CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Reproduction, Fertility and Development   
Reproduction, Fertility and Development
  Vertebrate Reproductive Science & Technology
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
Content
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Sample Issue
For Authors
General Information
Instructions to Authors
Submit Article
Open Access
For Referees
Referee Guidelines
Review an Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our email Early Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter youtube

red arrow Connect with SRB
blank image
facebook TwitterIcon

Affiliated Societies

RFD is the official journal of the International Embryo Transfer Society and the Society for Reproductive Biology.



Article << Previous     |     Next >>   Contents Vol 22(1)

310 SEASON INFLUENCE UPON SEMINAL PLASMA PROTEINS IN BROWN SWISS BULLS

M. G. M. Chacur A, F. P. Sirchia A, A. C. L. Ruiz A and M. L. Guaberto A

University of San Paul West, Presidente Prudente, Brazil
   

Abstract
Export Citation
Print
  


Abstract

Seminal plasma is a complex of secretions of the male accessory reproductive organs and appears to exert important effects on sperm function (Shivaji et al. 1990 Proteins of Seminal Plasma, Wiley, New York, NY, USA). The protein quality of the seminal plasma may affect positively the bulls’fertility (Killiam et al. 1993 Biol. Reprod. 49, 1202-1207). Peptides of 55 and 66 kDa were present in bulls with excellent spermatic conditions for example motility and vigor. On the other hand, 16- and 36-kDa peptides were observed with unfavorable spermatic conditions (Chacur et al. 2009 Anim. Reprod. 6, 339). The objective was to determine the influence of season on seminal plasma proteins in Brown Swiss bulls. Semen from 33 Brown Swiss bulls 24 months of age was collected by electroejaculation during winter (from June to August) and summer (from December to February) in the southern hemisphere in 2008. Semen samples were collected with 14-day intervals totalizing 196 ejaculates. Samples of semen were centrifuged (1500g/15 min) and the seminal plasma was conditioned in cryotubes and stored at -20°C until further processing. Proteins were extracted from 200 μL of each sample in 2 mL of extraction buffer composed of 0.625 M Tris-HCl, at pH 6.8, in 2% SDS, 5% fi-mercaptoethanol, and 20% of glycerol. Percentages of different plasma proteins by season were statistically compared by the chi-square test with significance level at P < 0.05). Proteins were quantified according to Bradford (1976 Anal. Biochem. 72, 248-254) and electrophoresis was performed according to Laemmili (1970 Nature 227, 680-685). Gels were fixed with isopropanol: acetic acid: water (4:1:5 v/v) for 30 minutes and stained in the same solution with 2% of Coomassie Blue R250. In 26 bulls, the absence of high molecular weight (HMW; 55 kDa, 66 kDa, and 80 kDa) proteins was found in the summer. There was a significant increase (P < 0.05) in total spermatic defects, acrosome defects, and distal cytoplasmatic droplets in these bulls. The 40-kDa protein that reflected low fertility was observed in 10 bulls in the summer with semen quality decreases. The 11 bulls showed presence of HMW (55 kDa) in the winter. In 11 bulls, HMW (55 kDa, 66 kDa, or 80 kDa) proteins were present with a satisfactory semen condition according to Killiam et al. (1993 Biol. Reprod. 49, 1202-1207). In conclusion, the seasons of the year may influence the presence of proteins in seminal plasma. There was a direct relationship of the season with seminal plasma proteins. The presence of the proteins of 20 kDa, 55 kDa, 66 kDa, and 80 kDa suggested an increase of the semen quality during the winter.


UNOESTE and Bartira Farm.

Reproduction, Fertility and Development 22(5305) 311–311   http://dx.doi.org/10.1071/RDv22n1Ab310
Published online: 08 December 2009




 
Top  Email this page
 
   


Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2014