CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Reproduction, Fertility and Development   
Reproduction, Fertility and Development
  Vertebrate Reproductive Science & Technology
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
Content
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Sample Issue
For Authors
General Information
Instructions to Authors
Submit Article
Open Access
For Referees
Referee Guidelines
Review an Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our email Early Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter youtube

red arrow Connect with SRB
blank image
facebook TwitterIcon

Affiliated Societies

RFD is the official journal of the International Embryo Transfer Society and the Society for Reproductive Biology.



Article << Previous     |     Next >>   Contents Vol 22(1)

415 INDUCTION OF FOLLICULAR WAVE EMERGENCE IN WOOD BISON BY FOLLICULAR ABLATION OR TREATMENT WITH ESTRADIOL AND PROGESTERONE

J. M. Palomino A, R. B. McCorkell B, B. Balog A, D. Ambati A, M. Woodbury A and G. P. Adams A

A University of Saskatchewan, Saskatoon, Canada;
B University of Calgary, Alberta, Canada
   

Abstract
Export Citation
Print
  


Abstract

Reclamation of Canada’s threatened wood bison (Bison bison athabascae) herd is complicated by endemic disease. As part of an overall goal to conserve bison genetics, the specific objective of this study was to develop a protocol to control follicular wave emergence in bison for the purposes of ovarian superstimulation and collection of disease-free oocytes. In an initial study, the synchronizing effect of follicular ablation and estradiol (E2) treatment was examined. Ablation was effective, but the effects of E2 (5 mg) were confounded by treatment-induced ovulation in some animals. Two experiments were done to determine the effect of a reduced dose of E2 or the addition of progesterone (P4) in comparison with follicular ablation. Bison cows (n = 19), ≥3 years old, were scanned for 14 days to determine the mean and variance in the interval to follicular wave emergence (control phase). In Experiment 1, bison were assigned randomly to 2 groups: follicular ablation (n = 9) or 2 mg of E2i.m. (n = 10). In Experiment 2, the same bison were randomly assigned to 2 groups: follicular ablation (n = 9) or 2 mg of E2+ 100 mg of P4 i.m. (n = 10). Ablation involved transvaginal ultrasound-guided aspiration of all follicles ≥5 mm. Wave emergence was determined retrospectively by identification of the follicle destined to become dominant at an initial diameter of 4 to 5 mm, with a concurrent increase in the number of follicles ≥4 mm. The interval and variation in the interval to emergence of a new follicular wave was compared among the control phase and treatment groups by ANOVA. In Experiment 1, the interval to new wave emergence (mean ± SEM) was 4.9 ± 0.66, 1.1 ± 0.11, and 2.4 ± 0.47 days from the start of the control phase, follicular ablation, and E2 treatment, respectively (P < 0.001). The degree of synchrony (residuals) was 2.4 ± 0.36, 0.2 ± 0.09, and 1.2 ± 0.24 days for the control phase, follicular ablation, and E2 treatment, respectively (P < 0.001). Ovulation was detected in 1 bison treated with E2. In Experiment 2, the interval to new wave emergence (mean ± SEM) was 4.9 ± 0.66, 1.2 ± 0.15, and 2.9 ± 0.31 days from the start of the control phase, follicular ablation, and E2 + P4 treatment, respectively (P < 0.001). The degree of synchrony was 2.4 ± 0.36, 0.2 ± 0.08, and 0.7 ± 0.20 days for the control phase, follicular ablation, and E2 + P4 treatment, respectively (P < 0.001), and no ovulations were detected. In conclusion, follicular ablation, E2, and E2 + P4 treatments all shortened and decreased the variability in the interval to new wave emergence in bison, but follicular ablation consistently produced a quicker and more synchronous response.


Supported by grants from the Advancing Canadian Agriculture and Agri-Food Fund (ACAAF), the Agri-Food Innovation Fund, Parks Canada, the World Wildlife Fund, and the Northwest Territories.

Reproduction, Fertility and Development 22(5305) 364–365   http://dx.doi.org/10.1071/RDv22n1Ab415
Published online: 08 December 2009




 
Top  Email this page
 
   


Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2014