CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Reproduction, Fertility and Development   
Reproduction, Fertility and Development
  Vertebrate Reproductive Science & Technology
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
Content
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Sample Issue
For Authors
General Information
Instructions to Authors
Submit Article
Open Access
For Referees
Referee Guidelines
Review Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our email Early Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter youtube

red arrow Connect with SRB
blank image
facebook TwitterIcon

Affiliated Societies

RFD is the official journal of the International Embryo Transfer Society and the Society for Reproductive Biology.



Article << Previous     |     Next >>   Contents Vol 22(1)

330 ROLE OF STAT3 PATHWAY IN THE LEPTIN-INDUCED SIGNALING DURING OOCYTE IN VITRO MATURATION AND STEROIDOGENIC RESPONSE IN RABBIT MODEL

M. Arias-Alvarez A, R. M. Garcia-Garcia A, L. Torres-Rovira B, A. Gonzalez-Bulnes B, P. G. Rebollar C and P. L. Lorenzo A

A Universidad Complutense de Madrid, Madrid, Spain;
B INIA, Madrid, Spain;
C Universidad Politecnica de Madrid, Madrid, Spain
   

Abstract
Export Citation
Print
  


Abstract

The effect of leptin on oocyte maturation and steroidogenic response could be mediated by activation of specific transcription factors. The aim of this work was to establish if the Signal Transducer and Activator of Transcription 3 (STAT3) pathway mediates leptin’s influence on oocyte maturation and steroid secretion of COC by using a rabbit oocyte model. A total of 522 COC from follicles >1 mm were IVM for 16 h (38°C, 5% CO2) in TCM-199 serum free medium plus: 0 or 10 ng mL-1 leptin. Each group was further treated with 10 and 100 μM of specific inhibitor for STAT3 pathway (AG490, Sigma, St. Louis, MO, USA). COC were treated progressively with hyaluronidase (2 mM), 0.5% pronase, 4% paraformaldehyde, 0.02% Triton X-100, and 7.5% BSA after IVM. Oocytes examined under a confocal laser-scanning microscope were previously incubated with 100 mg mL-1 fluorescein isothiocyanate of Lens culinaris (FITC-LCA) and then with 10 mg mL-1 propidium iodide, for cortical granule (CG) and nuclear staining, respectively. Spent maturation media were analyzed for oestradiol and progesterone secretion using Enzimoinmunoassay. Statistical analysis was performed by Chi-square and ANOVA tests. COC supplemented with 10 ng mL-1 leptin had significantly higher metaphase IIrate than those IVM without leptin (P < 0.05); but it did not enhance the percentage of oocytes with peripheral migration of CG (Table 1). Leptin-stimulated nuclear oocyte maturation was significantly impaired when the STAT3-inhibitor was added to the media (P < 0.005). CG migration index was not modified when inhibitors were included in IVM media compared to control groups. Estradiol and progesterone secretion by COC were similar in all groups tested; these concentrations were not affected when STAT3-pathway inhibitor was added. The results confirmed that 10 ng mL-1 leptin enhances meiotic oocyte maturation but not CG migration, through the STAT3 pathway. In the present rabbit model, this effect is not mediated by an increase of steroid secretion by COC.


We acknowledge CM, FSE, and MICINN for funding.

Reproduction, Fertility and Development 22(5305) 321–321   http://dx.doi.org/10.1071/RDv22n1Ab330
Published online: 08 December 2009




 
Top  Email this page
 
   


Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2014