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Australian Journal of Zoology Australian Journal of Zoology Society
Evolutionary, molecular and comparative zoology
RESEARCH ARTICLE

Isolation and characterisation of 16 novel polymorphic microsatellite loci for the Eurasian coot (Fulica atra)

Tianshu Lv A C , Wanchao Zhu A C , Shuyu Xue A , Huanxin Zhang B , Xiaodong Gao A , Lidong Wang A and Honghai Zhang A D
+ Author Affiliations
- Author Affiliations

A College of Life Science, Qufu Normal University, Qufu, QF 273100, China.

B College of Marine Life Sciences, Ocean University of China, Qingdao, QD 266100, China.

C These authors contributed equally to this work.

D Corresponding author. Email: zhanghonghai67@126.com

Australian Journal of Zoology 64(6) 430-433 https://doi.org/10.1071/ZO16077
Submitted: 31 October 2016  Accepted: 24 March 2017   Published: 13 April 2017

Abstract

To study the genetic diversity of the Eurasian coot (Fulica atra), 16 novel microsatellites were isolated and characterised using a (AC)12-enriched genomic library. Polymorphism of each locus was detected in 19 individuals from Weishan Lake and Zhaoyang Lake, both located in Shandong Province, China. These microsatellite loci revealed 3–12 alleles in two populations. One locus (Fa6) showed significant deviations from Hardy–Weinberg equilibrium in the Zhaoyang Lake population. By means of Principal Coordinates Analysis, 19 individuals were divided into two groups, which verified they were from two populations. These novel microsatellites will be useful for further studies and genetic analyses of the Eurasian coot.

Additional keyword: genetic diversity.


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