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Soil, land care and environmental research
RESEARCH ARTICLE

Assessment of nitrogen losses from urea and an organic manure with and without nitrification inhibitor, dicyandiamide, applied to lettuce under glasshouse conditions

Janice Asing A D , S. Saggar B , Jagrati Singh B and Nanthi S. Bolan C

A Institute of Natural Resources, Massey University, New Zealand.

B Landcare Research, Palmerston North, New Zealand.

C Centre for Environmental Risk Assessment and Remediation (CERAR), University of South Australia, Mawson Lakes, SA 5095, Australia.

D Corresponding author. Email: J.Asing@massey.ac.nz

Australian Journal of Soil Research 46(7) 535-541 https://doi.org/10.1071/SR07206
Submitted: 27 November 2007  Accepted: 26 May 2008   Published: 8 October 2008

Abstract

Urea and organic manures such as ‘Garden galore’ (GG) are used to supply nitrogen (N) in vegetable farming and floriculture systems in New Zealand. However, a significant amount of the applied N is lost to the atmosphere via nitrous oxide (N2O) and ammonia (NH3) emissions, and leached to surface and ground water as nitrate (NO3) contributing to environmental degradation such as global warming and eutrophication. One of the mitigation options to reduce these losses is to use nitrification inhibitors (NI).

Glasshouse and laboratory incubation experiments were conducted under controlled moisture and temperature conditions to determine the effects of an NI, dicyandiamide (DCD), on N losses from urea and GG applied to lettuce grown in a Manawatu sandy soil. Nitrogen and DCD were applied at the rates of 9 and 1.3 g/m2, respectively, and the gaseous emission of N2O and NH3 were monitored over a 5-week period using a closed-chamber technique. At the end of the experiment the lettuce plant shoots and roots were harvested, and analysed for N concentration. Soils were leached with deionised water and leachates were analysed for ammonium (NH4+) and NO3.

The results showed greater loss of N as NH3 than N2O and the effect was more pronounced in the case of urea. Addition of DCD significantly reduced N2O emissions from both urea and GG, and increased NH3 emissions from both urea and GG, with the increase being significant only for urea. Addition of DCD maintained higher soil NH4+ concentration and lower NO3 concentration than without DCD. Overall, DCD was effective in reducing N losses of N2O emissions and NO3 leaching. Urea application resulted in shoot tip burning and the symptoms were enhanced with the addition of DCD. There was no significant effect of DCD addition on lettuce yield.

Additional keywords: ammonia, DCD, Garden galore, nitrification inhibitor, nitrate leaching, nitrous oxide, ammonium, nitrate.


Acknowledgement

The authors thank Landcare Research, Palmerston North, New Zealand, for equipment and analytical facilities.


References


Atkinson CJ (1985) Nitrogen acquisition in four coexisting species from an upland acidic grassland. Journal of Plant Physiology 63, 375–387.
CrossRef | open url image1

Belastegui Macadam XM, del Prado A, Merino P, Estavillo JM, Pinto M, Gonzalez-Murua C (2003) Dicyandiamide and 3,4-dimethyl pyrazole phosphate decrease N2O emissions from grassland but dicyandiamide produces deleterious effects in clover. Journal of Plant Physiology 160, 1517–1523.
CrossRef | PubMed | open url image1

Blacquiere T, Hofstra R, Stulen I (1987) Ammonium and nitrate nutrition in Plantago lanceolata L. and Plantago major L. ssp. major. III. Nitrogen metabolism. Plant and Soil 104, 129–141.
CrossRef | open url image1

Blakemore LC , Searle PL , Daly BK (1987) Methods for chemical analysis of soils. New Zealand Soil Bureau Scientific Report No. 80.

Bolan NS, Saggar S, Luo J, Bhandral R, Singh J (2004) Gaseous emissions of nitrogen from grazed pastures: processes, measurements and modeling, environmental implications, and mitigation. Advances in Agronomy 84, 37–120.
CrossRef | open url image1

Bouwman AF (1990) Exchange of greenhouse gases between terrestrial ecosystems and the atmosphere. In ‘Soils and the greenhouse effect’. (Ed. AF Bouwman) pp. 66–127. (John Wiley & Sons Ltd: Chichester, UK)

Boxman AW, Krabbendam H, Bellemakers MJS, Roelofs JGM (1991) Effects of ammonium and aluminium on the development and nutrition of Pinus nigra in hydroculture. Environmental Pollution 73, 119–136.
CrossRef | PubMed | open url image1

Chaillou S, Morot-Gaudry JF, Salsac L, Lesaint C, Jolivet E (1986) Compared effects of NO3 – or NH4 + on growth and metabolism of French bean. Physiologie Vegetale 27, 679–687. open url image1

Cookson WR, Cornforth IS (2002) Dicyandiamide slows nitrification in dairy cattle urine patches: effects on soil solution composition, soil pH and pasture yield. Soil Biology & Biochemistry 34, 1461–1465.
CrossRef | open url image1

Crutzen PJ (1981) Atmospheric chemical process of the oxides of nitrogen including nitrous oxide. In ‘Denitrification, nitrification and atmospheric nitrous oxide’. (Ed. CC Delwiche) pp. 17–44. (John Wiley & Sons: New York)

de Graaf MCC, Bobbink R, Verbeek PM, Roelofs JGM (1998) Differential effects of ammonium and nirate on three healthland species. Plant Ecology 135, 185–196.
CrossRef | open url image1

Di HJ, Cameron KC (2002) The use of a nitrification inhibitor, dicyandiamide (DCD), to decrease nitrate leaching and nitrous oxide emissions in a simulated grazed and irrigated grassland. Soil Use and Management 18, 395–403.
CrossRef | open url image1

Di HJ, Cameron KC (2003) Mitigation of nitrous oxide emissions in spray-irrigated grazed grassland by treating the soil with dicyandiamide, a nitrification inhibitor. Soil Use and Management 19, 284–290.
CrossRef | open url image1

Di HJ, Cameron KC (2004) Treating grazed pasture soil with a nitrification inhibitor, eco-nTM, to decrease nitrate leaching in a deep sandy soil under spray irrigation – a lysimeter study. New Zealand Journal of Agricultural Research 47, 351–361. open url image1

Di HJ, Cameron KC (2005) Reducing environmental impacts of agriculture by using a fine particle suspension nitrification inhibitor to decrease nitrate leaching from grazed pastures. Agriculture, Ecosystems & Environment 109, 202–212.
CrossRef | open url image1

Dobbie KE, Smith KA (2003) Impact of different forms of N fertilizer on N2O emissions from intensive grassland. Nutrient Cycling in Agroecosystems 67, 37–46.
CrossRef | open url image1

Fox RH, Bandel VA (1989) Dicyandiamide (DCD) research in agriculture in the mid-Atlantic region. Communications in Soil Science and Plant Analysis 20, 1957–1968. open url image1

Gameh MA, Angle JS, Axley JH (1990) Effects of urea-potassium chloride and nitrogen transformations on ammonia volatilization from urea. Soil Science Society of America Journal 54, 1768–1772. open url image1

Gioacchini P, Nastri A, Marzadori C, Giovannini C, Antisari LV, Gessa C (2002) Influence of urease and nitrification inhibitors on N losses from soils fertilized with urea. Biology and Fertility of Soils 36, 129–135.
CrossRef | open url image1

Hatch D, Trindade H, Cardenas L, Carneiro J, Hawkins J, Scholefield D, Chadwick D (2005) Laboratory study of the effects of two nitrification inhibitors on greenhouse gas emissions from a slurry-treated arable soil: impact of diurnal temperature cycle. Biology and Fertility of Soils 41, 225–232.
CrossRef | open url image1

Havlin JL , Beaton JD , Tisdale SL , Nelson WL (2005) ‘Soil fertility and fertilisers. An introduction to nutrient management.’ (Prentice Hall: New Jersey)

Hewitt AE (1998) New Zealand soil classification. Landcare Research Science Series 1.

Magalhaes JR, Wilcox GE (1984a) Ammonium toxicity development in tomato plants relative to nitrogen form and light intensity. Journal of Plant Nutrition 7, 1477–1496. open url image1

Magalhaes JS, Wilcox GE (1984b) Growth, free amino acids, and mineral composition of tomato plants relative to nitrogen form and growing media. Journal of the American Society for Horticultural Science 109, 406–411. open url image1

McKenzie HA, Wallace HS (1954) The Kjeldahl determination of nitrogen: A critical study digestion conditions—temperature, catalyst, and oxidizing agent. Australian Journal of Chemistry 7, 55–70. open url image1

Merino P, Estavillo JM, Graciolli LA, Pinto M, Lacuesta M, Munoz-Rueda A, Gonzalez-Murua C (2002) Mitigation of N2O emissions from grassland by nitrification inhibitor and Actilith F2 applied with fertilizer and cattle slurry. Soil Use and Management 18, 135–141. open url image1

Mosier AR, Duxbury JM, Freney JR, Heinemeyer O, Minami K (1998) Assessing and mitigating N2O emissions from agricultural soils. Climatic Change 40, 7–38.
CrossRef | open url image1

Prakasa Rao EVS, Puttanna K (1987) Nitrification and ammonium volatilisation losses from urea and dicyandiamide-treated urea in sandy loam soil. Plant and Soil 97, 201–206.
CrossRef | open url image1

Prasad R, Power JF (1995) Nitrification inhibitors for agriculture, health, and the environment. Advances in Agronomy 54, 233–281.
CrossRef | open url image1

Puttanna K, Nanje Gowda NM, Prakash R (2001) Regulation of nitrification by benzotriazole, o-Nitrophenol, m-Nitroaniline and dicyandiamide and pattern of NH3 emissions from citronella and field fertilized with urea. Water, Air, and Soil Pollution 131, 11–17.
CrossRef | open url image1

Saggar S, Andrew RM, Tate KR, Hedley CB, Rodda NJ, Townsend JA (2004) Modelling nitrous oxide emissions from dairy-grazed pastures. Nutrient Cycling in Agroecosystems 68, 243–255.
CrossRef | open url image1

Saggar S, Hedley CB, Giltrap DL, Lambie SM (2007) Measured and modelled estimates of nitrous oxide emission and methane consumption from a sheep-grazed pasture. Agriculture, Ecosystems & Environment 122, 357–365.
CrossRef | open url image1

SAS (2003) ‘SAS package.’ (SAS Institute: Cary, NC)

Singh J (2007) The role of inhibitors in mitigating nitrogen losses from cattle urine and nitrogen fertiliser inputs in pastures. Massey University, New Zealand.

Singh J , Bolan N , Saggar S (2003) A method for simultaneous measurement of ammonia volatilization and nitrous oxide emissions from intact soil cores In ‘Applications to agriculture and environmental quality’. Palmerston North, New Zealand. (Eds LD Currie, JA Hanly) pp. 224–231. (Fertilizer and Lime Research Centre, Massey University: Palmerston North, New Zealand)

Statistics NZ (2006) ‘Fertiliser use and the environment.’ (Statistics New Zealand: Wellington, New Zealand)

Vogeler I, Blard A, Bolan N (2007) Modelling DCD effect on nitrate leaching under controlled conditions. Australian Journal of Soil Research 45, 310–317.
CrossRef | open url image1

Wang FL, Alva AK (1996) Leaching of nitrogen from slow-release urea sources in sandy soils. Soil Science Society of America Journal 60, 1454–1458. open url image1

Wang X, Below FE (1996) Cytokinins in enhanced growth and tillering of wheat induced by mixed nitrogen source. Crop Science 36, 121–126. open url image1

Williamson JC, Jarvis SC (1997) Effect of dicyandiamide on nitrous oxide flux following return of animal excreta to grassland. Soil Biology & Biochemistry 29, 1575–1578.
CrossRef | open url image1

Wulf S, Maeting M, Bergmann S, Clemens J (2001) Simultaneous measurement of NH3, N2O and CH4 to assess efficiency of trace gas emission abatement after slurry application. Phyton 41, 131–142. open url image1

Zaman M, Di HJ, Cameron KC, Frampton CM (1999) Gross nitrogen mineralization and nitrification rates and their relationships to enzyme activities and the soil microbial biomass in soils treated with dairy shed effluent and ammonium fertilizer at different water potentials. Biology and Fertility of Soils 29, 178–186.
CrossRef | open url image1

Zaman M, Nguyen ML, Blennerhassett JD (2007) Reducing NH3, N2O and NO3-N losses from a pasture soil with urease or nitrification inhibitors and elemental S-amended nitrogenous fertilizers. Biology and Fertility of Soils 44, 693–705.
CrossRef | open url image1

Zerulla W, Barth T, Dressel J, Erhardt K, von Locquenghien KH, Pasda G, Rädle M, Wissemeier A (2001) 3,4-Dimethylpyrazole phosphate (DMPP)—a new nitrification inhibitor for agriculture and horticulture. An introduction. Biology and Fertility of Soils 34, 79–84.
CrossRef | open url image1








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