Gender-bending aubergines: molecular phylogenetics of cryptically dioecious Solanum in Australia

The authors thank David E. Symon for providing specimens, discussion and advice; Nicholas Tippery, Norm Wickett, Hilary McManus and Michael Moody for help with laboratory-based aspects of this work; David E. Symon, William R. Barker, Hellmut Toelken and Kym Brennan for extensive field assistance; and Kent Holsinger, Bernard Goffinet, Brigid O’Donnell, Steve Langdon and two anonymous reviewers for comments on the manuscript. Funding was provided by the National Geographic Society, the Elizabeth DeViney Foundation, the American Society of Plant Taxonomists, the Botanical Society of America, the University of Connecticut Center for Conservation and Biodiversity, the University of Connecticut Department of Ecology and Evolutionary Biology, Sigma Xi, PBI Solanum, and the US National Science Foundation.

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Appendix 1.  List of herbarium vouchers for specimens used for DNA extraction and amplification of the trnK–matK region, plus GenBank accession codes [in brackets]
DNA codes beginning with ‘C’ are deposited at CONN. DNA codes beginning with ‘AU’ are from sheets held at AD and loaned to CTM at CONN. See Martine et al. (2006) for voucher information for ITS sequences

Appendix 2.  Primers used to amplify the trnK–matK region
All primer dilutions made from stock held in the Les Laboratory, Department of Ecology and Evolutionary Biology, University of Connecticut. Primers designed by Don Les (DL codes) or Nic Tippery (NT codes), unless noted otherwise