CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Reproduction, Fertility and Development   
Reproduction, Fertility and Development
  Vertebrate Reproductive Science & Technology
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
Content
All Issues
Special Issues
Research Fronts
Sample Issue
For Authors
General Information
Instructions to Authors
Submit Article
Open Access
For Referees
Referee Guidelines
Review an Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our email Early Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter youtube

red arrow Connect with SRB
blank image
facebook TwitterIcon

Affiliated Societies

RFD is the official journal of the International Embryo Transfer Society and the Society for Reproductive Biology.



Article << Previous     |     Next >>   Contents Vol 22(1)

14 DEEP INTRAUTERINE FIXED-TIME ARTIFICIAL INSEMINATION USING SEXED SEMEN IN HOLSTEIN HEIFERS

G. M. Brogliatti A, G. Dominguez C, M. G. Lüssenhoff A, J. Perkins B and G. A. D

A Centro de Inseminacion La Argentina Chica, Venado Tuerto, Santa Fe, Argentina;
B Adeco SA,
C DVM Practitioner, Venado Tuerto, Santa Fe, Argentina;
D IRAC, Cordoba, Argentina
   

Abstract
Export Citation
Print
  


Abstract

Flow sorting cytometry has been shown to repeatedly produce viable sexed sperm at a level necessary for commercialization. Previous reports have shown that pregnancies rates were lower using sexed semen than commercial nonsexed semen. Sperm concentration, acrosome activation, and time to ovulation are some factors explaining the low conception. The objective of the present study was to compare pregnancy rates using sexed semen (3 × 106 sperm) deposited in different locations of the bovine uterus (body v. horn) and inseminated (AI) at 2 different times (52 h v. 58 h after progesterone intravaginal device removal). Holstein heifers, between 15 to 18 mo of age and a body condition score 3.05 ± 0.22 were used. All heifers were evaluated by transrectal ultrasonography to determine ovarian structures (284/357, 79.5% had a CL). On Day 0, all heifers received an intravaginal progesterone (P4) device (1.9 g of P4, CIDR, Pfizer, Buenos Aires, Argentina) plus 70 μg of D-cloprostenol (Bioprost, Biotay, Argentina) i.m. and 2 mg of estradiol benzoate (EB, Syntex SA, Buenos Aires, Argentina). On Day 8, CIDR devices were removed and heifers received 150 μg of D-cloprostenol i.m. at the same time. On Day 9, all heifers received 1 mg of EB i.m. and were randomly allocated in 4 different groups (2 × 2 factorial): group Body-52 h, AI in the body of the uterus at 52 h (n = 98); group Body-58 h: AI in the body of the uterus at 58 h (n = 104); group Horn-52 h: AI in the ipsilateral horn of the ovulatory follicle (detected by ultrasonography) at 52 h (n = 90) and group Horn-58 h: AI in the ipsilateral horn to the ovulatory follicle at 58 h (n = 65). All heifers were inseminated with only one dose of sexed semen. At time of AI an ultrasound examination was done to determine the size and the location of the preovulatory follicle. AI was done by a single inseminator using a traditional AI gun, and an embryo transfer gun (ET sheath, SBS Cryotec SA, Argentina) was used for deep AI. Pregnancy diagnosis was evaluated by transrectal ultrasonography using a 7.5-MHz transducer (Mindray 6600) 30 days after AI. Data were analyzed by logistic regression. Sixty percent of the preovulatory follicles were on the right ovary, and there were no differences between the sizes of the preovulatory follicle among groups (overall mean ± SEM: 15.5 ± 1.0 mm). Although pregnancy rates did not differ between horn (64/155, 41.2%) and body (70/202, 34.3%; P < 0.1) inseminations and between 52 h (67/188, 36.7% and 58 h (67/169, 39.6%), pregnancy rates were higher (P < 0.05) in heifers inseminated in the horn at 58 h (32/65, 49.2%) than those inseminated in the body at 58 h (35/104, 33.6%) and tended (P < 0.09) to be higher than heifers inseminated in the horn at 52 h (32/90, 35.5%) and in the body at 52 h (35/98, 35.7%). Fixed-time artificial insemination using ultrasonography and deep insemination could contribute to enhanced pregnancy rates using sexed semen in Holstein heifers.


This research was done with the support of ADECO SA, SBS Cryo Tec SA.

Reproduction, Fertility and Development 22(5305) 165–165   http://dx.doi.org/10.1071/RDv22n1Ab14
Published online: 08 December 2009




 
Top  Email this page
 
   


Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2014