
%0 Journal Article
%A Leng, RA 
%A Annison, EF 
%T Metabolic activities of sheep erythrocytes. I. Glycolytic activities
%J Australian Journal of Agricultural Research
%D 1962/02/01
%V 13
%N 1
%P 31-44
%K 
%R https://doi.org/10.1071/AR9620031
%U https://www.publish.csiro.au/paper/AR9620031 
%X Sheep erythrocytes, which in most animals are impermeable to glucose, show low glycolytic activities relative to human cells. When 14C-labelled glucose was incubated with erythrocyte suspensions the oxygen uptake was 10.9 &#177; 1.8 &#181;l/hr/ml of cells (5 replications), and glucose oxidation (measured by recovery of [14C]carbon dioxide) was 0.03 &#177; 0.007 &#181;mole/hr/ml (5). Addition of methylene blue (0.4 &#181;mole/ ml) increased oxygen uptake to 56 &#177; 3.5 &#181;l/hr/ml (5) and glucose oxidation to 0.36 &#177; 0.02 &#181;mole/hr/ml. Lactic acid production was increased from 1 .5 &#177; 0.06 &#181;mole/hr/ml (7) to 1.7 &#177; 0.11 &#181;mole/hr/ml (7) in the presence of methylene blue. Comparison of the yields of [14C]carbon dioxide from [1-14C]glucose and uniformly labelled [14C]glucose indicated that when stimulated by methylene blue 80&ndash;100&#37; of glycolysis proceeded by the pentose phosphate pathway, but in the unstimulated system the alternative aerobic pathway accounted for only about 15&#37; of total glycolysis. 
