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Australian Systematic Botany Australian Systematic Botany Society
Taxonomy, biogeography and evolution of plants
RESEARCH ARTICLE

Molecular phylogeny and systematics of Prostanthera (Lamiaceae)

Trevor C. Wilson A B C , Barry J. Conn B and Murray J. Henwood A
+ Author Affiliations
- Author Affiliations

A School of Biological Sciences, University of Sydney, NSW 2006, Australia.

B National Herbarium of New South Wales, Mrs Macquaries Road, Sydney, NSW 2000, Australia.

C Corresponding author. Email: trevor.wilson@sydney.edu.au

Australian Systematic Botany 25(5) 341-352 https://doi.org/10.1071/SB12006
Submitted: 16 February 2012  Accepted: 1 July 2012   Published: 10 October 2012

Abstract

Prostanthera is the largest genus of Lamiaceae in Australia and was last comprehensively revised in 1870. To test the classification, and the homology of the morphological characters on which it is based, we analysed nuclear (ETS) and chloroplast (trnT–F and ndhF–rpl32) sequence data for 71 species of Westringieae (Lamiaceae) in separate and combined datasets by using maximum-parsimony and Bayesian-inference methods. Results supported the monophyly of the Westringieae, but indicated that Prostanthera is paraphyletic with respect to Wrixonia, requiring the latter to be synonymised with the former. Although combinations of datasets provided some degree of infrageneric resolution within Prostanthera sensu lato, none of its sections or series could be recovered unambiguously. Prostanthera section Prostanthera and P. section Klanderia (regarded as entomophilous and ornithophilous, respectively) did not form a sister relationship, and neither could be unequivocally resolved as monophyletic. However, all species of P. section Klanderia nested within P. section Prostanthera raising the possibility that P. section Prostanthera is paraphyletic. Similarly, the phylogeny of Prostanthera based on molecular data could not be reconciled with the morphological definition of the traditionally recognised series. We recommend abandoning Bentham’s series as a means of organising morphological variation within the genus, but acknowledge that it is premature to discard Bentham’s sections. The evolutionary and systematic implications of the lack of congruence between our molecular phylogeny and morphologically defined subgeneric taxa are discussed.


References

Bain JF, Jansen RK (2006) A chloroplast DNA hairpin structure provides useful phylogenetic data within tribe Senecioneae (Asteraceae). Canadian Journal of Botany 84, 862–868.
A chloroplast DNA hairpin structure provides useful phylogenetic data within tribe Senecioneae (Asteraceae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XpvVOrurw%3D&md5=46c8a8697dc334b4abb67923fea68a83CAS |

Baldwin BG, Markos S (1998) Phylogenetic utility of the external transcribed spacer (ETS) of 18S–26S rDNA: congruence of ETS and ITS trees of Calycadenia (Compositae). Molecular Phylogenetics and Evolution 10, 449–463.
Phylogenetic utility of the external transcribed spacer (ETS) of 18S–26S rDNA: congruence of ETS and ITS trees of Calycadenia (Compositae).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXhtlKmtb4%3D&md5=a248a743ecae3551e18f56c946932433CAS |

Barker FK, Lutzoni FM (2002) The utility of the incongruence length difference test. Systematic Biology 51, 625–637.
The utility of the incongruence length difference test.Crossref | GoogleScholarGoogle Scholar |

Bentham G (1870) Labiatae. In ‘Flora Australiensis. Vol. 5’. pp. 70–137. (Reeve: London)

Cantino PD (1992) Evidence for a polyphyletic origin of the Labiatae. Annals of the Missouri Botanical Garden 79, 361–379.
Evidence for a polyphyletic origin of the Labiatae.Crossref | GoogleScholarGoogle Scholar |

Carrick J (1976) Studies in Australian Lamiaceae 1. The genus Wrixonia (Prostantheroideae). Journal of the Adelaide Botanic Gardens 1, 27–34.

Conn BJ (1984) A taxonomic revision of Prostanthera Labill. section Klanderia (F.v. Muell.) Benth. (Labiatae). Journal of the Adelaide Botanic Gardens 6, 207–348.

Conn BJ (1988) A taxonomic revision of Prostanthera Labill. section Prostanthera Labiatae. 1. The species of the Northern Territory, South Australia and Western Australia. Nuytsia 6, 351–411.

Conn BJ (1992a) Lamiaceae. In ‘Flora of New South Wales. Vol. 3’. (Ed. GJ Harden) pp. 623–664. (Royal Botanic Gardens and Domain Trust: Sydney)

Conn BJ (1992b) Relationships within the tribe Prostanthereae (Labiatae). In ‘Advances in labiate science’. (Eds RM Harley, T Reynolds) pp. 55–64. (Royal Botanic Gardens, Kew: London)

Conn BJ (1997) Four rare and/or threatened new species of Prostanthera section Prostanthera (Lamiaceae) from New South Wales. Telopea 7, 231–244.

Conn BJ (1998) Contributions to the systematics of Prostanthera (Labiatae) in south-eastern Australia. Telopea 7, 319–332.

Conn BJ (2004) IV. Subfam. Prostantheroideae. In ‘VII. Flowering plants–Dicotyledons, Lamiales (except Acanthaceae including Avicenniaceae)’. (Ed. JW Kadereit) pp. 204–209. (Springer: Berlin)

Conn BJ (2006) New species of Prostanthera section Prostanthera (Labiatae) from New South Wales. Telopea 11, 252–259.

Conn BJ, Whiffin T (1987) A study of the variation within and between Prostanthera monticola and Prostanthera walteri Labiatae using leaf volatile oils. Muelleria 6, 375–382.

Conn BJ, Wilson TC (2012) Prostanthera tallowa: a new species from New South Wales, Australia. Telopea 14, 5–8.
Prostanthera tallowa: a new species from New South Wales, Australia.Crossref | GoogleScholarGoogle Scholar |

Conn BJ, Streiber N, Brown EA, Henwood MJ, Olmstead RG (2009) Infrageneric phylogeny of Chloantheae (Lamiaceae) based on chloroplast ndhF and nuclear ITS sequence data. Australian Systematic Botany 22, 243–256.
Infrageneric phylogeny of Chloantheae (Lamiaceae) based on chloroplast ndhF and nuclear ITS sequence data.Crossref | GoogleScholarGoogle Scholar |

Conn BJ, Henwood MJ, Streiber N (2011) Synopsis of the tribe Chloantheae and new nomenclatural combinations in Pityrodia s.lat. (Lamiaceae). Australian Systematic Botany 24, 1–9.
Synopsis of the tribe Chloantheae and new nomenclatural combinations in Pityrodia s.lat. (Lamiaceae).Crossref | GoogleScholarGoogle Scholar |

Guerin GR (2008) Evidence for polyphyly in Hemigenia and Microcorys (Lamiaceae: Westringieae). Australian Systematic Botany 21, 313–325.
Evidence for polyphyly in Hemigenia and Microcorys (Lamiaceae: Westringieae).Crossref | GoogleScholarGoogle Scholar |

Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.

Henwood MJ, Lu-Irving P, Perkins AJ (2010) Can molecular systematics provide insights into aspects of the reproductive biology of Trachymene Rudge (Araliaceae)? Plant Diversity and Evolution 128, 85–110.
Can molecular systematics provide insights into aspects of the reproductive biology of Trachymene Rudge (Araliaceae)?Crossref | GoogleScholarGoogle Scholar |

Huelsenbeck P, Ronquist F (2001) Mr Bayes: bayesian inference of phylogenetic trees. Bioinformatics 17, 754–755.
Mr Bayes: bayesian inference of phylogenetic trees.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3MvotV2isw%3D%3D&md5=36cced4192c095ad0b58c7c43beb4c9bCAS |

Huelsenbeck P, Bull JJ, Cunningham CW (1996) Combining data in phylogenetic analysis. Trends in Ecology & Evolution 11, 152–158.
Combining data in phylogenetic analysis.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3M7itFCitg%3D%3D&md5=084747a5d9965c382a550830e8d38bacCAS |

Hunter JT, Williams JB, Conn BJ (2006) Rediscovery of Prostanthera staurophylla F.Muell. and reinstatement of P. teretifolia Maiden & Betche (Lamiaceae). Telopea 11, 117–126.

Keighery GJ (1980) Bird pollination in south-western Australia: a checklist. Plant Systematics and Evolution 135, 171–176.
Bird pollination in south-western Australia: a checklist.Crossref | GoogleScholarGoogle Scholar |

Keighery GJ (1982a) Bird-pollinated plants in Western Australia. In ‘Pollination and evolution’. (Eds J Armstrong, J Powell, A Richards) pp. 77–89. (Royal Botanic Gardens: Sydney)

Keighery GJ (1982b) Pollination syndromes and breeding systems of Western Australian arid zone plants. In ‘Evolution of the flora and fauna of arid Australia’. (Eds WR Barker, PJM Greenslade) pp. 167–172. (Peacock Publications: Adelaide)

Kelchner SA (2000) The evolution of non-coding chloroplast DNA and its application in plant systematics. Annals of the Missouri Botanical Garden 87, 482–498.
The evolution of non-coding chloroplast DNA and its application in plant systematics.Crossref | GoogleScholarGoogle Scholar |

Larget B, Simon DL (1999) Markov Chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16, 750–759.
Markov Chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXjslGitb8%3D&md5=118c8cc24f008e9e607abb5f8296feafCAS |

Lee MSY (2001) Uninformative characters and apparent conflict between molecules and morphology. Molecular Biology and Evolution 18, 676–680.
Uninformative characters and apparent conflict between molecules and morphology.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXis1eitro%3D&md5=f6b23b81f8251dfdd06669ef24f76816CAS |

Mueller FJHv (1876) Wrixonia. In ‘Fragmenta Phytographiae Australiae’. (Government Printer: Melbourne)

Müller K (2005) SeqState: primer design and sequence statistics for phylogenetic DNA datasets. Applied Bioinformatics 4, 65–69.

Olmstead RG, Reeves PA, Lepschi BJ (1998) Confirmation of a monophyletic Chloanthoideae (Lamiaceae) comprising tribes Chloantheae and Prostanthereae. Lamiales Newsletter 6, 7–10.

Posada D, Crandall KA (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14, 817–818.
Modeltest: testing the model of DNA substitution.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXktlCltw%3D%3D&md5=3d05fcb80b92fac912ffefaee08f7e4aCAS |

Simmons MP, Ochoterena H (2000) Gaps as characters in sequence-based phylogenetic analyses. Systematic Biology 49, 369–381.
Gaps as characters in sequence-based phylogenetic analyses.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD38zntlKjtg%3D%3D&md5=af35b411a7d6eeeb500b40be8904d80fCAS |

Shaw J, Lickey EB, Schilling EE, Small RL (2007) Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in angiosperms: the tortoise and the hare III. American Journal of Botany 94, 275–288.
Comparison of whole chloroplast genome sequences to choose noncoding regions for phylogenetic studies in angiosperms: the tortoise and the hare III.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXktFOjsLg%3D&md5=173f3147f19074042486bac04c90c492CAS |

Swofford DL (2002) ‘PAUP*: phylogenetic analysis using parsimony (*and other methods) Version 4.0b10.’ (Sinauer Associates: Sunderland, MA)

Taberlet P, Ludovic G, Guy P, Jean B (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology 17, 1105–1109.
Universal primers for amplification of three non-coding regions of chloroplast DNA.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38Xhslel&md5=d612c2384eaa3676300663657c3536fdCAS |

Tay ML, Meudt HM, Garnock-Jones PJ, Ritchie PA (2010) DNA sequences from three genomes reveal multiple long-distance dispersals and non-monophyly of sections in Australasian Plantago (Plantaginaceae). Australian Systematic Botany 23, 47–68.
DNA sequences from three genomes reveal multiple long-distance dispersals and non-monophyly of sections in Australasian Plantago (Plantaginaceae).Crossref | GoogleScholarGoogle Scholar |

Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876–4882.
The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXntFyntQ%3D%3D&md5=94a865fec9a120dfe38c896f6c3c029eCAS |

Williams ML, Drinnan AN, Walsh NG (2006) Variation within Prostanthera spinosa (Lamiaceae): evidence from morphological and molecular studies. Australian Systematic Botany 19, 467–477.
Variation within Prostanthera spinosa (Lamiaceae): evidence from morphological and molecular studies.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtVyqt77E&md5=1081064708a695c7599e742a1460ac0dCAS |

Wilson TC (2010) The evolution of pollination in Prostanthera (Labill.). PhD Thesis, University of Sydney, Sydney.

Wilson TC, Henwood MJ, Conn BJ (2012) Status of the genus Wrixonia F.Muell. (Lamiaceae). Telopea 14, 1–3.
Status of the genus Wrixonia F.Muell. (Lamiaceae).Crossref | GoogleScholarGoogle Scholar |

Yoder AD, Irwin JA, Payseur BA (2001) Failure for the ILD to determine data combinability for slow loris phylogeny. Systematic Biology 50, 408–424.
Failure for the ILD to determine data combinability for slow loris phylogeny.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD38zntVOntg%3D%3D&md5=f9df3b3e1a7f31f912f18fd0403089edCAS |