang-Nguyen, Takashi Nagai and Kazuhiro Kikuchi
pp. 2419-2429

Although oocyte cryopreservation is desired for the preservation of female germplasm, the developmental competence of vitrified porcine oocytes has been notoriously low due to unclear reasons. We investigated the effects of vitrification of porcine oocytes at the immature stage. Vitrification triggered premature nuclear resumption and nucleolar fragmentation from which some oocytes could recover, suggesting that enhancement of recovery processes might be a possible way to improve competency.

RD17100Mouse minipuberty coincides with gonocyte transformation into spermatogonial stem cells: a model for human minipuberty

Ruili Li, Amanda Vannitamby, Sarah S. K. Yue, David Handelsman and John Hutson
pp. 2430-2436

Subsequent infertility and testicular cancer are major problems for boys with undescended testis. This study aims to examine whether mouse has similar postnatal hormonal changes as boys, so it can be used as a model to study human testis development. Our results demonstrated that mouse can be used as a model for human minipuberty.

RD16460Regulation of interleukin-11 expression in ovulatory follicles of the rat ovary

You-Jee Jang, Jae-Il Park, Seong-Eun Jeong, You-Mi Seo, Phuong T. M. Dam, Young-Woo Seo, Bum-Chae Choi, Sang-Jin Song, Sang-Young Chun and Moon-Kyoung Cho
pp. 2437-2445

The expression of IL-11 is stimulated by LH/hCG in theca cells of preovulatory follicles via PKA-MAPK pathway and TLR4 activation. IL-11 increases progesterone production by stimulating StAR expression.

RD16290Novel insights into the role of cell-free seminal mRNAs on semen quality and cryotolerance of spermatozoa in bulls (Bos taurus)

Munivenkatappa Shilpa, Sellappan Selvaraju, Venkataswamy GirishKumar, Sivashanmugam Parthipan, Krishnan B. Binsila, Arunachalam Arangasamy and Janivara Parameshwaraiah Ravindra
pp. 2446-2456

The selection of fertile males is important to augment fertility in cows, and breeding soundness examinations may not provide information on the fertility potential of bulls. Expression levels of seminal plasma RNAs may be used to predict the fertilising ability and cryotolerance of bull semen. Such non-invasive tests using seminal plasma may aid in the assessment of the health of the reproductive tract and in the selection of males for breeding.

RD17136Effect of seminal plasma from high- and low-fertility bulls on cauda epididymal sperm function

S. A. Holden, B. Fernandez-Fuertes, E. M. Murphy, P. Lonergan and S. Fair
pp. 2457-2465

Seminal plasma bathes the sperm upon ejaculation but its function has yet to be fully defined. This study sought to characterise the effect of seminal plasma from bulls of high or low fertility on sperm function. Seminal plasma reduced osmotic resistance of epididymal sperm, regardless of whether it was from high or low fertility bulls.

RD16282Increased high molecular weight adiponectin, but decreased total adiponectin and kisspeptin, in central precocious puberty compared with aged-matched prepubertal girls

Chantacha Sitticharoon, Maynart Sukharomana, Supawadee Likitmaskul, Malika Churintaraphan and Pailin Maikaew
pp. 2466-2478

The factors initiating puberty have not been clearly revealed. The present study compared serum total and high molecular weight (HMW) adiponectin levels between girls with central precocious puberty (CPP) and age-matched controls. Total adiponectin levels were low, but HMW adiponectin levels were high in girls with CPP, which probably leads to decreased inhibition of gonadotrophin-releasing hormone neuronal activity. This may provide an insight into how adipokines are involved in the initiation of puberty.

RD16301Visfatin and resistin in gonadotroph cells: expression, regulation of LH secretion and signalling pathways

Virginie Maillard, Sébastien Elis, Alice Desmarchais, Céline Hivelin, Lionel Lardic, Didier Lomet, Svetlana Uzbekova, Philippe Monget and Joëlle Dupont
pp. 2479-2495

Resistin and visfatin, two adipokines, modulate reproduction in the gonads, but their actions at the hypothalamic–pituitary level are not known. We showed in vitro that these hormones decreased LH secretion from mouse gonadotroph cells and that in vivo visfatin may be a myokine as well as an adipokine in mice. Our findings suggest that these hormones may influence mouse female fertility by regulating LH secretion at the pituitary level.

RD17107Delayed onset of puberty in male offspring from bisphenol A-treated dams is followed by the modulation of gene expression in the hypothalamic–pituitary–testis axis in adulthood

Isabela M. Oliveira, Renata M. Romano, Patricia de Campos, Monica D. Cavallin, Claudio A. Oliveira and Marco A. Romano
pp. 2496-2505

The developing fetal brain is a potent target for endocrine chemical disruption by bisphenol A (BPA) because of its lipophilicity. In the present study, perinatal exposure to BPA changed the expression of genes regulating the hypothalamic–pituitary–testis (HPT) axis at the hypothalamic, pituitary and testes levels. Maternal exposure to BPA during fetal differentiation of the sexually dimorphic nucleus of the hypothalamus caused reprogramming of the HPT axis in adult male offspring

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