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Vertebrate reproductive science and technology
RESEARCH ARTICLE

Dose and administration protocol for FSH used for ovarian stimulation affect gene expression in sheep cumulus–oocyte complexes

Gláucia M. Bragança A D , Ribrio Ivan T. P. Batista A , Joanna Maria G. Souza-Fabjan A , Vivian A. P. Alfradique A , Eduardo K. N. Arashiro A , Isabel O. Cosentino A , Pedro Henrique N. Pinto A , Luiz Sérgio A. Camargo B , Jeferson F. da Fonseca C and Felipe Z. Brandão A
+ Author Affiliations
- Author Affiliations

A Faculdade de Veterinária, Universidade Federal Fluminense, Rua Vital Brazil, 64, CEP 24320-340, Niteroi, RJ, Brazil.

B Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa de Gado de Leite, Rua Eugênio do Nascimento, 610, CEP 36038-330, Juiz de Fora, MG, Brazil.

C Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa em Caprinos e Ovinos, Campo Experimental de Coronel Pacheco. Rodovia MG 133, km 42, CEP 36155-000, Coronel Pacheco, MG, Brazil.

D Corresponding author. Email: glauciaveterinaria@yahoo.com.br

Reproduction, Fertility and Development 30(9) 1234-1244 https://doi.org/10.1071/RD17337
Submitted: 26 August 2017  Accepted: 27 February 2018   Published: 27 March 2018

Abstract

The present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus–oocyte complexes (COCs). Twelve Santa Inês ewes (in a cross-over design) received 80 or 120 mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300 IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P > 0.05). The expression of markers of oocyte competence (ZAR1, zygote arrest 1; MATER, maternal antigen that embryo requires; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2 associated X protein) and the steroidogenic pathway (ERα, oestrogen receptor α; LHr, LH receptor; FSHr, FSH receptor; STAR, steroidogenic acute regulatory protein) was affected by stimulation. Specifically, the expression of markers of the steroidogenic pathway was reduced with increasing FSH dose in the OS protocol. FSH at a dose of 80 mg reduced the expression of FSHr and ERα in the OS versus MD protocol. Conversely, in MD protocol, only LHr was affected by increasing FSH dose. In conclusion, 80 mg FSH in the MD or OS protocol was sufficient to promote the development of multiple follicles and obtain fully grown (BCB+) oocytes. The MD protocol may be more appropriate for the production of better-quality oocytes.

Additional keywords: brilliant Cresyl blue, ewe, molecular biology, oocyte, superstimulation.


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