
TY  - JOUR
A1  - Reid, RL 
T1  - Studies on the carbohydrate metabolism of sheep. XVI. Partition of ketone bodies in blood, tissues, and urine
JF  - Australian Journal of Agricultural Research
JO  - Aust. J.  Agric. Res.
Y1  - 1962
VL  - 13
IS  - 2
SP  - 307
EP  - 319
KW  - 
UR  - https://doi.org/10.1071/AR9620307 
N2  - Acetone comprised 0&ndash;40&#37; (average 18&#37;) of the acetoacetic acid plus acetone fraction in sheep blood, in which the level of this fraction was 0.6&ndash;5.2 mg &#37; (as acetone). Acetoacetic acid was largely converted to acetone during storage of blood at &ndash;20&#176;C, with intermittent thawing for analysis. Concentrations of acetoacetic acid in red cells were similar to those in plasma, but those of &#223;-hydroxybutyric acid were considerably lower. In contrast to acetoacetic acid, &#223;-hydroxybutyric acid was virtually absent from foetal blood and from brain tissue. Concentrations of both ketone fractions in liver and muscle tissue were about one-half the blood concentrations. The renal clearance of acetoacetic acid plus acetone in hyperketonaemic pregnant ewes was independent of blood level up to 20 mg &#37; and was little affected by rate of urine flow. Clearance values were in the range of 4&ndash;9 ml per min, which indicates that most of the acetoacetic acid filtered at the glomeruli is absorbed by the renal tubules. Renal clearance of &#223;-hydroxybutyric acid was dependent on blood level and was more affected by rate of urine flow than that of acetoacetic acid. Very little &#223;-hydroxybutyric acid appeared in the urine when blood levels were below 15 mg &#37;. Clearance increased as blood concentration rose above this level, and reached maximum values, mostly of 3&ndash;5 ml per min, at blood levels exceeding 30 mg &#37;. 
ER  -

